Midterm reviewer

Question 0

Initial absorbance read from the spectrophotometer was 0.803. After 10 minutes, absorbance reading was 0.773.

Find the percentage dye reduced

Answer 0

96.26%

Question 1

What condition in the experimental set-up as described in the result?

Result: Slower rate of Transport or may inhibit totally

Answer 1

1:2 ringer:NaCN (IN) – 1:1 ringer:NaCN (out)

Question 2

Two Factors affecting the separation of pigments from each other in the stationary phase during color development in chromatography

Answer 2

Polarity of pigments

Solubility of the pigment in solvent used

Question 3

Which particular structure in chloroplast does the light reaction take place in photosynthesis?

Answer 3

Thylakoid

Question 4

Which particular structure in chloroplast does the dark reaction take place in photosynthesis?

Answer 4

Stroma

Question 5

Strips of filter papers used in chromatography procedure is in what phase?

Answer 5

Stationary

Question 6

9:1 petroleum ether solution used in chromatography procedure is in what phase?

Answer 6

Mobile

Question 7

Chlorophyll a in Photosystem I absorbs light with a wavelength of 700 nm and is also known as _____.

Answer 7

P700

Question 8

Na + - K+ pump

-passive transport
or -active transport

Answer 8

Active transport

Question 9

_____ states that the absorption of light by a substance is proportional to its concentration in solution.

Answer 9

Beer-Lambert Law

Question 10

Which molecule in the electron transport chain during the Hill reaction in photosynthesis does DCPIP replaces as an artificial electron acceptor in the experiment? _____

Answer 10

NADP

Question 11

Facilitated diffusion
-passive transport
or -active transport

Answer 11

Passive

Question 12

Give the condition in the experimental set-up as described in the result.
Result: Water moves in as Na+ enters the cell

Answer 12

Ringer (IN) – Ringer(OUT)

Question 13

_____ was the substance used in the experiment which inhibited the reaction when chloroplasts are exposed to light.

Answer 13

DCMU

Question 14

Give the condition in the experimental set-up as described in the result.
Result: . No net change on the rate of transport

Answer 14

Empty (IN) – Ringer (OUT)

Question 15

In vivo, which 2 molecules does light energy become converted into during the light reaction phase? _____ and _____

Answer 15

NADPH

ATP

Question 16

_____ is the wavelength at which the absorption reading exhibits the highest value.

Answer 16

Absorption maximum of a certain substance

Question 17

When the same color in the strips were cut and grouped together, placed in a tube containing acetone, shaken for 5-10 minutes, the product is a partially purified pigment. This procedure is called _____.

Answer 17

Elution of pigments

Question 18

Osmosis

-passive transport
or -active transport

Answer 18

Passive