Microscopy and Cell membranes Flashcards

(35 cards)

1
Q

What are the elements of ‘central dogma’ presented in the correct temporal order?

A

DNA, RNA, protein.

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2
Q

What are the oldest known fossils?

A

stromatolites, rocks formed from accumulation of sedimentary layers on bacterial mats. 3.5B years.

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3
Q

What is central dogma?

A

The sequence of nucleotides in a particular segment of DNA (a gene) is transcribed into an RNA molecule, which can then be translated into the linear sequence of amino acids of a protein.

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4
Q

Living _____ are self-___________ collections of ________.

A

cells, replicating catalysts.

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5
Q

What are the domains of prokaryotes?

A

Bacteria
Arachae

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6
Q

What is the basic prokaryotic cell architecture?

A

NAME?

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7
Q

What is the function of the nucleus?

A

Information store of the cell.

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8
Q

How to mitochondria generate usable energy?

A

From food molecules.

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9
Q

Where the the energy captured from in chloroplasts?

A

Sunlight.

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10
Q

What do internal membranes create in a eukaryotic cell?

A

Intracellular compartments

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11
Q

What is the cytoskeleton responsible for?

A

Directed cell movements.

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12
Q

What does it mean if a nuclear membrane is non - continuous?

A

It has pores in the membrane.

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13
Q

Name the parts of the eukaryotic cell

A

Lysosome, mitochondrion, nuclear envelope, peroxisome, golgi apparatus, transport vesicle, endoplasmic reticulum, cytosol, plasma membrane.

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14
Q

Where does the double membrane from mitochondria come from?

A

From plasma membrane and outer membrane of the engulfed bacterium.

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15
Q

What did chloroplasts evolve from?

A

Photosynthetic bacteria, they already contained mitochondria.

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16
Q

What is the golgi apparatus composed of?

A

A stack of flattened, membrane-enclosed discs.

17
Q

What are the 3 major types of filaments of the cytoskeleton?

A

Actin filaments
Microtubules
Intermediate filaments.

18
Q

How can the filaments of the cytoskeleton be detected?

A

Fluorescent stains.

19
Q

What can be described as the smallest living unit?

20
Q

Which cytoskeletal element is the thickest?

21
Q

Which biochemical process correlates with the tenets of the central dogma?

A

DNA->RNA->Proteins
equals
replication->transcription-> translation

22
Q

What unit of length would you ordinarily use to measure to measure a typical plant or animal cell?

23
Q

Why is the resolving power of a fluorescent microscope so similar to a light microscope?

A

The fluorescent microscope’s resolving power limited by the wavelength of visible light.

24
Q

How many membranes is the nuclear envelope made up of?

A

Two concentric membranes.

25
The nuclear envelopes concentric membranes are continous with which other membrane?
Endoplasmic reticulum
26
The nucleus confines what cellular component? keeping them separated from the other components of the cell
Chromosomes.
27
What organelle has both an inner and outer membrane?
Mitochondrion
28
What are the components of a conventional light microscope?
Eyepiece, Tube lens, Objective lens, Glass slide, Specimen, Condenser lens, light source
29
What is the magnification of a conventional light microscope?
x1000
30
What is the resolving power of a conventional light microscope?
0.2µm(200nm)
31
What allows for easy access of nutrients for growth of cancer cells?
Blood vessels with large lumen and a very thin vascular wall, also allows a pathway for cancer cells to enter blood circulation(metastasis).
32
What are the components of a fluorescent microscope?
Eyepiece, light source, beam-splitting mirror, objective lens, object, filter,
33
What do the two different filters in a fluorescent microscope for?
1. Filter light before it reaches specimen so that only wavelengths that excite the die are passed through. 2. Blocks out light and passes only wavelengths emitted when the dye fluoresces. Dyed object show up in bright colour on a dark background.
34
What are the parts of the scanning electron microscope?
Electron gun, condenser lens, beam deflector, objective lens, scan generator, video screen, detector, electron beam, specimen.
35
What are the parts of the transmission electron microscope?
Electron gun, condenser lens, specimen, objective lens, projector lens, viewing screen or photographic film.