Microscopes Flashcards
How do you carry the microscope?
carry the microscope with both hands; one on the arm and the other under the base. Do not tip the microscope because the eye pieces may fall out. Set it down gently do not bump or drag the microscope
What is used to clean lenses?
- lens paper
* solvents
List what must be done when putting away a microscope:
- Clean every lens with lens paper
- leave the 4x objective lens facing down
- Move the mechanical stage to center, so nothing sticks out at the side
- Open the iris diaphragm all the way (lever all the way to the right)
- Rotate coarse focus adjustment knob so that the stage is ~1 inch from the lamp source
- Set light intensity to the lowest setting and turn the light source switch off
- Set filter to O (bright field)
- Coil and bind cord
- Put microscope in correct slot.
Compound Light microscope
a microscope which uses a light source to illuminate the specimen from below and a series of lenses to enlarge the image of the specimen.
Compound*
2 systems of lenses
Binocular*
2 eye pieces
Parafocal*
A parfocal lens is a lens that stays in focus when magnification/focal length is changed. There is inevitably some amount of focus error, but small enough to be considered insignificant. When stage is set in scanning it is at the appropriate hight for all objectives only fine adjustments should be made.
e- microscope
passes e- beams through specimen, specimen must be covered in metal, allows us to achieve greater magnification than light microscopes
Fixed specimen
a specimen prepared in advanced and used repeatedly
wet-mount/ smear
a specimen that is collected and prepared immediately by the user.
Light passes through the slide into the ___________
objective lens
The longer the objective lens the ___________
the higher the magnification power; smaller the working distance.
Working distance
the space between the slide and the objective lens
The user observes specimens by looking through the ______.
ocular lens
What is the magnification power of the ocular lens?
10x
pointer
a feature of an ocular lens which allows a user to point to a specific area on a slide
One of the ocular lenses will ____ to improve _________.
rotate
resolution
Ocular micrometer
a ruler found in the ocular lens which allows the user to take measurements of a specimen, must be calibrated for each objective lens.
Total magnification
The enlarged size of an object verses its total size when viewed through the objective and ocular lens.
Resolution
How clear the image of the specimen appears
Contrast
How well you are able to determine different parts of the microscope.
Field of view
The area that can be seen with the microscope
image orientation
mirrors are used to observe the specimen so the image will appear backwards and upside-down.
Ocular lens
also called the eye piece, found at the top of the microscope and magnifies the specimen 10x.
