Mod 3 Pt. I Flashcards
3 Universal Ancestor
Domain Eukarya
Domain Archaea
Domain Bacteria
Domain Eukaryota or Eukarya; their name comes from the Greek eu__ and karyon ___
“true”
“nut” or “kernel“.
surrounded by a plasma
membrane and contains many different structures and organelles with a variety of functions.
eukaryotic cell
used to describe unicellular (single-celled) organisms that lack true nucleus and membrane-bound cell organelles. This means that the genetic material in prokaryotes is not bound within a nucleus.
Prokaryotes or procaryotes
Prokaryotes or procaryotes is coined from two Greek
words
pro, before, and karyon, nut or kernel.
(Eukaryotic cell structures & function)
- Strengthen and give shape to the cell
Cell wall and pellicle
(Eukaryotic cell structures & function)
- Photosynthesis—trapping light energy and formation of carbohydrate from CO2 and water
Chloroplasts
(Eukaryotic cell structures & function)
- Cell movement
Cilia and flagella
(Eukaryotic cell structures & function)
- Environment for other organelles, location of many metabolic processes
Cytoplasmic matrix
(Eukaryotic cell structures & function)
- Transport of materials, protein and lipid synthesis
Endoplasmic reticulum
(Eukaryotic cell structures & function)
- Packaging and secretion of materials for various purposes, lysosome formation
Golgi apparatus
(Eukaryotic cell structures & function)
- Intracellular digestion
Lysosomes
(Eukaryotic cell structures & function)
- Cell structure and movements, form the cytoskeleton
Microfilaments, intermediate
filaments, and microtubules
(Eukaryotic cell structures & function)
- Energy production through use of the tricarboxylic acid cycle, electron transport, oxidative phosphorylation, andother pathways
Mitochondria
(Eukaryotic cell structures & function)
- Ribosomal RNA synthesis, ribosome construction
Nucleolus
(Eukaryotic cell structures & function)
- Repository for genetic information, control centre for cell
Nucleus
(Eukaryotic cell structures & function)
-Mechanical cell boundary, selectively permeable barrier with transport systems, mediates cell-cell interactions and adhesion to surfaces, secretion
Plasma membrane
(Eukaryotic cell structures & function)
- Protein synthesis
Ribosomes
(Eukaryotic cell structures & function)
- Temporary storage and transport, digestion (food vacuoles), water balance (contractile vacuole)
Vacuole
(Prokaryotic cell structures and functions)
- Resistance to phagocytosis, adherence to surfaces
Capsules and slime layers
(Prokaryotic cell structures and functions)
Gives bacteria shape and protection from lysis in dilute solutions
Cell wall
(Prokaryotic cell structures and functions)
Survival under harsh environmental conditions
Endospore
(Prokaryotic cell structures and functions)
Attachment to surfaces, bacterial mating
Fimbriae and pili
(Prokaryotic cell structures and functions)
Provides the power of motility or self-propulsion
Flagella
(Prokaryotic cell structures and functions)
- Buoyancy for floating in aquatic environments.
Gas vacuole
(Prokaryotic cell structures and functions)
- Storage of carbon, phosphate, and other substances
Inclusion bodies
(Prokaryotic cell structures and functions)
- Localization of genetic material (DNA)
Nucleoid
(Prokaryotic cell structures and functions)
- Contains hydrolytic enzymes and binding proteins for nutrient processing and uptake’
Periplasmic space
(Prokaryotic cell structures and functions)
- Selectively permeable barrier, mechanical boundary of cell, nutrient and waste transport, location of many metabolic
processes (respiration, photosynthesis), detection of environmental cues for chemotaxis
Plasma membrane
- unicellular organisms without nuclei
- No organelles
- Have cell walls
- Lacks peptidoglycan
- DNA is more similar to eukaryotes, denotes lineage.
- Live in extreme environments
- No oxygen
- hot springs, deep ocean
- Energy: Chemosynthesis*
ARCHAEBACTERIA
ARCHAEBACTERIA Biological Functions:
- Protecting cell against ion & pH fluctuations, osmotic stress,
degrading enzymes or predacious bacteria. - helps in maintaining shape & envelope rigidity of the cell.
- it promotes cell adhesion to surfaces
- widely studied recently especially in connection with
nanotechnology due to their ability to self assemble protein
units without the aid of enzymes.
Major Types of Archaebacteria Cell Wall
- Type I
- The most common type of archaeal cell wall is an
S layer composed of either protein or glycoprotein.
Major Types of Archaebacteria Cell Wall
- Type 2
- Additional layers of material are present outside the S-layer. In Methanosprillum, there is a
protein sheath external to the S-layer.
