Mitosis and Meiosis Flashcards

1
Q

In what types of cells can mitosis occur?

A

In all. Mitosis will only give identical cells, so it does not matter if they are haploid, diploid or etc.

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2
Q

Why do cells undergo mitosis?

A

The cell needs to create daughter cells to cope with lost ones (repair), create new ones for growth, to produce new organisms (asexual way of reproduction),. Mitosis is also implicated in the process of immunity. The process of aging also arises from mitosis

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3
Q

Give an example of an uncontrolled mitosis.

A

Cancer. The checkpoints do not work well and growth is thus uncontrollable.

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4
Q

Would you expect an adult or a child to do more mitosis? Why?

A

Children. They grow a lot while adult do not grow anymore. Furthermore, some old people seem like they are shrinking.

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5
Q

What are the stages of mitosis in order?

A

Prophase, prometaphase, metaphase, anaphase, telophase

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6
Q

What steps are in the cell cycle but not in the mitosis process?

A

Interphase (G1, S-Phase, G2). Cytokinesis can not be quite included here because it starts in late anaphase or early telophase.

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7
Q

In what phase of the cell cycle does the cell pass the most of its life?

A

In the G1 process because a lot of metabolic activity occurs in here before going on with the big move of mitosis.

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8
Q

What would happen if SPF was produced without it being ready?

A

SPF comes after G1 when the size, the growth rates and the nutrients in the cell are adequate. The S-phase is thus initiated. In this case, the cell would not have enough nutrients to replicate DNA and some genes might be missing due to an uncompleted gene expression during protein synthesis. DNA would replicate without the cell being ready for it.

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9
Q

What happens to the chromosomes during the S-phase?

A

They go from ss to ds as DNA replicates.

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10
Q

What does S stand for in S-phase?

A

Synthesis

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11
Q

What would happen if APC was not produced?

A

APC comes after metaphase to launch anaphase. No anaphase means that sister chromatids could not be separated at the centromere to go in their daughter cell. Thus, The daughter cell would have double-stranded chromosomes and twice the ploidy level of the mother cell because it could not divide into two daughter cells. Without creating a segregation in the chromosomes due to spindle fibers contracting, two regions of the cells are not created and thus the daughter cells are not.

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12
Q

What happens in G2?

A

The cell prepares for mitosis as it is the period just between S-Phase and the prophase of mitosis. The cell stores enough energy, synthesizes the last proteins that are needed for the big move and duplicate organelles.

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13
Q

What happens when G2 is ok?

A

When all the energy required for mitosis and all the organelles have been duplicated, the MPF is produced. It is the mitosis-promoting factor.

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14
Q

How do G1 and G2 differ?

A

G1 is for metabolic activities in the cell cycle whereas G2 really is before mitosis and stands as a preparation for this process. G1 is longer than G2. G2 has ds chromosomes while G1 has ss chromosomes.

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15
Q

What do G1 and G2 have in common?

A

They are not part of mitosis and they undertake growth in the cell.

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16
Q

How do we call the period of the cell cycle where G1, G2, G0 and S-phase occur?

A

The interphase

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17
Q

Of what proteins are kinetochores made?

A

Cohesin proteins

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18
Q

Why is the nucleolus the last part of the cell that is turned off before mitosis and the breakage of the nucleus?

A

It is in this organelle that rRNA is synthesized, so it needs to be the last gene to be turned off because it would interfere with all the process of mitosis. Ribosomes would not be able to function and DNA could not replicate.

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19
Q

What is the distinction between a spindle fiber and a microtubule?

A

A microtubule is an organelle that can make many elements, like the spindle fibers. Microtubules are the building blocks of spindle fibers. Microtubules are contained in the centrosomes, which are locations to control the microtubules of the cell. In the center of the centrosome, there are centrioles.

20
Q

What happens if the cell cycle is stopped? Is it an exception?

A

The cells go in the G0 phase. It is the fate of most cells. Mitosis is suppressed for these cells and they will thus die sadly.

21
Q

How does G0 happen?

A

It happens if SPF is not produced. In this case, DNA will never come to replicate and the cell will thus be left undivided until it dies.

22
Q

What is a cancer?

A

It is a cell that has lost its controls like APC, SPF, MPF. Thus, it goes into uncontrollable division. Cancers do not listen to SPF.

23
Q

What types of proteins are SPF, APC, MPF?

A

They are cyclins because they are control proteins that regulate the cell cycle.

24
Q

What happens in animals at cytokinesis? When does it start?

A

It superposes mitosis in that it starts during early anaphase. In this case, the spindle fibers will go in the center of the daughter cells by pulling on the microtubules of the opposite pole. In the center, a contractile ring of actin filaments will thus be formed and pinch the mother cell into two daughter cells. Thus, the ring constricts and is called a cleavage furrow. This does not happen in plants.

