Mitosis & myosis Flashcards

1
Q

Parts of an egg

A

nucleus - cytoplasm- zona pellucida - plasma membrane - cumulus?

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2
Q

frog cell cycle

A

splits every 15-20 min

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3
Q

Cell theory

A

all organisms consists of cells
cells divide to produce new cells
higher organisms fuse their cells to produce a new organism

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4
Q

chromatin

A

combination of protein (especially histones) & DNA

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5
Q

chromatid

A

1 “mitotic chromosome” has 4 chromatids, connected by a centrosome

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6
Q

Steps in cell division

A

chromosome replication (s phase) – G2
checkpoint
mitosis or myosis (M) – g1
cytokinesis

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7
Q

mechanism responsible for structure of spindle

A

polar microtubules attach to each other

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8
Q

ingredients for making a metaphase mitotic spindle

A

sister chromatids remain paired until all chromatids are attached to a kinetochore microtubule
microtubules grow & shrink until captured by kinetochore
2 kinetochore microtubules from the same spindle cant capture both sister chromatids of a chromosome
checkpoint when metaphase is done (all captured & stable in middle)

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9
Q

Continuous vs discrete variation

A

height, eye color

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10
Q

independent traits

A

prob of a wrinkled yellow pea = prob of yellow * pro of wrinkled

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11
Q

mendel’s law irl

A

represents alleles of same gene on different homologues

also shows independence of traits

also think of female vs male cross chromosomes

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12
Q

Types of alleles

A

Wild type - predominant, 99%
Mutant allele - change from wild type as a result of recent mutation; also can refer to alleles that causes diseases
Polymorphic allele - contributing to the variation in population

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13
Q

not independent assortment

A

cross over
Dominant alleles linked in trans or cis
more likely of recombination for closer chromosomes - measure of distance = 100* (# recombination)/(# total progeny) in centiMorgans

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14
Q

semi-dominant trait

A

3 phenotypes for 1 gene, 2 alleles

–> in population as a whole, more than 2 - continuous traits

ex: alzheimer’s

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15
Q

multigenetic (polygenic)

A

actual phenotype is the effect of many diff genes - bell type

ex: heart attach

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16
Q

environmental contribution to phenotype

A

penetrance: percentage of individuals of a given genotype that show the phenotype at all

Expressivity: the degree to which a phenotype is expressed

17
Q

continuous traits

A

multigenic, allelic series, environmental effects

18
Q

how to purify DNA

A
  • grind up organism
  • extract lipids proteins w organic solvent (eg phenol)
  • precipitate w ethanol
19
Q

assay for genetic material

A

transforming principle - a chemical component from 1 cell s capable of genetically transforming into another cell