Microscopy

Question 1

3 parts of a Compound Microscope

Answer 1

1. Magnifying parts
2. Illuminating parts
3. Mechanical parts

Question 2

2 parts of Magnifying parts

Answer 2

1. Ocular lens
2. Objectives

Question 3

4 Objectives

Answer 3

1. Scanner
2. Low Power Objectives (10x)
3. High Power Objectives (40x)
4. Oil Immersion Objectives (100x)

Question 4

3 parts of Illuminating Parts

Answer 4

1. Light source
2. Condenser
3. Iris Diaphragm

Question 5

3 parts of Mechanical Parts

Answer 5

1.Base
2. Arm
3. Stage

Question 6

6 types of Microscope

Answer 6

1. Bright-field Microscope
2. Dark-field Microscope
3. Fluorescence Microscope
4. Phase Contrast Microscope
5. Uv Microscope
6. Electron Microscope

Question 7

It supports the entire microscope.

Answer 7

Base

Question 8

It supports the upper part of the microscope.

Answer 8

Arm

Question 9

It holds the specimen place and allows navigations.

Answer 9

Stage (knobs,clips)

Question 10

It allows the elevation of stage and assists or support focusing.

Answer 10

Course & Fine Focus Adjustment

Question 11

It controls the amount of light.

Answer 11

Condenser

Question 12

It controls the radius of the light beam.

Answer 12

Iris Diaphragm

Question 13

background is light; specimen is dark (stains required)

(types of microscope)

Answer 13

Bright-field Microscope

Question 14

background is dark; specimen is bright (urine, crystals, unstained)

(types of microscope)

Answer 14

Dark-field Microscope

Question 15

background is dark; specimen is light or glowing; stains required

(types of microscope)

Answer 15

Fluorescence Microscope

Question 16

uses polarize light

(types of microscope)

Answer 16

Phase-contrast Microscope

Question 17

always colorless (scanning & transmission)

(types of microscope)

Answer 17

Electron Microscope

Question 18

An instrument for viewing objects that are too small to be seen by the naked or unaided eye.

Answer 18

Microscope

Question 19

The science of investigating small objects
using such an instrument is called microscopy.

Answer 19

Microscopy

Question 20

first compound microscope (year)

Answer 20

1590

Question 21

Light was refracted when it travels from one medium to another.

Answer 21

Huygen’s Wave Theory

Question 22

He made the first recorded microscopic observation.

Answer 22

Robert Hooke

Question 23

He observed single cell organism (microbes).

Answer 23

Anton Van Leeuwenhoek

Question 24

He compound microscope.

Answer 24

Galileo Galilei - 1609

Question 25

Compound microscope made by whom.

Answer 25

John Cuff 1750

Question 26

Absorbs light at a shorter wavelength (UV Light) and emits light at a longer one (visible light). [types of microscope]

Answer 26

Fluorescence Microscope

Question 27

Optical system to enhance the contrast of unstained bodies. [types of microscope]

Answer 27

Dark-field microscope

Question 27

It enables visualization of internal cellular components. (organelles). [types of microscope]

Answer 27

Phase-contrast microscope

Question 28

Produces high-contrast images of transparent specimens. [types of microscope]

Answer 28

Phase-contrast microscope

Question 29

"micron" means

Answer 29

small

Question 30

"aim" means

Answer 30

scopose

Question 31

1590 — They developed first microscopee.

Answer 31

Hans Janssen & Zacharias Janssen

Question 32

1609 — He compound microscope

Answer 32

Galileo Galilei

Question 33

1620 — developed a simple 2-lens ocular system that was chromatically corrected.

Answer 33

Christian Huygens

Question 34

He discovered bacteria, free-living and parasitic microscopic protists, sperm cells, blood cells, microscopic nematodes etc.

Answer 34

1661 — Anton Van Leeuwenhoek

Question 35

5 techniques tissue preparation

Answer 35

1. Fixation 2. Dehydration and Clearing 3. Embedding 4. Sectioning and Mounting 5. Staining

Question 36

The tissues are mechanically and biochemically stabilized in a fixative. [techniques tissue preparation]

Answer 36

Fixation

Question 37

The samples are immersed in multiple baths of progressively more concentrated ethanol to dehydrate the tissue, followed by a clearing agent such as, xylene or Histoclear. [techniques tissue preparation]

Answer 37

Dehydration and Cleaning

Question 38

During this 12 to 16 hour process, paraffin wax will replace the water: soft, moist tissues are turned into a hard paraffin block, which is then placed in a mould containing more molten wax (embedded) and allowed to cool and harden. [techniques tissue preparation]

Answer 38

Embedding

Question 39

The tissue is then sectioned into very thin (2 -8 micrometer) sections using a microtome [techniques tissue preparation]

Answer 39

Sectioning & Mounting

Question 40

To see the tissue under a microscope, the sections are stained with one or more pigments. [techniques tissue preparation]

Answer 40

Staining

Question 41

2 types of Staing

Answer 41

1. Basophilic 2. Acidophilic

Question 42

nuclear components; hematoxylin. [types of stain]

Answer 42

Basophilic

Question 43

cytoplasmic components; eosin [types of stain]

Answer 43

Acidophilic