Microscopy Flashcards
1
Q
Magnification
A
How many times larger the image is than the actual size of the object being viewed.
2
Q
Resolution
A
The ability to see individual objects as separate entities.
The Lowe the resolution, the better
3
Q
Magnification Formula
A
Magnification=Size of image/Actual size of object.
4
Q
Light microscope (normal)
A
- Low resolution of 0.2 micrometers (um).
- Maximum useful magnification of X1500.
- Used to observe whole cells.
5
Q
Laser scanning confocal microscopes.
A
- Causes dye to give off light.
- 3D image.
- Out of focus light is blocked, producing a clear image.
- Look at different depths in thick specimen.
6
Q
Transmission Electron Microscope (TEM)
A
- Maximum resolution of 0.0002um.
- Maximum magnification of X1,000,000
- Only 2D images.
- Used to observe organelles.
7
Q
Scanning electron microscope (SEM).
A
- Maximum resolution of 0.002 um
- Maximum magnification of X 500,000
- Produces 3D images.
8
Q
Reason for staining
A
- Staining increases contrast.
- Makes it easier to recognise different organelles.
9
Q
How to prepare a microscope slide
A
- Use tweezers to pick up the specimen and place it in the middle of a clean slide.
- Put a cover slip on top of the specimen.
10
Q
How to use a Light microscope.
A
- Clip the slide onto the stage.
- Select the objective lens with the lowest magnification.
- Use the Coarse adjustment knob to bring the stage up to just below the objective lens.
- Use the coarse adjustment knob to lower the stage, away from the objective lens until the image is in focus.
- Swap to an objective lens with a higher magnification to see the slide with a greater magnification.
11
Q
How to use an eyepiece graticule and a state micrometer.
A
- Line up the eyepiece graticule and the stage micrometer.
- Each division of the stage micrometer is 0.1mm long.
- At this magnification, 1 division of the stage micrometer=4.5 divisions on the eyepiece graticule.
- Division on the stage micrometer/divisions on the eyepiece graticule(0.1/4.5=0.022 mm)
