microscopes

Question 1

magnification

Answer 1

the ratios of the image size to the object size

image size/ actual size

Question 2

resolution

Answer 2

the ability to distinguish two separate points that are close together

Question 3

light microscopes

Answer 3

1000-2000x magnification
50-200nm resolution
used to view tissue cultures and cells

Question 4

scanning electron microscope

Answer 4

50,000-500,000x magnification
0.4-20nm resolution
viewing the surface of cells and organelles
providing depth/three dimensional images

Question 5

transmission electron

Answer 5

300,00-1,000,000 magnification
0.05-1nn
detailing organelles

Question 6

laser scanning confocal

Answer 6

1000-2000x magnification
50-200nm resolution
three dimensional images with good depth selection

Question 7

light microscope evaluation

Answer 7

😊 cheap and easy to use
😊allows us to see living L things

😫resolution is limited

Question 8

evaluation of scanning and transmission electron microscopes

Answer 8

😊best resolution
😊gives 3D images with depth of field
😊scanning is good for viewing surface

😫very large and expensive
😫requires a trained operative
😫the sample must be dried therefore it is dead and can change the shape of the image

Question 9

evaluation of laser scanning confocal

Answer 9

😊can focus at specific depth so the image is not confused by other components
😫 the image is an interpretation rather than a real life image

Question 10

staining

Answer 10

the application of stains to the tissue or cells

makes objects visible in light microscopes
increases contrast so object is seen more clearly
is often specific to certain tissues or organelles