Microscope, micrometer, and bacterial motility Quiz

Question 1

the erratic motion of non-motile bacteria in an aqueous medium is due to ______

Answer 1

Brownian movement

Question 2

what is the total magnification for each lens setting on a microscope with 15X oculars and 4X, 10X, 45X, and 97X objectives lenses?

Answer 2

60X, 150X, 675X, and 1455X respectively

Question 3

which of the following accurately describes the concept of resolution

Answer 3

it refers to a lens’ ability to distinguish between adjacent objects

Question 4

UV light is a key component of _______

Answer 4

fluorescence microscopy

Question 5

the greatest resolution in light microscopy can be obtained with ______

Answer 5

shortest wavelength of visible light used and an objective with the max numerical aperature

Question 6

what unit of measurement is typically used on a stage micrometer

Answer 6

millimeters of micrometers

Question 8

why must calibration of the ocular micrometer be repeated for different objective lenses?

Answer 8

because the scale of the ocular micrometer changes with magnification

Question 9

which advantage does the hanging drop technique offer over a wet mount?

Answer 9

it enables better visualization of true bacterial motility

Question 10

what is the amin disadvantage of the wet mount technique?

Answer 10

the specimen may dry out quickly during observation