25
Q

What happens in plants at cytokinesis?

A

There is a cell plate that forms, which is the start of the cell wall. The cell plate forms by the fusion of vesicles that come from the Golgi complex.

26
Q

Why does cytokinesis differ in plants and in animals?

A

It needs to because the cell’s are not built in the same way. Plants have cell walls and animals do not. Thus, there needs to be a cell plate for plants whereas the cleavage furrow suffices for animals.

27
Q

At what step of mitosis does the number of chromosomes increase?

A

In the anaphase. When the sister chromatids separate, they are ss chromosomes on their own. The cell is thus briefly twice the ploidy level just before daughter cells are created.

28
Q

Give an example of mitosis that is not followed by cytokinesis.

A

Seed development.

29
Q

How does DNA replicate?

A

When SPF is produced, the S-phase starts. The two strands of the DNA molecule are separated and new nucleotides are added to the separated ones to match the purines and pryrimidines.

30
Q

Why is MPF necessary to mitosis?

A

It initiates the process. First, it allows chromatin to condense in chromosomes. The nuclear membrane is also broken down in the prometaphase and the mitotic spindles are assembled. If there is no MPF, chromosomes would not condense and it would be likely that some parts are lost because its hinders separation. Not breaking the nuclear membrane involves that the chromosomes cannot be brought to two poles of a cell to make identical daughter cells. No mitotic spindles makes it harder for the cell to create two regions as these structures need to push on the poles to enlarge it. Also, they could not carry the chromosomes to their region.

31
Q

What checkpoint activates APC? What does it wait for?

A

The mitotic spindle checkpoint. The cell waits for all kinetochores to be attached to each chromosomes. Or else some would be lost in the process.

32
Q

Why is APC absolutely required?

A

It is needed for chromosomes to separate.

33
Q

In what organisms do we find centrioles?

A

In animals only.

34
Q

Above from allowing the chromatids to separate, what does APC allow?

A

It inactivates the MPF for the mitosis process to be undone. This way, the chromosomes will de-condense and spindle fibers will break down. It informs that anaphase is finished.

35
Q

What are non-sister chromatids?

A

They are chromatids from homologous chromosomes, but not on the same chromosomes.

36
Q

How can non-sister chromatids be different?

A

They have different alleles.

37
Q

What happens in meiosis I that is different from mitosis?

A

Instead of being ds chromosomes that group at the center, it is synapses that separate. Synapses are tight pairs of homologous chromosomes that are held by proteins. Also, crossing-over occurs where there is exchange of DNA between homologous pairs. Chromosomes arrangement also differ.

38
Q

Describe a cross-over.

A

It is a part of DNA on a chromosome that was exchanged from another homologous one. Cohesin proteins are removed from centromere after crossing-over, which occurs in prophase I. The pairs are now held at the chiasmata. Crossing-over in genetic recombination. The points where the crossover occurred are called recombination nodules, large protein assembles.

39
Q

In what ways can meiosis produce two different gametes?

A

In meiosis I: -Crossover of non-sister chromatids at prophase
-Random assortments of tetrads at the metaphase plate. Maternal and paternal are not all on the same side of the cell. So assortments of homologous pairs are not the same.

In meiosis II: -Anaphase II pulls sister chromatids apart. However, sister chromatids are not necessarily identical at this stage because of cross-overs. Thus, it depends on which chromatid goes on which side.

After: Random fertilization of two individuals.

40
Q

What is the difference between meiosis II and mitosis?

A

The steps are the same except that it is with haploid numbers instead of diploid, in most cases.

41
Q

What is interkinesis?

A

It is the step between meiosis I and meiosis II in which there is basically the same thing as in the interphase. However, there is no S-phase. It is only growth and preparation for meiosis II.

42
Q

How could you obtain a diploid gamete?

A

There could be a problem in anaphase I. A mitotic spindle could attach to two chromosomes that are homologous and fail to detach the tetrad (they attach to the same spindle). This way, one side receives both homologous chromosomes and the other one none. This is a nondisjunction. In meiosis II, the chromosomes will separate and have as a result two copies of a chromosome instead of one (homologous). This way, gives 2n. And fertilization will give 3n. Triploid oysters…

43
Q

What are functions of meiosis?

A

Create haploid cells, create genetic variation.

44
Q

How can meiosis create new genes combination?

A

Cross-over in prophase I.

45
Q

How can meiosis create new chromosome combinations?

A

Random segregation in metaphase I. Orientation is not the same, so maybe will get maternal w/ paternal or something else.