Midterm Flashcards

(114 cards)

1
Q

What are the 5 senses

A

Gustation - Taste
* Olfaction - Smell
* Audition - hearing
* Vision - eyes
* Tactile - touch

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2
Q

What are the two thesis with tactile

A

tactile - touch
somesthesis – feelings along the body surface
– kinesthesis – feelings of muscles

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3
Q

For a sensation to occur what must happen?

A

There must be a stimulus
2. Need to have a receptor
3. Conducted along a neural pathway
4. Brain translates the message/impulse

For example if you eat a chilli the stimulus needs to react with the receptors on your tongue and then a neural pathway is needed to conduct the signal along the neural pathway to the brain which translates the message/impulse like oh hey that’s spicy

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4
Q

Why do we taste?

A

To Prepare the body to digest food
* Trigger cephalic phase responses.
Indicate food’s nutritional value.
Used in the development of food preferences. (psychological)
Enhance feelings of satiety and pleasure. (sucrose increases pleasure) (fat helps satiety)

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5
Q

To taste, we need to ____ the stimuli

A

hydrate (stimulated saliva is in prep of food)

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6
Q

Saliva is made of

A
  • 99% Water
    – Electrolytes
  • Eg - Sodium, potassium, calcium chloride, magnesium,
    bicarbonate, phosphate
    Proteins
  • Enzymes such as amylase
  • Immunoglobulins and other antimicrobial
  • Mucosal glycoproteins
    – Glucose and nitrogenous products (urea, ammonia)
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7
Q

Our saliva has adapted to ___

A

Salt/ saltiness

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8
Q

What are the three parts of the major salivary glands

A

1= Parotid (largest behind mouth)
2= Submandible (under tongue behind partoid)
3 = Sublingual (under tongue)

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9
Q

5 or 6 primary basic tastes are?

A

Sweet
– Sour
– Salt
– Bitter
– Umami (savoury) (produced due to umami)
– Fat?

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10
Q

What does it mean to be a primary taste (5 points)

A

1) Has ecological consequence - needs to affect living being.
2) Is elicited by a distinctive. class of chemicals.
3) Stems from activation of specialized receptors.
4) Is detected through gustatory nerves and is
processed in taste centers.
- need neuro response
5) Evokes a behavioural and/or physiological response.
i.e sugar leads to metabolic response

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11
Q

Anatomy of the taste system

A

Need Taste receptor cells (TRC’s)
* Found in taste buds and located in papillae
* We have 4 types of papillae
– 3 required for taste perception- (fungiform,
vallate, foliate)
– 1 (filiform) is not required for taste is used to perceive the placement of food on the tongue,

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12
Q

Taste bud is similar to

A

A bulb of garlic where each clove is each tase receptor cell and they are in papillae the red dots on the tongue

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13
Q

Where are taste receptors located?

A

Tongue (in taste buds)
without tastebuds just plain cells:
* Hard and soft palate
* Airways
* Stomach
* Intestine
* Cells in the bladder
* The brain
* Reproductive tract

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14
Q

Main parts of tongue and mouth

A

Soft palate - on top
Pharynx
Foliate papillae
fungiform papillae (most)
Vallate papillae

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15
Q

Study lecture 2 slide 11 and 12 diagram

A

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16
Q

Type I TRCs percieve

A

Low salt conc

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17
Q

Type II TRCs

A

Sweet, Umami, Bitter and Kokumi

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18
Q

Type III TRCs

A

Sour, acids

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19
Q

Potential ligands for taste
perception Sweet

A

sugars, artificial sweeteners, D-amino acids, sweet proteins

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20
Q

Potential ligands for taste
perception Savoury (umami)

A

L-amino acids, glutamic acid, glutamates, aspartates
(Beefy, brothy, salty taste)

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21
Q

Potential ligands for taste
perception Bitter

A

alkaloids (such as caffeine), flavonoids (such as naringenin)

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22
Q

Potential ligands for taste
perception Salty

A

salts such as sodium, potassium, lithium

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23
Q

Potential ligands for taste
perception Sour

A

acids (generate H+ ions in solution)

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24
Q

Whats a ligand?

