Molecular Biology

Question 1

What DNA binding motif has the following characteristics?

• is the simplest DNA-binding motif
• made up of two alpha helicies connected by short chain of amino acids that make the turn at a fixed angle
• longer helium portion= recognition module and DNA binding module
• their proteins bind as dimers

Answer 1

Helix-turn-helix

Question 2

What DNA binding motif has the following characteristics?

-DNA binding motif includes Zn atom

Answer 2

Zinc finger domain

Question 3

What DNA binding motif contains the following characteristics?

• two alpha helical DNA binding domain
• dimerizes
• interactions between hydrophobic amino acid side chains at every 7 amino acids down one side of alpha helix
• grabs DNA like a clothespin

Answer 3

Leucine Zipper motif

Question 4

What DNA binding motif contains the following characteristics?

• Consists of a short alpha chain connected by a loop to a second longer alpha chain
• can occur as homodimers or heterodimers
• three domains: DNA binding domain, dimerization domain, activation domain

Answer 4

Helix-loop-helix

Question 5

What works to get all of the components together to control gene transcription?

Answer 5

Mediators

Question 6

What is the primary regulator of the stability of the RNA?

Answer 6

Poly-A-Tail

Question 7

All of the following are examples of what?

• Proteins folding into their 3-D conformations
• molecular chaperones help proteins fold appropriately
• bind co factors

Answer 7

Post translational modifications

Question 8

What acts to destroy proteins that are ubiiqutinated?

Answer 8

Proteasomes

Question 9

T/F: one E1 ubiquitin activating enzyme and 1 proteasome but 30 E2 ubiquitin conjugating enzymes and hundreds of E3 accessory proteins

Answer 9

True

Question 10

What control of gene expression is defined by what genes get expressed (or not) from mom and dad?

Answer 10

Methylation and genomic imprinting

Question 11

What control of gene expression occurs for a woman to have the same number of genes expressed as men?

Answer 11

X chromosome inactivation

Question 12

What checkpoint is between G1 and S where the cell enters the cycle and proceeds to S phase if the environment is favorable

Answer 12

Start checkpoint

Question 13

What checkpoint is the point where if all the DNA is replicated and the environment is favorable enter mitosis?

Answer 13

G2/M checkpoint