module 1

Question 1

3 mL of the blood is taken, and 1 mL
of the blood is in each test tubes. Start
two stopwatches as you see the blood
in the syringe. Keep the blood in the
water bath and check for clotting by
tilting each tube at intervals of 30 to
60 seconds. Tilt the tube to greater
than 90 degrees

Answer 1

Lee- White method

Question 2

NV of Lee- White method

Answer 2

blood clots firmly within 4-8

minutes

Question 3

This test is used to monitor heparin
therapy which prolongs the clotting
time.

Answer 3

Lee- White method

Question 4

The procedure is carried out at 37 deg
Celsius and the tube is tilted after the
first minute and thereafter 5-10
seconds intervals until the clot is
formed.

Answer 4

Activated coagulation time

Question 5

NV - Activated coagulation time

Answer 5

coagulation time is complete in

less than 101 seconds

Question 6

Reagents used in Prothrombin time

Answer 6

Thromboplastin and ionized calcium

Question 7

when the reagents are added
to citrated plasma, these substitute for
tissue factor activates factor X in the
presence of factor VII involving
platelets or procoagulants in the
intrinsic pathway.

Answer 7

Prothrombin time

Question 8

NV - Prothrombin time

Answer 8

10-12 sec

Question 9

Prolongation of PT when PTT is

normal, occurs only in

Answer 9

factor VII deficiency

Question 10

Prolonged PT and and PTT occurs in

Answer 10

oral anticoagulation therapy, liver disease, Vit K deficiency and def of
common pathway

Question 11

compares the local thromboplastic reagent against

international reagent

Answer 11

International normalized ratio (INR)

Question 12

A normal INR is

Answer 12

1.0.

Question 13

A normal INR is 1.0. Each increase

of 0.1 means

Answer 13

the blood is slightly

thinner (it takes longer to clot).

Question 14

INR is related to the

Answer 14

prothrombin time (PT).

Question 15

If there is serious liver disease and

cirrhosis, the liver may

Answer 15

the liver may not produce
the proper amount of proteins and
then the blood is not able to clot as it
should.

Question 16

The test is performed in a citrated

blood.

Answer 16

Activated partial thromboplastin time

Question 17

The plasma is recalcified.

Answer 17

Activated partial thromboplastin time

Question 18

enhances the speed of contact

activation

Answer 18

Kaolin

Question 19

provides a surface on
which coagulation enzyme substrate
can occur

Answer 19

Phospholipid

Question 20

NV - Activated partial thromboplastin time

Answer 20

28-40 sec

Question 21

assess the intrinsic

coagulation pathway

Answer 21

Activated partial thromboplastin time

Question 22

APTT assess the intrinsic

coagulation pathway except

Answer 22

factor VII and platelets.

Question 23

Performed exactly the same as APTT
except that an activator is not included
in the thromboplastin reagent.

Answer 23

Partial thromboplastin time

Question 24

NV - Partial thromboplastin time

Answer 24

40- 100 sec

Question 25

is a thromboplastin like | substance that activates factor X.

Answer 25

Stypven

Question 26

When this reagent is added together with platelets and CaCl2, the coagulation process has begun at the point of factor X activation.

Answer 26

Stypven

Question 27

Stypven time is used to detect

Answer 27

It is used to detect deficiencies in prothrombin, fibrinogen, factor X and factor V.

Question 28

Stypven time also called as

Answer 28

Russell’s viper venom time

Question 29

it is an enzyme that is found in the venom of the Bothrops atrox snake

Answer 29

Reptilase

Question 30

It is capable of converting fibrinogen to fibrin and is not affected by heparin.

Answer 30

Reptilase time

Question 31

A measured amount of thrombin is added to plasma. The legth of time for a fibrin clot to form is recorded as thrombin time.

Answer 31

Thrombin time

Question 32

It measure the availability of | functional fibrinogen .

Answer 32

Thrombin time

Question 33

Prolonged thrombin time is found if | fibrinogen level is

Answer 33

low (below 75- 100 mg/dl)

Question 34

Patients with fletcher factor | deficiency will have a prolonged ---------

Answer 34

APTT.

Question 35

``` If the plasma + APTT reagent mixture is incubated for 10 minutes, the prolonged APTT will be shortened to normal or almost normal if the deficiency is due to ```

Answer 35

fletcher factor

Question 36

If the plasma + APTT reagent mixture is incubated for 10 minutes, the prolonged APTT will be

Answer 36

shortened to normal or almost | normal