MID YEAR - Plasmids

Question 1

What is a plasmid?

Answer 1

A plasmid is a small, circular piece of DNA found in bacteria that is separate from the chromosomal DNA.

Question 2

True or False: Plasmids can replicate independently of chromosomal DNA.

Answer 2

True

Question 3

Fill in the blank: Plasmids are commonly used in __________ for genetic engineering.

Answer 3

molecular cloning

Question 4

What type of organisms primarily contain plasmids?

Answer 4

Bacteria

Question 5

Multiple Choice: Which of the following is NOT a function of plasmids? A) Antibiotic resistance B) Protein synthesis C) Photosynthesis D) Gene transfer

Answer 5

C) Photosynthesis

Question 6

What is the role of plasmids in antibiotic resistance?

Answer 6

Plasmids can carry genes that provide bacteria with resistance to antibiotics.

Question 7

True or False: Plasmids can be transferred between bacteria through a process called conjugation.

Answer 7

True

Question 8

What are the two main types of plasmids?

Answer 8

Conjugative plasmids and non-conjugative plasmids.

Question 9

Fill in the blank: __________ plasmids are capable of transferring themselves to other bacteria.

Answer 9

Conjugative

Question 10

Short Answer: How do scientists use plasmids in genetic engineering?

Answer 10

Scientists use plasmids to insert and replicate genes of interest in host cells.

Question 11

Multiple Choice: Which of the following is a common method for introducing plasmids into bacterial cells? A) Filtration B) Transformation C) Evaporation D) Precipitation

Answer 11

B) Transformation

Question 12

What is a recombinant plasmid?

Answer 12

A recombinant plasmid is a plasmid that has been artificially modified to contain DNA from another source.

Question 13

True or False: Plasmids can exist in eukaryotic cells.

Answer 13

True, but they are primarily found in bacteria.

Question 14

Fill in the blank: Plasmids can be used to produce __________ proteins in biotechnology.

Answer 14

recombinant

Question 15

Short Answer: What is the significance of plasmids in biotechnology?

Answer 15

Plasmids are essential tools for gene cloning, protein production, and genetic modification.

Question 16

Multiple Choice: Which of the following plasmids is commonly used as a cloning vector? A) pBR322 B) pUC19 C) Both A and B D) None of the above

Answer 16

C) Both A and B

Question 17

What is the main advantage of using plasmids in research?

Answer 17

Plasmids allow for the easy manipulation and replication of genetic material.

Question 18

True or False: Plasmids can carry multiple genes.

Answer 18

True

Question 19

Fill in the blank: Plasmids can be engineered to contain __________ sequences for specific functions.

Answer 19

promoter

Question 20

Short Answer: Name one way plasmids can contribute to the development of vaccines.

Answer 20

Plasmids can be used to produce antigens that trigger an immune response.