Module 2: RNA & Protein Flashcards by Gavin Kallabat

______ are the links between genotype and phenotype.

Proteins

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_______, the process by which DNA directs protein synthesis, includes two stages: transcription and translation.

Gene expression

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_______ is the bridge between genes and the proteins for which they code.

RNA

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Eukaryotes also have an intervening step called ________ where pre-mRNA is processed into active mRNA.

RNA processing

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What is the major nucleotide difference in DNA and RNA?

DNA = thymine

RNA = uracil

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Which of the following is the most abundant?

A. mRNA
B. rRNA
C. tRNA

B. rRNA

Most abundant = rRNA

Least abundant = mRNA

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Which of the following is the most unstable?

A. mRNA
B. rRNA
C. tRNA

A. mRNA

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Which of the following is NOT a biological role of RNA?

A. Transmission of genetic information
B. Storage of genetic information
C. Catalysis and structural components of macromolecules
D. Gene regulation
E. All of the above are biological roles of RNA

E. All of the above are biological roles of RNA

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Which of the following is polycistronic?

A. Prokaryotic mRNA
B. Eukaryotic mRNA

A. Prokaryotic mRNA

prokaryotic mRNA is polycistronic (contains information for more than one polypeptide chain)

Eukaryotic mRNA is monocistronic (contains information for only one polypeptide chain)

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______ functions in translation of genetic information from mRNA into proteins.

A. mRNA
B. rRNA
C. tRNA

C. tRNA

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Which of the following is highly structured?

A. mRNA
B. rRNA
C. tRNA

C. tRNA

tRNA contains:

Acceptor stem: where a specific amino acid is linked
Anticodon loop: base pairs with the codon on mRNA
Unique nucleotide bases

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______ is the structural component of ribosomes.

A. mRNA
B. rRNA
C. tRNA

B. rRNA

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______ is an adaptor molecule that carries a specific amino acid to the ribosome/mRNA complex.

A. mRNA
B. rRNA
C. tRNA

C. tRNA

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______ is the template for protein synthesis in eukaryotes.

A. mRNA
B. rRNA
C. tRNA

A. mRNA

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What are the steps in EUKARYOTIC transcription of DNA-directed RNA synthesis?

Initiation
- requires binding of protein general transcription factors and RNA polymerase to promoter sites up-stream of the coding region of a gene
- facilitated by transcription factors bound to enhancer sequences at sites far from gene
Elongation
- requires local unwinding of the DNA helix by RNA polymerase
- followed by synthesis of a 5’ to 3’ RNA transcript coded for by the DNA template read in the 3’ to 5’ direction
Termination
- requires a terminal signal sequence that results in release of RNA polymerase and newly synthesized transcript from DNA

Examples of post-transcriptional modification:

splicing of mRNA to eliminate non-coding introns and join exons
cleavage and trimming of pre-ribosomal RNAs
Trimming and modification in tRNA
Addition of a 3’ poly-A “tail” and a 5’-7-methyl guanosine “cap” to mRNA

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What are four examples of eukaryotic post-transcriptional modifications?

splicing of mRNA to eliminate non-coding introns and join exons
cleavage and trimming of pre-ribosomal RNAs
Trimming and modification of tRNA
addition of 3’ poly-A “tail” and 5’ methyl guanosine “cap” to mRNA

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A ______ is a segment of DNA that functions to generate RNA.

gene

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_______ catalyze phosphodiester bond formation between the alpha phosphate on the incoming NTP and the 3’-hydroxyl end of the RNA.

RNA polymerase

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Which of the following is false regarding prokaryotic RNA polymerase?

A. RNA polymerase (bacterial and eukaryotic) require a primer
B. RNA polymerase do not have either 3’ to 5’ exonuclease activity (proofreading) or 5’ to 3’ exonuclease activity
C. RNA polymerase consists of a core enzyme that contain alpha and beta subunits
D. RNA polymerase contains a holoenzyme which consists of the core plus a sigma
E. All of the above is true regarding prokaryotic RNA polymerase

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A. RNA polymerase (bacterial and eukaryotic) require a primer

RNA polymerase do NOT require a primer but do require promoters

note: sigma acts as a regulatory factor, guiding RNA polymerase to specific promoter sequences on the DNA template strand

***Holoenzyme = core enzyme (RNA pol) and sigma

Prokaryotic transcription requires a promoter. What some details regarding the promoter?

Where is the promotor located on DNA?

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the regulatory region of DNA generally located “upstream” of the coding sequence
contains DNA consensus (-35 and -10 elements) sequences that are recognized by RNA polymerase
Consensus sequence is a sequence that is mostly found in a given region when multiple sequences are aligned
Promoters are usually rich in TATA box

What are the steps in prokaryotic transcription?

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initiation begins when sigma binds to promoter region of DNA
Sigma opens the DNA helix; transcription begins
initiation is complete: sigma releases; mRNA synthesis continues
Elongation: transcription continues
Termination: hairpin forms “downstream”

What promoters do eukaryotes use that is different than prokaryotes?

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Eukaryotes = transcription factors

Prokaryotes = sigma

Which of the following eukaryotic RNA polymerases work solely on rRNA?

