Microscopy 2 Flashcards
(17 cards)
What is the purpose of a microscope slide?
To mount a specimen for viewing under a microscope
What is a drop of iodine solution used for in slide preparation?
As a stain to highlight objects in a cell by adding colour
What is the first step in preparing a slide to view onion cells?
Add a drop of water to the middle of a clean slide
How should you place the cover slip on the slide?
Stand it upright next to the water droplet, then tilt and lower it
What should you avoid when placing the cover slip on the slide?
Getting air bubbles under the cover slip
What is the first step to use a light microscope after preparing your slide?
Clip the slide onto the stage
What type of objective lens should you select first when using a light microscope?
The lowest-powered objective lens
What is the function of the coarse adjustment knob?
To move the stage up or down to focus the image
How should you adjust the focus once the image is roughly in focus?
Use the fine adjustment knob
What should your drawing of the observed specimen include?
A title and the magnification under which it was observed
Fill in the blank: Stains are used to highlight objects in a cell by adding _______.
[colour]
What should you use to draw your observations under the microscope?
A pencil with a sharp point
What is the formula to calculate magnification?
Magnification = length of drawing of cell ÷ real length of cell
True or False: Your drawing should include colouring or shading.
False
What should the proportion of subcellular structures in your drawing reflect?
The actual proportions of the structures
What is the real length of an onion cell if the drawing is 33 mm and the magnification is x 110?
0.3 mm
What is the importance of being able to use a microscope?
It is a key skill for scientific exploration and observation
