Microtomy

Question 1

Rocking microtome was invented by

Answer 1

Paldwell trefall

Question 2

Thickness of cambridge

Answer 2

10-12 um

Question 3

Tissues are cut in slightly curved planes thus not recommended for serial sections

Answer 3

Rocking micro

Question 4

Most common and its thickness

Answer 4

Rotary; 3-5 um

Question 5

Used for routine and research laboratories

Answer 5

Rotary

Question 6

Rotary invented by

Answer 6

Minot 1885-86

Question 7

Knife and block holder are brought together by upward and vertical motions, cutting sections in a perfectly flat plane;

Answer 7

Rotary

Question 8

Sliding microtome developed by

Answer 8

Addams 1789

Question 9

√ electrically
driven and ideal for RESIN-EMBEDDED
decalcified bone.

Answer 9

Base sledge microtome

Question 10

knife is moving; the MOST DANGEROUS type of microtome because of the exposed, movable blade.

Answer 10

STANDARD SLIDING MICROTOME

Question 11

Recommended for cutting “extremely” hard and rough tissue blocks

Answer 11

SLIDING

Question 12

Recommended for cutting “extremely” hard and rough tissue blocks

Answer 12

SLIDING

Question 13

Both Microtome, the knife can be set OBLIQULEY for celloidin or straight large paraffin.

Answer 13

SIIDING

Question 14

Freezing microtome invested by

Answer 14

Quickett 1848

Question 15

Freezing agent of freezing microtome

Answer 15

Co2

Question 16

Used to cut undehydrated tissues in a frozen state.

Answer 16

Freezing micro

Question 17

Ideal for fresh tissues that did NOT UNDERGO FIXATION down to INFILTRATION.

Answer 17

FREEZING MICRO

Question 18

Used for RAPID preparation of URGENT tissue biopsies
for intraoperative diagnosis (STAT).

Answer 18

CRYOSTAT

Question 19

Chamber of cryosat temp

Answer 19

-5 to -30C

Question 20

Capable of freezing fresh tissue within 2-3 minutes.

Answer 20

Thermostat

Question 21

Cutting section of thermostat

Answer 21

4um

Question 22

Fluorescent antibody staining technique or histochemical enzyme studies.

Answer 22

Cryostat

Question 23

For electron microscopy, tissues fixed with osmic
acid.

Answer 23

Ultrathin micro

Question 24

Media of uultrathin micro and thickness

Answer 24

Plastc; 0.5 um

Question 25

Uses diamond knife

Answer 25

Ultrathin

Question 26

Types of tissue sections

Answer 26

Paraffn Celloidin Frozen sections

Question 27

Sections are removed in RIBBONS OF TEN to allow easy location of serial sections

Answer 27

Paraffin

Question 28

Thickness of paraffin

Answer 28

4-6um

Question 29

Thickness of celloidin

Answer 29

10-15 um

Question 30

To avoid dehydration and shrinkage, sections are usually cut by the WET method,

Answer 30

CELLOIDIN

Question 31

In celloidin, sections and the block being kept moist with — during cutting. Sections do not come off in -

Answer 31

70% alcohol Ribbons

Question 32

Microtome knives

Answer 32

Plane-concave Biconcave Plane-wedge

Question 33

Widely use blade for 10 tissue blocks

Answer 33

Disposable blades

Question 34

Disposable blade is coated w

Answer 34

Polytetrafluoroethylene

Question 35

For ultrathin microtomes.

Answer 35

Glass knive/ralph knive

Question 36

For RESIN blocks on ultrathin microtomes; ● Brittle and expensive

Answer 36

Diamond knives

Question 37

Blades For partially calcified materials, paraffin, and frozen sections.

Answer 37

Safety razor blades

Question 38

Blades Unsatisfactory for sections less than 10 micro.

Answer 38

Safety razor blade

Question 39

Bevel angle

Answer 39

27-32

Question 40

Cutting angle

Answer 40

14

Question 41

Angle between the UPPER facet of the knife and the SURFACE of the tissue block.

Answer 41

Cutting angle

Question 42

Angle between the LOWER facet of the knife and the SURFACE of the tissue block;

Answer 42

CLEARING ANGLE

Question 43

Clearing angle should be inclined at

Answer 43

5-10

Question 44

2 stages of sharpening

Answer 44

Honing Stropping

Question 45

Removing of GROSS nicks

Answer 45

Honing

Question 46

HEEL TO TOE

Answer 46

HONNING

Question 47

TOE TO HEEL

Answer 47

STROPPING

Question 48

Final polishing of the knife edge

Answer 48

STROPPPING

Question 49

Removal of burr/irregularities

Answer 49

STROPPING

Question 50

Knife sharpeners for finall polishing

Answer 50

Diamantine

Question 51

— is used in stropping

Answer 51

Paddle strop

Question 52

Never used with Pap Staining Method as it will retain OG stain;

Answer 52

Mayer’s egg albumin

Question 53

Outdated blood stored in blood banks, dispensed into sterile tubes of 0.5 ml each.

Answer 53

Plasma

Question 54

Better adhesion than albumin

Answer 54

1% Gelatin

Question 55

Does NT RETAIN the stain.

Answer 55

1% METHYL CELLULOSE

Question 56

ADHESIVE No background staining

Answer 56

Poly-L Lysine

Question 57

Slight dye retention, not affected by mild alkaline solutions 3 months. STRONG ADHESIVE

Answer 57

SODIUM SLICATAE

Question 58

Diluted 1:10 with acetone;

Answer 58

RESIN

Question 59

Selected sections for staining should fish-out in a — POSITION

Answer 59

VERTICAL

Question 60

Sections are floated out on a water bath set at

Answer 60

45-50°C.

Question 61

Temp of slide dryer

Answer 61

5-10°C above the melting point of paraffin

Question 62

In drying slides, hot plates are not recommended because

Answer 62

Overheating Dust falling onto section

Question 63

Mounted sections are placed in — for - miutes

Answer 63

Paraffin oven; 5 min

Question 64

Process of trim and embedding

Answer 64

Trim tissue Cool block w ice Cut tissue -> make ribbons Float tse in water bath Pick up tissue on slide Dry

Question 65

Ideal shape in trimming

Answer 65

Truncated pyramid