microscopy

Question 1

Provides morphology and arrangement

Answer 1

Simple stain

Question 2

Provides gram reaction, morphology and arrangement

Answer 2

Differential stain

Question 3

Simple staining method

Answer 3

Malachite green
safranin
methylene blue

Question 4

Prepare smear from clinical specimens

Answer 4

Direct smear

Question 5

Prepare smear from culture

Answer 5

Indirect smear

Question 6

Comma shaped bacilli is called

Answer 6

Vibrio

Question 7

What is not usually encountered and where gram stain is not commonly done

Answer 7

Spiral

Question 8

Micrococcus tetragena

Answer 8

Gram positive cocci in tetrads

Question 9

Example of gram positive cocci in pairs (bean-shaped)

Answer 9

Streptococcus pneumoniae

Question 10

Example of gram negative cocci in pairs (commonly found intracellulary inside PMN)

Answer 10

Neisseria spp.
- Neisseria meningitidis
- Neisseria gonorrhoeae

Question 11

Sarcina lutea

Answer 11

Gram positive cocci in sarcinae (cubical packet)

Question 12

Streptococcus

Answer 12

Gram positive cocci in chains

Question 13

Staphyloccocus

Answer 13

Gram positive cocci in clusters

Question 14

Example of gram positive bacilli in chains

Answer 14

Bacillus

Question 15

Non-fermenters such as Pseudomonas

Answer 15

Gram negative bacilli in pairs

Question 16

Enterics

Answer 16

Gram negative bacilli in pairs, singles

Question 17

Example of gram positive bacilli that are large and arranged singly or individually with spores

Answer 17

• Clostridium tetani, Clostridium botulinum
• Clostridium perfringens

Question 18

Box-car appearance bacilli

Answer 18

Clostridium perfringens

Question 19

Whats the smallest pathogenic bacilli in micrometre

Answer 19

Haemophilus ducreyi

(02.x05 um)

Question 20

Haemophilus ducreyi

Answer 20

Gram negative coccobacilli straight and rod shaped

Question 21

Bacteroides fragilis

Answer 21

Gram negative bacilli with rounded ends

Question 22

Gram positive irregular bacilli “club shaped” arranged in Chinese characters x, or y and in palisade, snapping arrangement

Answer 22

Corynebacterium

Question 23

Gram positive branching bacilli with bifurcated or spatulated ends

Answer 23

Bifidobacterium

Question 24

Gram positive bacilli which is slender, branching and filamentous; partially acid fast

Answer 24

Nocardia

Question 25

Gram positive filamentous bacilli with a V-shaped pattern

Answer 25

Erysipelothrix rhusiopathiae

Question 26

Streptobacillus moniliformis also known as

Answer 26

Necklace bacillus

Question 27

Example of gram negative bacilli arranged in long looped filamentous forms

Answer 27

Streptobacillus moniliformis

Question 28

Gram negative fusiform bacilli

Answer 28

Fusobacterium nucleatum

Question 29

Gram negative comma shaped bacilli in singles

Answer 29

Vibrio

Question 30

Gram negative seagull wing shaped or s-shaped bacilli

Answer 30

Campylobacter

Question 31

Gram negative curved bacilli with two or more curves

Answer 31

Spirillum

Question 32

Acid fast bacilli - slender, long, arranged in serpentine cord like pattern

Answer 32

Mycobacterium tuberculosis

Question 33

Acid fast bacilli - slender and in palisades / globi packets / cigar bundle; arranged singly

Answer 33

Mycobacterium leprae

Question 34

Gram negative spiral shaped bacteria - stained with silver, fluorescent or tissue stains such as giemsa

Answer 34

Spirochets Leptospira interrogans Treponema pallidum Borrelia

Question 35

Gram positive (not bacteria)

Answer 35

Yeast cells and pseudohypha

Question 36

Square shaped bacteria

Answer 36

Haloarcula spp.

Question 37

Star shaped

Answer 37

Stella spp.

Question 38

In DSSM, ______ should be read before reporting the sputum smear as negative for AFB

Answer 38

300 OIF

Question 39

Spiral shaped structures that can be seen in asthmatics

Answer 39

Curschmann’s spirals