Major Types of Archaebacteria Cell Wall
- Type 3, In Methanosarcina,
S-layer is covered by a chondroitin like material called as methanochondroitin.
Major Types of Archaebacteria Cell Wall
- Type 4
- S-layer is the outermost layer and is separated from the plasma membrane by
a peptidoglycan-like molecule called
psuedomurein.
Major Types of Archaebacteria Cell Wall
- Type 5
- S layer is absent.
cell wall is single thick, homogenous layer - Gram positive bacteria. These archaea often stain Gram positive. This type is present in Methanobacterium, Halococcus etc.
It is a thin layer lining the inner surface
of the cell wall.
- Semipermeable membrane controlling
the flow of metabolites - Chemically, consists of Lipoprotein and
carbohydrates. Sterols are absent
Cell Membrane
Differences between bacterial and archaeal phospholipids:
- chirality of glycerol
– the glycerol used to make archaeal phospholipids is a stereoisomer of the
glycerol used to build bacterial and eukaryotic membranes - linkage (ester vs. ether linkage)
- side chains (fatty acids vs. isoprenoid chains)
- branching of side chains
– different physical structures; can form carbon rings
embedded in or loosely attached to the cell membrane
Cell Membrane Proteins
Prokaryotic Cell membrane Functions
- permeability barrier
- regulate movement of materials into and out of cell
- contains proteins that transport nutrients into the cells
and eliminate waste materials - synthesizes cell wall components
- assist with DNA replication (anchors DNA)
- secretes proteins
- carries on cell respiration (ATP synthesis)
- contains bases of flagella
- proteins respond to chemical substances in the
environment
Transport Systems
1. Rarely used by prokaryotes; no energy expenditure (i.e.
glycerol)
2. Movement is against a concentration gradient; requires energy expenditure
- Uses proton motive force (i.e. lactose and waste products; efflux pumps)
- Uses ATP; extremely high substrate affinity of the periplasmic-binding proteins (i.e. maltose)
3. Chemically alters (phosphotransferase system) a molecule as it passes through the cell membrane; uptake does not affect the concentration gradient (i.e. glucose, mannose, fructose)
- Facilitated diffusion (Passive transport)
- Active transport
- Major facilitator superfamily
- ABC transporters - Group Translocation
used to actively move out certain proteins
synthesized by the cell
– i.e. proteins used to make up structures like
flagella; enzymes to break down substances
too large to transport into the cell
– distinguishes proteins based on characteristic
sequence of amino acids that make up one end
(signal sequence)
– uses ATP
– still poorly understood
Transport System (SECRETION)
general secretory pathway
semifluid substance inside the cell membrane
* 4/5 water + 1/5 dissolved substances (enzymes, proteins,
carbohydrates, lipids, salts, vitamins and various inorganic
ions)
* where chemical reactions take place
Cytoplasm
Colloidal system of variety of organic
and inorganic solutes in viscous watery
solution
- No ER and Mitochondria
- Contains mesosomes, inclusions, and
vacuoles
Cytoplasm
Vesicular, convoluted invaginations of the
plasma membrane
* Prominent in GM+ bacteria
* Principal sites of Respiratory enzymes
* Analogous to mitochondria in Eukaryotes
Mesosomes
chromosome region
- gel-like region containing the chromosomes and
plasmids
Nucleoid
single, circular, double-stranded
DNA molecule that contains all
genetic information required by a
cell
* 1 mm long
* 10% of cell’s total volume
– supercoiled chromosomal DNA
* Archaea chromosome: complexed with proteins; resembling histone proteins
Chromosomes
- accessory genetic information
- circular, supercoiled, double stranded DNA molecules
- 0.1 – 10% of chromosome size
- contains hundreds of genes (5-100 genes)
- can be of many types per cell
- not required but may provide bacteria
with genetic advantage - may contain genes for antibiotic resistance
(R), disease production - can be transferred and spread to other
bacterial cells - replicate independently from chromosome
Plasmids
- sites for protein synthesis
– protein – 50% of bacterial cell dry weight
– 90% of cell energy is for protein synthesis - the faster the cell is growing, the faster
proteins are produced, the greater the
number of ribosomes - site for antibiotic action (streptomycin and
tetracycline)
Ribosomes
Subunit Ribosome
Prokaryotic Ribosome
- 30S; 50S = 70S
Eukaryotic Ribosome
- 60S; 40S = 80S
contain the pigments used to capture light energy for synthesis of sugars
Chromatophores/ chlorosomes
convert nitrogen compounds into plant-useable form
– house the enzymes used in deriving energy from
oxidation of nitrogen compounds
nitrifying bacteria
- variety of small bodies within
the cytoplasm - storage of materials that are
later use as source of nutrients - reservoir of structural building
blocks - granules; vesicles
Inclusion Bodies