A

A ligand is a molecule that binds to a receptor to send signals between
cells

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25
Sweet, umami, bitter * Requires _____ __ _____ coupled receptors (GPCR
Type II G-Protein Taste 1 receptor family (TAS1) – Taste 2 receptor family TAS2) – Sweet: 2 subtypes of type 1 - TAS1R2, TAS1R3 – Umami: 2 subtypes of type 1- TAS1R1, TAS1R3 – Bitter: 25 subtypes of type 2
26
Sweet, umami, bitter.... Ligand (tastant) binds to the receptors (TAS1R or TAS2R) and then what happens next?
This activates G-protein coupled receptor (GPCR) which initiates release of gustducin. which causes various compounds to release and thus releases ATP which then activates receptors on nerve fibres that leads to taste perception
27
Salt taste requires ___ and ___
Cation and anion Sodium chloride is the salt taste we recognize as salty
28
For salt taste perception
- An influx of cations (Na+) through sodium channels in taste cell membranes – Anions (Cl-) modulate the taste quality – The larger the anion (eg acetate vs chloride), the lower the saltiness perception
29
Sour taste
Strong acids (compounds with an H+) enter taste cells through a proton-elective ion channel – Cause cell depolarization and neurotransmitter release
30
Olegustus is
Fat taste
31
Fat taste
Ligand – long chain fatty acids – Eg - linoleic acid and oleic acid * Challenges understanding fat taste because Fat has a smell – need to wear nose clips so odour isn’t a factor – Fat has a texture – made in emulsions so that texture isn’t a factor
32
problems if you can’t taste could be ?
Ageusia – “bitter blindness” is most common – Worst is taste blindness for all tastes * Could be: – Hypoageusia – Hyperageusia * Dysgeusia/Parageusia
33
Olfaction
Smell Very powerful and sensitive sense – 10,000 different compounds can be differentiated based on aroma * Strong memory link – Tip of the tongue phenomena
34
Why is smell important?
Warning, Attraction, It Evokes strong emotions due to previous experience
35
Perception from smell
Odour compounds enter the nose – Retronasally – Orthonasally * Make contact with the olfactory epithelium * In the epithelium are neurons and receptors on cilia embedded in mucus * Receptors have specifically shaped proteins that bind with specific odour molecules * Leads to neuronal activation
36
How many olfactory receptors does the nose contain
Aproximately 400 and all 400 receptor responds to several odorants – Each receptor is encoded by a different gene – Every person has a different genetic makeup for odour sensitivity (unless you have an identical twin)
37
Odour impairments:
Anosmia (cold, mucus build up) * Hyposmia - high * Hyperosmia - low * Parosmia - altered
38
Chemesthesis
Burn Sensitivity to direct chemical stimulation of touch, pain and thermal receptors in the skin and mucous membranes Could be: * Irritation, pungency, cooling, warmth or burning * In mouth chemesthetic perception is due to stimulation of the trigeminal nerve. Large differences in eating enjoyment of spicy food among the population – Desensitization – a reduction/loss in sensitivity to a chemical irritant – Due to frequent exposure to the irritant – Desensitization occurs after the stimulation has stopped and can last for up to a day – People who eat a lot of spicy food appear to build up tolerances over time – probably due to the amount of spicy food they eat
39
Large differences in eating enjoyment of spicy food among the population
- Desensitization – a reduction/loss in sensitivity to a chemical irritant – Due to frequent exposure to the irritant – Desensitization occurs after the stimulation has stopped and can last for up to a day – People who eat a lot of spicy food appear to build up tolerances over time – probably due to the amount of spicy food they eat
40
Flavour
Need to combine all sensory processes together – Odour + Taste + Chemesthesis * Odour is a significant contributor – Precisely how much is not clear
41
Sources of taste variability (4 main points)
- Individual variation Within an individual – time of day/interactions with other foods (demographics) - Experimental protocols Amount of oral cavity exposed Training of subjects - Choice of compound like sucrose vs aspartame or citric vs tartaric acid - method of rating Magnitude estimation vs visual analogue scale vs labelled magnitude scale
42
Genetics in taste testing
genetic testing The bitter receptor gene Homozygous - AA,PP or Heterozygous - PA sensory testing - Test sensitivity to PROP or PTC
43
Supertasters
- More fungiform papillae that are larger than a regular tasters tongue - Have PP or PA gene - Experience more chemical irritation (burn is strong) - Typically smoke and drink less than non-tasters - Bitterness is strong Fischer things that supertasters tend to be tall and lean while non are soft, round and fat
44
Age when it comes to taste olfaction
Genetic influences are modified by age (kids prefer carbonated drinks over milk or water when they have that bitter receptor genotype If you have the bitter receptor genotype, as you age, you seem to lose ability to detect bitter
45
Familiarity in taste ofalction
Exposure to different flavours (breastmilk, amniotic fluid) may explain individual differences in food acceptability later in life
46
Smoking effects on taste
