Microscope use

Question 1

important factors affecting image quality of microscope

Answer 1

enhancing resolution

contrast

Question 2

resolution def

Answer 2

ability to see details clearly

Question 3

contrast def

Answer 3

distinction between light and dark

Question 4

microscopes on campus are

Answer 4

monocular compound light microsopes

Question 5

monocular def

Answer 5

single eyepiece

Question 6

compound microscope def

Answer 6

image on a slide will pass through more than one lens

Question 7

ocular lens def

Answer 7

in eyepiece

Question 8

objective lenses

Answer 8

selected from one of the objectives

Question 9

mechanical stages feature

Answer 9

allow slides to be moved easily with knobs

Question 10

parafocal feature

Answer 10

the images stay nearly in focus when objective lensees are switched

Question 11

paracentric feature

Answer 11

once a sample is centered in the field of view, it will remain centered when switching to a higher magnification

Question 12

brightness control knob def & function

Answer 12

on/off switch at base of microscope
turning it will increase illumination from light bulb
recommend- turn it to 3/4 to end point to avoid creating a field that is too bright to regulate

Question 13

stage def

Answer 13

slide will be placed on this platform for viewing

Question 14

slide holder and claw

Answer 14

claw needs to be opened to position slide

Question 15

“X” & “Y” travel knobs

Answer 15

moves slide horizontally and vertically

Question 16

ocular lens and magnification

Answer 16

magnifying lens in eyepiece

magnifies image by 10X

Question 17

revolving nosepiece

Answer 17

disc is rotated when selecting and objective lens

Question 18

objective lenses

Answer 18

magnifying lenses used to further magnify the image

Question 19

magnification of each objective lense is

Answer 19

multiplied by 10, power of ocular lenses, to determine total magnification

Question 20

red lense magnification

Answer 20

Lens Power Ocular Power Magnification

4X 10X 40X

Question 21

yellow lense magnification

Answer 21

Lens Power Ocular Power Magnification

10X 10X 100X

Question 22

red lense name

Answer 22

scanning

Question 23

yellow lens name

Answer 23

low-power

Question 24

blue lense magnification

Answer 24

Lens power ocular power magnification

40X 10X 400X

Question 25

blue lens name

Answer 25

high-power

Question 26

white lens magnification

Answer 26

lens power ocular power magnification | 100X 10X 1000X

Question 27

white lens name

Answer 27

oil immersion

Question 28

order of magnification of lenses

Answer 28

red- scanning yellow- low- power blue- high-power white- oil immersion

Question 29

coarse and fine focus knobs function

Answer 29

used to adjust height of stage to bring the slide closer to the objective lens

Question 30

coarse focus knob used with which lens

Answer 30

lower power lenses

Question 31

coarse knob does

Answer 31

moves stage in larger increments than fine adjustment knob

Question 32

coarse knob should not be used when

Answer 32

the two higher power lenses are in place

Question 33

coarse and fine focus knob should never

Answer 33

be used at the same time

Question 34

no two adjustment knobs should

Answer 34

be used simultaneously

Question 35

iris diaphram def and function

Answer 35

lever located approx 1.5 in under stage | used to regulate the amount of light reaching the slide

Question 36

substage condenser def and function

Answer 36

does not magnify | condenses image so it can be seen

Question 37

substage condenser should not

Answer 37

be adjusted for routine use of microscope

Question 38

as power of lens inscreases, resolution...

Answer 38

decreases

Question 39

why does resolution decrease when power of lens increases

Answer 39

smaller surface area of stronger lenses | refraction of light waves as they pass between glass surfaces

Question 40

use of oil immersion in between slides does what

Answer 40

less light refracted | image quality improves

Question 41

immersion oil has almost the same refractive index as

Answer 41

glass, light behaves similarly when passing through both mediums

Question 42

use of oil allows

Answer 42

to magnify specimen 1000X with some clarity

Question 43

using 1000X lens without oil

Answer 43

very unclear image due to excessive light refraction

Question 44

steps of use of highest power objective lens, oil immersion

Answer 44

1. use nosepiece to postition scanning lens on one side of slide and oil immersion lens on other side 2. apply drop of immersion oil to slide, using incoming ligth as target 3. rotate nosepiece to click immersion lens in place over slide 4. use fine focus knob and iris diaphragm to improve image 5. can go back and forth between scannning power and oil lens to refine image 6. immersion oil lens makes contact with drop of oil

Question 45

if you lose focus with oil immersion go back to

Answer 45

scanning power, back and forth