molecular biology Flashcards

(37 cards)

1
Q

what are two kinds of nitrogenous bases

A

purines (A,G)
Pyrimidines (C, T, U)

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2
Q

what was the experiment where they used mice to conduct an experiment about prokaryotic bacterium transformation

A

Frederick Griffiths Experiment

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3
Q

what was the hypothesis of the Frederick Griffiths experiment

A

macromeolcule from S bacteria, which functions as the genetic material, will be able to convert type R bacteria into type S

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4
Q

what was the bacteriophage infection experiment called

A

The Hershey-Chase Experiments

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5
Q

what did they do in the Hershey-Chase Experiments

A

they fed T2 infected bacteria with radioactive compounds (phosphorus and sulphur) and added them into viral DNA and proteins, respectively

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6
Q

what were the results of the Hershey-Chase Experiments

A

the virus DNA, not the proteins entered the host bacterial cells

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7
Q

what do nucleotides consist of

A

a nitrogenous base
a pentose (5 carbon sugar)
a phosphate group

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8
Q

what does a nucleotide structure consist of

A

phosphate, thymine, deoxyribose

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9
Q

in RNA, what replaces thymine

A

Uracil

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10
Q

what bonds the sugar-phosphate backbone

A

phosphodiester bonds

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11
Q

what is the DNA sequence prime

A

read from the 5’ end to the 3’ end

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12
Q

who discovered that the structure of DNA was a double-stranded helix

A

James Watson and Francis crick

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13
Q

DNA molecules consist of two strands running in what direction

A

opposite

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14
Q

how do nitrogenous bases connect in the middle

A

through H-bonds

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15
Q

t or f RNA is double stranded

A

false it’s single stranded

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16
Q

what does mRNA do

A

a transcript of the gene that carries information for the synthesis of proteins

17
Q

what does tRNA do

A

translater between nucleic acid and protein by picking up specific amino acids and recognizing the appropriate codons in mRNA

18
Q

what does rRNA do

A

most abundant type of RNA
together with proteins forms the structure of ribosomes

19
Q

how is genetic information coded in the DNA molecule

A

information must lie on the linear sequence of nitrogenous bases, (arrangement of bases)

20
Q

How is the information passed on from one cell to the next

A

because of the complementary base pairing of nitrogenous bases
that one strand serves as a template to form a new strand (DNA replication)

21
Q

how does the genetic material function

A

the DNA passes on its information (transcription) used in cell function

22
Q

what are the three possible mechanisms for DNA replication

A

semiconservative
conservative
dispersive

23
Q

what happens during the semiconservative mechanism

A

DNA replication produces DNA molecules with 1 parent strand and 1 newly made strand

24
Q

what happens during the conservative mechanism

A

dna réplication produces 1 double helix with both parental stands, and the other with 2 daughter strands

25
what happens during dispersive mechanism
segments of new DNA are with the parental DNA
26
what experiment showed that DNA replication is semi-conservative
meselson and stahl experiment
27
what is the untwisting of the DNA molecule
breaking H-bonds between N-bases by certain enzymes
28
what enzyme helps in linking of free nucleotides to form a new strand
DNA polymerase
29
what end are nucleotides added at
3'
30
What links DNA pieces
DNA ligase
31
what is a gene expression
flows of genetic information from the genotype to the phenotype
32
what is transcription
information encoded in DNA is made into complementary RNA. Said to produce mRNA
33
what is translation
process of synthesizing a specific polypeptide by using information encoded in the mRNA on a ribosome
34
in transcription what does the promoter do
site where RNA polymerase binds to begin transcription
35
in transcription: what does regulatory sequences do
site binding to regulatory proteins control whether a gene is turned on or off
36
in transcription what is the transcribed region
a region of DNA that is transcribed into an RNA molecule
37
in transcription what is the terminator
sequence that causes the RNA strand to be released from the transcription complex