MIDTERM_LABORATORY Flashcards
(61 cards)
1
Q
Best for detecting poxviruses
A
CYTOLOGY & HISTOLOGY
2
Q
- OWL EYE FORMATION
- Syncytial formation wherein two cells combine
A
CYTOMEGALOVIRUS
3
Q
PAPILLOMA VIRUS stained with H&E:
A
KOILOCYTES
4
Q
- Simple and cheap test that relies on viewing and interpretation of a single cell from the sample of aspirated vesicle lesion
A
TSANCK SMEAR TEST
5
Q
- used to distinguish various blistering usually Varicella-Zoster Virus (VZV) and herpes
simplex virus (HSV) type I
A
TSANCK SMEAR TEST
6
Q
- inclusion body considered to be the
the infectious virion of rabies
A
NEGRI BODIES
7
Q
- greater magnification and can be used to
detect virions
A
ELECTRON MICROSCOPY
8
Q
NON CULTURABLE VIRUSES:
A
NORWALK
ROTAVIRUS
ENTEROVIRUS
ADENOVIRUS
9
Q
■ distinctive and characteristic visual changes in infected cells produced by viruses
A
CYTOPATHIC EFFECTS
10
Q
- Detect RESPIRATORY SYNCYTIAL VIRUS and INFLUENZA A VIRUS from respiratory specimens
A
ENZYME IMMUNOASSAYS
10
Q
○ Can be a valuable tool to detect various viral
agents directly in clinical specimens
A
IMMUNOFLUORESCENCE MICROSCOPY
11
Q
Allows the direct visualization of virus
A
FLOURESCEIN ISOTHIOCYANATE
12
Q
- UNLABELED ANTIBODY is added to the slide, followed by a labeled FITC
- Used when lower quantities of virus
A
INDIRECT IMMUNOFLUORESCENCE
13
Q
- USES LABELED ANTIVIRAL ANTIBODY (fluorescein isothiocyanate)
- Rapid, specific, less sensitive
- For large quantities of virus are suspected
A
DIRECT IMMUNOFLUORESCENCE
14
Q
VIRAL ISOLATION 3 METHODS:
A
cell culture
animal inoculation
embryonated egg
14
Q
A thermostable DNA POLYMERASE
A
Taq Pol
15
Q
- Used only in REFERENCE LABORATORY = RITM
A
ANIMAL INOCULATION
16
Q
PRIMARY PURPOSE:
- Isolate and identify viruses in clinical samples
- prepare viruses for vaccine production
A
VIRAL ISOLATION
17
Q
Visible clumping of RBC
A
HEMAGGLUTINATION ASSAY
18
Q
able to hemadsorb and hemagglutinate guineapig RBC
A
INFLUENZA A & B
19
Q
TISSUE CULTURE was first applied in diagnostic virology by?
A
STEINHARDT & COLLEAGUES (1913)
20
Q
- used cut tissues in nutrient media for cultivation of vaccine viruses
A
MAITLAND
21
Q
First to culture POLIOVIRUS in tissue culture of nonneural origin
A
ENDERS, WELLER & ROBINS
22
Q
○ Three Types of TISSUE CULTURE
A
■ Organ Culture
■ Explant Culture
■ Cell Culture (commonly used)
23
Cell cultures are monitored for virus growth by
microscopic inspection for up to __ weeks.
4
24
Phenol red, a pH indicator remains ____ at
physiologic pH, _______ at acidic pH, and ______ at
alkaline pH, is added to monitor the pH of the
medium.
RED, YELLOW, PURPLE
25
Varicella and measles assays
- past infection or immunization (present IgG)
■ implies that congenital infection will not occur
during subsequent pregnancies
POSITIVE
26
○ useful for organ transplant donors and recipients (premature hospitalized babies)
CMV IMMUNE STATUS
27
➢ It's a serum-rich nutrient medium designed to support rapid cell growth.
