molecular bio 6

Question 1

N C terminus, 3 and 5 side

Question 2

Translation regulation

Answer 2

Not transported, degraded, miRNA

Question 3

If you do MS, do you know all the protein sequences? How do you know which proteins are which using MS? Doesn’t RNAseq give you more info than proteomics?

Question 4

To do RNAseq, what do you do to the RNA first?

Answer 4

mRNA > cDNA > PCR

Question 5

Do you get absolute or ratiometric data from MS?

Answer 5

ratio

Question 6

How much of the variance of protein is explained by mRNA concentration in humans?

Answer 6

27%

Question 7

Why can coding sequence help predict protein concentration?

Answer 7

Some transcripts require additional initiation factors

Question 8

If your antibodies are differently good, then it’ll

Question 9

other approaches

Answer 9

capillary immunoblotting
ELISA - contain antibodies and add lisates to see if it lights up

Question 10

mass spec for proteins

Answer 10

1. digest it, not sonification, with peptidases
2. ionize - some have r group that can be ionized

Question 11

what does mass spec measure?

Answer 11

mass to charge ratio

Question 12

Before MS, why would you run an SDS-page?

Answer 12

Isolate a particular set of proteins

Question 13

Proteases

Answer 13

all have different places where they cleave

Question 14

How does imaging MS work??

Answer 14

I think just you do MS on different tissues and color them in post…?

Answer 15

I think you can use MS to get a list of proteins in your sample :o

Question 16

quantitative ms

Answer 16

add a protein with known amount “spike control”?

Question 17

Some posttranslational modifications

Answer 17

have too weak of a m/z change that you won’t know