Midterm 1

Question 1

Hendersen-Hasselbalch

Answer 1

pH=pKa+ log ([base]/acid])

Question 2

Aspartic Acid

Answer 2

3.9

Question 3

Glutamic Acid

Answer 3

4.2

Question 4

Histidine

Answer 4

6.0

Question 5

Arginine

Answer 5

12.5

Question 6

Cysteine

Answer 6

8.3

Question 7

Tyrosine

Answer 7

10.1

Question 8

Lysine

Answer 8

10.0

Question 9

Ion Exchange Chromotography

Answer 9

Filter based on charge of protein.
Anion, negative elutes last.
Cation, positive elutes last.

Question 10

Trypsin

Answer 10

N:Arg/Lys
C:NOT Pro

Question 11

Chymotrypsin

Answer 11

N:Phe/Trp/Tyr/Leu
C:NOT Pro

Question 12

Thrombin

Answer 12

N:Arg
C:NOT Pro

Question 13

Themolysin

Answer 13

N:NOT Pro
C: Leu/Val/Ile/Met

Question 14

V-8

Answer 14

Asp/Glu

Question 15

Supersecondary Structures

Answer 15

aaa, bab,aa hairpin, greek key

Question 16

Domains

Answer 16

aa,bb, a/b. One folded section of one protein.

Question 17

Serine Proteases Transition State

Answer 17

Oxyanion hole transition state

Question 18

Below Pka

Answer 18

More protanated

Question 19

Above Pka

Answer 19

Less protanated

Question 20

pH Below PI

Answer 20

Positively charged

Question 21

pH Above PI

Answer 21

Negatively charged

Question 22

Amino, Imadazole (H), Guanidnium (R)

Answer 22

Below pKa charge of +1, otherwise 0

Question 23

Hydroxyl, SH, Carboxylate

Answer 23

Above pKa charge -1

Question 24

O,S in AA pH

Answer 24

Above subtract

Question 25

H in AA pH

Answer 25

Below, add

Question 26

base/acid=fraction

Answer 26

subtract from pKa

Question 27

base/acid=whole nuumber

Answer 27

add to pKa

Question 28

a-helices

Answer 28

Right-handed 100 degree phi=-57degree psi= -47 degree O in first residue H bonds with NH is fourth residue

Question 29

B-Sheets

Answer 29

Left handed twist | Beta strand right hand twist

Question 30

Actin

Answer 30

Helical, right handed twist between two fibers | 715 A pitch

Question 31

Ki (competitive inhibition)

Answer 31

concentration of enzyme mult by inhibitor divided by how much of the complex is there

Question 32

a(enzymes comp inhibition)

Answer 32

1+ [I]/ki

Question 33

a'(enzymes) (uncomp inhib)

Answer 33

concentration of enzymesub times inhib/ concentration of complex

Question 34

Ki'(uncomp inhib)

Answer 34

1+ [I]/Ki'