➢ Used to initiate the growth of cells when cell
cultures are prepared in-house or to feed
purchased cell cultures
GROWTH MEDIUM
28
➢ to determine if patients have been infected with
(or vaccinated for) a virus
IMMUNE STATUS TESTING
29
most commonly for assessing the immune status in
health care workers
VARICELLA AND MEASLES ASSAY
30
refers to the removal of old
medium, followed by the addition of fresh culture
medium.
FEEDING
31
➢ Like a growth medium, it supports cell metabolism.
➢ Contains less serum (0%-2%) compared to
growth medium.
➢ Keeps cells in a steady state of metabolism.
➢ Uses fetal, newborn, or agammaglobulinemic
MAINTENANCE MEDIUM
32
○ MAINTENANCE MEDIUM is classified into 3 different types based on their;
■ Origin-Primary Cell Line
■ Chromosomal Characteristics-Diploid Cell Lines
■ Number of Generation-Continuous cell lines
33
periodically removing
cells from the surface, diluting them and placing
them into a new container.
SPLITTING/PASSAGING
33
● Obtained from tissue removed from an animal
PRIMARY CELL CULTURE
34
Criteria for diagnosing primary reinfection:
PRESENCE OF IGM
SEROCONVERSION
35
● Complement Fixation Test
● ELISA
● Indirect Immunofluorescence
● Western Blotting
VIROLOGY TESTS
36
● Low passage cell lines/Finite cell lines
● Must have at least 75% of cells with same karyotype as the normal cells which they are derived
DIPLOID CELL LIINE
37
EX. OF CPE
- MOLLUSCUM CONTAGIOSUM
HANDERSON PETTERSON
38
○ standard finite cell culture used in diagnostic virology
HUMAN NEONATAL LUNG
39
● Special technique for cell identification
● rapidly identify viruses than the traditional cell
culture method
SHELL VIAL CENTRIFUGATION
40
Typical Serologic Profile after acute infection:
● Fourfold or more increase in titer of IgG.
● Total antibody between acute and convalescent
sera.
● Absence or slight decrease in IgM.
41
● Infinite cell line
● capable of infinite passage which causes your
cell to have a malignancy
CONTINUOUS CELL LINE`
42
Heteroploid or immortal cell lines:
■ HeLa; derived from human cervical carcinoma
■ HEp-2; derived from carcinoma of the human larynx
■ KB; derived from nasopharyngeal carcinoma
■ A549; derived from human lung
carcinoma
■ Vero; derived from AGMK; monkey
kidney
43
Heteroploid or immortal cell lines:
- derived from human cervical carcinoma
HeLa
44
Heteroploid or immortal cell lines:
- derived from carcinoma of the human larynx
HEp-2
45
Heteroploid or immortal cell lines:
- derived from nasopharyngeal carcinoma
KB
45
Heteroploid or immortal cell lines:
- derived from human lung
carcinoma
A549
46
● lines of cells that contain a mixture of two
different cell types
MIXED OR ENGINEERED CELL CULTURES
47
Heteroploid or immortal cell lines:
- derived from AGMK; monkey kidney
VERO
48
● Presence of virus indicated by areas of dead or dying cells
CYTOPATHIC EFFECTS
49
EX. OF CPE
- SMALL POX
GUARNERI
50
EX. OF CPE
- MEASLE
WARTHIN FINKELDY
51
● produces an HSV-like CPE
● grows much more slowly and only on diploid
fibroblasts.
CYTOMEGALOVIRUS
52
EX. OF CPE
- HSV
HSV - Cowdry type A bodies in tzanck smear,
large, rounded cell
53
EX. OF CPE:
RABIES
NEGRI BODIES
54
EX. OF CPE:
HPV
KOILOCYTES
55
EX. OF CPE:
RSV
CLASSICAL SYNCYTIAL FORMATION
56
ex. OF CPE:
CMV
OWL EYE APPEARANCE
57
EX. OF CPE:
ENTEROVIRUS
Refractile, round cells in
cluster