Female smokers – higher sucrose thresholds Maybe because: * Poor oral hygiene * Nicotine inhibits gustatory neural pathways * Maybe it’s not related to smoking but related to other factors * PROP sensitivity – smokers are more likely to be PROP sensitive therefore different in sucrose thresholds No clear answer but we know it does impact
47
Smoking significantly changes the taste perception for
Sweet, salt, and bitter taste also impact their olfaction
48
Vape liquid with taste olfaction
People who did not vape liked vanillin odour more than those who vaped Maybe due to sensory specific satiety for people who vape (vape users typically choose sweet vape flavours)
49
Is taste impaired if you have diabetes?
Yes, a test was done, testing for PROP, quinine, citric acid, sucrose and sodium chloride infused on filter paper, and DM2 patients showed identification impairment in salt taste and smell recognition. Those who were impaired showed higher fasting blood glucose levels (suggesting a possible relationship between glycemic levels and impairment in smell and taste.
50
Does sensory perception change if you exercise?
Sweet taste intensity, sensitivity, and preference were increased by acute physical exercise, but sweet preference was reduced by chronic physical activity. Salty taste - perceived intensity and sensitivity decreased but an increased preference was noted during/following exercise. Sour taste intensity ratings were decreased following exercise and preference was enhanced. Umami taste intensity and sensitivity increased following exercise and preference was decreased. No significant results were obtained for bitter taste.
51
What about flavour changing for athletes?
Running affects nasal air flow and saliva because they increase in the beginning then decrease causing perception change. Sweet, fruit sweet, floral and fresh decrease over time and sour, salty and astringent increase over time
52
Flavour wheels are used for
describing food and its tastes/flavours used for beer, wine, olive oil, and bottled water.
53
What are the functional properties of taste and smell?
Adaptation which is common in odours and tastes causes a decrease in responsiveness under conditions of constant stimulation Mixture interactions (occur with both tastes and odours) this can be due to suppression and enhancement
54
Taste-Taste interactions
Oral physiological interactions and cognitive interactions
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Oral Physiological interactions
One compound interferes with the receptor cells or with taste transduction mechanism of another compound * Example - sodium salts and bitterness or sucrose and sodium chloride Like adding sugar to watermelon to bring out sweetness
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Cognitive interactions
* Mixture suppression - when two or more taste stimuli are mixed together, the perceived intensity is less than the sum of the individual taste intensities * Example – sweet and bitter Like adding sugar to bitter coffee
57
What if I have two tastes in a solution?
This is called a binary solution and there's a low medium and high intensity which can be impacted and Taste perception in binary solutions varies depending on intensity
57
When do you perceive textures?
1. Visual 2. Initial ingestion – first bite 3. Incision and repeated chewing – mastication 4. Swallowing 5. After “mouthfeel”
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Types of textures
* Visual * Auditory * Tactile
59
Two types of mechanisms for hearing
* Air conduction * Bone conduction (fizz of soda)
60
Tactile textures: What textures can you“feel”? Chemically induced
* Astringency * Cooling * Burning
61
Tactile textures: What textures can you“feel”? Product rheology
* Creaminess * Viscosity * Sticky
62
Astringency
Dry puckering-like sensation in the mouth due to shrinking, drawing or puckering of the oral epithelium following consumption of polyphenolic compounds * Due to aggregation and precipitation of salivary proteins (mucins) when interacting with polyphenols * Polyphenols are found in: * Plant based products * Tea (tannins) * Whey protein * Red wine * Alum
63
Cooling
* Due to the trigeminal system * Most well known compound for cooling is menthol * Activates both the olfactory region (it smells minty) and the trigeminal system (it is cooling and may also be painful) * When combined with xylitol you have sweetness and cooling (they are often together in chewing gum
64
Burning
* Also due to the trigeminal system * The most common compound is capsaicin
65
zczesniak (1963)
Texture is a sensory property that is based on food structure * Manifests itself with applied force * Forms the basis of the texture profile analysis (TPA) * Geometrical properties: * Size, shape orientation of particles * Mechanical properties: * Response to applied force * “Other” properties * Fat and moisture
66
texture analysis testing done using
texture profile analysis instrument 2- bite compression tested (most famous) tests how food breaks in mouth People use figure 8 when chewing and jaw comes up the machine goes down and uses same pressure every time which humans don't do
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Textural properties extracted from the force time curve PRIMARY CHARACTERISTICS
* Hardness * Cohesiveness - sticks to itself * Viscosity * Springiness * Adhesiveness - sticks to you
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Textural properties extracted from the force time curve SECONDARY CHARACTERISTICS