A. RNA pol I
B. RNA pol II
C. RNA pol III

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A. RNA pol I

RNA pol I = rRNA

RNA pol II = mRNA

RNA poll III = tRNA and rRNA

Which of the following eukaryotic RNA polymerases work on both tRNA and rRNA?

A. RNA pol I
B. RNA pol II
C. RNA pol III

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C. RNA pol III

RNA pol I = rRNA

RNA pol II = mRNA

RNA poll III = tRNA and rRNA

Which of the following eukaryotic RNA polymerases work solely on mRNA? A. RNA pol I B. RNA pol II C. RNA pol III

B. RNA pol II RNA pol I = rRNA RNA pol II = mRNA RNA poll III = tRNA and rRNA

(T/F) Eukaryotic rRNA, tRNA, and mRNA are processed but prokaryotic mRNA is generally not processed.

True ***Eukaryotic mRNA processing takes place in the NUCLEUS prior to mRNA translation in the cytoplasm. Processing of mRNA includes: - 5' capping - 3' polyadenylation - Intron splicing (in nucleus)

What is the purpose of 5' mRNA capping and 3' polyadenylation?

5' Capping w/ a modified guanosine is important for: - stability of mRNA - exit from nucleus 3' poly A tailing is addition of A's to the 3' end: - stability of mRNA - nuclear export mRNA - transcription termination * ** translation

Pre-mRNA is spliced to remove _______.

introns Takes place in spliceosome: Spliceosome is composed of: - small nuclear RNAs (snRNAs) - small nuclear ribonucleoproteins (snRNP) "snRPS" snRPS cut out introns

Describe alternative splicing:

Production of two (or more) distinct proteins from a single gene. Individual genes express multiple mRNAs "you can splice out exons to make different proteins if needed"

________ is an enzyme that synthesizes DNA from viral RNA. This enzyme synthesizes complementary DNA strand (cDNA). This process is extremely error-prone and mutation arise at a high rate, potentially leading to drug resistance.

Reverse transcriptase

What are the 3 stop codons?

UAA, UAG, UGA

There are _____ potential reading frames for a given mRNA sequence.

mRNA = 3 DNA = 6 (three for each strand)

________ refers to prokaryotic mRNAs that often have several coding regions (ORFs) on one mRNA.

Polycistronic

_______ occurs when a change in codon results in change in amino acid sequence. ``` A. Silent mutation B. Missense mutation C. Nonsense mutation D. Splice site mutation E. Frame-shift mutation ```

B. Missense mutation

______ occurs when a change in codon does NOT result in a change in amino acid sequence. ``` A. Silent mutation B. Missense mutation C. Nonsense mutation D. Splice site mutation E. Frame-shift mutation ```

A. Silent mutation

________ occurs when one or two nucleotide insertion or deletion throws the reading frame out of register resulting in shorter or longer protein with different protein sequence starting at the insertion/deletion site. ``` A. Silent mutation B. Missense mutation C. Nonsense mutation D. Splice site mutation E. Frame-shift mutation ```

E. Frame-shift mutation

______ occurs when a change in a codon results in change in amino acid to a stop codon. ``` A. Silent mutation B. Missense mutation C. Nonsense mutation D. Splice site mutation E. Frame-shift mutation ```

C. Nonsense mutation

_______ alter the way in which introns are removed producing aberrant proteins. ``` A. Silent mutation B. Missense mutation C. Nonsense mutation D. Splice site mutation E. Frame-shift mutation ```

D. Splice site mutation Silent mutation: change in codon does not result in change in AA sequence Missense mutation: change in codon results in change in AA sequence (sickle cell) Nonsense mutation: change in codon results in change in AA to STOP CODON (cystic fibrosis)

What is "Wobble"?

"Wobble" allows one anticodon to base pair with several different codons. If tRNA anticodon recognized only one codon, cells would have different tRNA for each codon (which they do not)

_______ is the most highly structured of all. A. mRNA B. rRNA C. tRNA

C. tRNA

_________ "reads" the codons. A. mRNA B. rRNA C. tRNA

C. tRNA note: anticodon of tRNA base pairs with the codon in mRNA in an antiparallel and complementary fashion

______ are a large molecular motor.

Ribosomes

_________ are enzymes that couple ("charge") amino acids to their cognate tRNA. Correctly pairing the amino acid with the tRNA is important for the proper transmission of genetic information.

Aminoacyl-tRNA synthetases note: they basically add amino acids to the tRNA by utilizing ATP

________ is a purine rich sequence upstream of AUG start codon that interacts with rRNA to register the reading frame such that INITIATION begins at AUG.

Shine-Dalgarno sequence

Describe each of the following steps in protein synthesis: - Initiation - Elongation - Termination

Initiation: - small subunit attaches - tRNA-AA recognizes - initiation factor is lost when large subunit attaches A site = Aminoacyl site P site = Peptidyl site E site = Exit site Elongation: 1. binding of incoming aminoacyl-tRNA 2. Peptide bond formation 3. Translocation Termination: - stop codon occupies the A site - Release factors (grenade) hydrolyze the terminal peptidyl bond - Release of polypeptide - Dissociation of the ribosome

Describe polysomes:

Many ribosomes will utilize the 1/2 life of mRNA

Which of the following is contains a modified guanosine cap? A. 5' end B. 3' end

A. 5' end ***5' cap ***3' Poly A tail

Module 2: RNA & Protein Flashcards

(47 cards)