Microscopy Flashcards
(40 cards)
Microscope Magnification
the number of time s the image of the specimen is made larger.
field of view
maximum area you can see through the ocular lens
specimen size
the actual size of the specimen being viewed
drawing magnification
the number of times larger a drawing is drawn compared to specimen size
resolution
point at which two objects can be distinguished separate
compound microscope
light rays are passed through
internal structures
cells and organisms can be observed while still living
dissecting microscope
light rays are bounced off a specimen
external structures
can be observed still living
transmission electron microscope (TEM)
beams of electrons are passed through thin sections of a specimen
internal structures of very small organisms may be viewed
images are projected onto computer or television screen
max magnification: ~1000000
Limit of resolution : 0.0000007
Scanning Electron Microscope (SEM)
Beams of electrons are bounced of specimens surface
external structures of very small organisms may be viewed
images are projected onto computer or television screen
max magnification: ~1000000
Limit of resolution : 0.0000007
Base
Bottom of microscope
Supports the microscope
arm
supports the body tube, objective lens and ocular lens as well
body tube
Connects the ocular lens to the objective lenses
coarse adjustment focusing knob
moves the stage closer and further from the lenses. general focus
fine adjustment focusing knob
an adjustment that is used for higher level lens to fine focus the image. Detail focus
low power objective lens
magnifies image 4X
medium power objective lens
magnifies image 10X
high power objective lens
magnifies image 40X
oil immersion objective lens
magnifies image 100X
ocular lens
lens at the top that you look through magnifies 10X
stage
the platform where the slides are placed
stage clips
holds the slides in place
revolving Nosepiece
holds objective lenses in place and revolves them
diaphragm
used to vary the intensity and the size of the cone of light that is projected upward into the slide
lamp
light source