* Chewy * (hard x cohesive x springy) * Gummy * (hard x cohesive
69
What are the 7 errors in sensory testing set up?
1. Stimulus error 2. Logical error 3. Expectation error 4. Error of habituation 5. Halo effect 6. Order of presentation of samples 7. Mutual suggestion
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Stimulus error
Irrelevant criteria are used to assist with the evaluation (ie, packaging) Minimize by: * Blind tasting * Avoid external cues (nothing impacting the evaluation)
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Logical error
When 2 or more characteristics of the sample are associated in the minds of the panelist Minimize by: Masking any differences – serve in coloured glasses, serve under red light
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* Expectation error (error of anticipation and Error of habituation
Expectation error : Panelists expect what they are looking for (know what to look for) Error of habituation: You habituate your response (goes down) Minimize by Providing no details about samples Randomizing samples mixed ingredient lists
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What is the halo and horns effect in testing?
Halo effect – One positive attribute affects all others (if something is bad then everything is bad about that product) “Horns” effect – If a negative characteristic is evaluated, it will influence other attributes in a negative direction
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How to minimize the halo and horns effect in testing?
– Pretesting the number of questions asked – Make sure you use the correct type of panel for the evaluation of products
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What is the problem with order of presentation of sample
Biases that may occur * Idiosyncratic scale use * Error of central tendency * Contrast effect (comparing and making decisions based on first sample)
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Mutual suggestion and how to minimize
Panelists respond to others around them Minimize by: * Separate sensory testing booths * Discouraging facial expressions and vocalizing of opinions
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To prevent errors in sensory testing (5 things)
– Labelled with a 3-digit code – Randomized during presentation – All packaging/identifiers removed – Served under red light if required – Tested in sensory booths with people distanced from others
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Sample presentation Randomization vs counterbalancing
* Randomization – samples are evaluated by each person in a random order (not as good as cb) * Counterbalanced – each serving sequence occurs an equal number of times during testing
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Completely randomized designs and Randomized complete block designs
Completely randomized designs (CRD) * Samples are randomized across subjects – Randomized complete block designs (RCBD) * Samples are randomized across subjects within a block (every person sees each sample but only once, used for trained samples)
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Essentials for testing in sensory (environment)
– Waiting area/room – Neutral colours – Positive air pressure (no smell in booth) – Temp of 20-22C, 50-55% humidity – Standardized illumination
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Why is a simple difference test important?
- You want to make a healthier product but not lose customers. - You want to change ingredients (maybe you found a cheaper supplier) and you don’t want people to notice a difference. - You want to change production lines. - You want to change packaging.
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An overall difference test tells you?
simple difference – Only tells you if there is a difference, not what the difference is
83
An attribute difference test is?
directional difference – Does the product differ in X? which is sweeter, which is more lemony??
84
What is the triangle test?
Simple difference test You present 3 samples where 2 are of the same products and one is a different product and you need to find which one is different. (would test around 60-70 ppl) 6 orders randomly presented to panelists like (aba, aab, abb, bab, bba, baa)
85
What is a duo-trio test?
Simple difference test There are 3 samples and 1 same is a reference and then 2 test samples. The panellist has to match one test sample to the reference sample. 1st way to do this is always to use 1 sample as a reference making a constant ref test 2nd way is to use both samples as references making balanced reference test samples 1 and 2 always randomized
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The most basic difference tests are
triangle duo and trio
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What is the Dual standard test?
Simple difference test It's a duo trio with two references so two references and two samples making the probability of guessing 50 % (matching test
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What is the tetrad test?
Simple difference test Two samples that don't have obvious differences is useful when product differences are small. each panellist get 4 samples and you have to group them into two groups count a number of correct responses and find statistical differences
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A/Not A test is what
