Microscopes And Calibration

Question 1

Define magnification

Answer 1

Number of times larger an object is compared with the real size of the object

Question 2

Define resolution

Answer 2

The ability to distinguish between 2 points

Question 3

What are the properties of electron microscopes?

Answer 3

• Greater magnification
• Greater resolution
• More expensive
• More complex prep
• Uses electrons
• Focus through electro magnet

Question 4

What are the properties of light/optical microscopes?

Answer 4

• Lower magnification
• Lower resolution
• Cheaper
• Easier to use
• More simple prep
• Focus through glass objective lens

Question 5

What are the 2 types of electron microscopes?

Answer 5

Transmission electron microscope (TEM) & scanning electron microscope (SEM)

Question 6

What are the features of TEM?

Answer 6

• Beam of electrons pass through specimen
• greater resolution than SEM
• able to observe ultra structure (greater detail)
• Black and white

Question 7

What are the features of SEM?

Answer 7

• Beam of electrons scan the surface of specimen
• resolution poorer than TEM
• surface/structure of organelle able to be seen, great depth of field
• colour image
• 3D image