Simple difference test “gold standard” product that you want to compare other samples. - Before the session, familiarize panellists with sample “A” and sample “not A” - Panelists are presented only one sample at a time
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What is a Simple difference paired comparison (also called a same/different test
Simple difference test Panelsts get 2 samples and have to indicate if they are different, useful when they are strongly flavoured.
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what is a DIRECTIONAL DIFFERENCE TEST?
attribute test Find difference between samples and how intense the difference Ie, which is sweeter analysis involves Conducting an ANOVA to determine if there is a significant difference between the samples
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What is a PAIRED COMPARISON TEST (also known as a 2AFC)
attribute test How do two samples differ in a specific characteristic? – For determining attribute differences between two samples just two samples and circle what is sweeter
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Whats a 3- AFC test ?
attribute test Three samples and given as a triangle test but they determine specific attributes and only differ in that attribute ie, which is sweeter
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Thresholds
Everyone can smell and taste at a certain level. if she sprays something some can pick it up others cant same for taste. Below a certain threshold people can't detect or taste and above a certain one people can
95
Thresholds terminology (Detection, recognition, terminal, difference)
Detection (also known as absolute): Below this point, there is no sensation and above this point you perceive something * Recognition (important) * Terminal: point at which perception does not increase minimum amount needed to notice diff * Difference: the minimal amount needed to produce a noticeable increase in the sensation
96
Types of thresholds tested for are?
* Aromas * Tastes * Flavours * In: – Water – Oil – Wine – Whatever you are interested in studying
97
Why test thresholds?
To determine how groups of individuals perceive different odours/flavours/tastes – Health – Age – Gender * To compare to instrumental assessments * To measure taints in foods * To identify the compounds that are most contributing to a flavour
98
Methods for determining thresholds are
1. Method of limits (method of least noticeable difference, method of minimal changes, method of serial exploration) 2. Method of average error (method of adjustment)
99
If series is ascending, error is?
Error of anticipation
100
If series is descending error is?
– Fatigue/adaptation occurs – Error of habituation can also occur
101
To remove the individual errors:
have 2 (2AFC) or 3 (3AFC)
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What are the types of data collected?
* Categorical – Nominal – Ordinal * Continuous – Interval – Ratio
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Categorical – Nominal
Observations fall into groups – Groups are NOT related – independent – You are identifying or classifying an object – Data is qualitative – Example: Question: Are you happy today? Response options: Yes, No, I don’t know
104
Analysis of nominal data
Count the frequency of responses. * The easiest way to analyze the frequencies is to conduct at χ2 analysis. * This will tell you if there are significant differences between the groups. * After that the analysis can sometimes get complicated
105
Categorical * Ordinal
– Observations fall into groups * Can identify the “order” that something appears * But can’t tell the magnitude of the difference * Data is quantitative
106
Advantages of ordinal scales
Used with one or more products * Can be used for intensity and like/dislike responses * Simple * well suited to consumers * Speed and accuracy of data coding and tabulation (when data tabulated manually
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Disadvantages
* You only know which sample is more (or less) of an attribute * You do not know anything about attribute intensity
108
Continuous data * Can be collected using
either interval or ratio scales * Observations that are measured along a continuum * These scales provide the most comprehensive information of all scales used in sensory testing * You get an understanding of the order of the differences as well as the magnitude of the differences
109
Advantages of category scale involve
Used with one or more products * Can be used for intensity and like/dislike responses * Relatively simple for people to understand how to use
110
Disadvantages of category scales
The number of alternative responses is limited. * Labels or integers may be biased to subjects. * Tendency to distribute their judgments along most of the scale range or avoid the end categories.
111
Advantages and disadvantages of ratio scaling
Advantages * Panelists create their own scale * No endpoint bias * Can be used for intensity and like/dislike responses Disadvantages * The data needs to be normalized to get rid of outliers * Hard to explain to panellists * Cannot be used for children
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What is most commonly used for trained panel and consumer
trained panel has line scale consumer uses hedonic scale
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