molecular genetics

Question 1

nucleic acid

Question 2

nucelotides (draw)

Question 3

building nucleaic acids

Question 4

difference sbetween dna and rna

Question 5

dna replication:

Answer 5

semiconservative

Question 6

requirments for dna replication

Question 7

steps of dna replication

Answer 7

1. helicase unzips souble helix

Question 8

the enzymes involved in dna replication

Answer 8

helicase, gyrase ssb
sbna primase
dna polymerase 3
dna polymerase 2
ligase

Question 9

the centra dogma

Question 10

transcription: types of rna and where theyre found

Question 11

requirements for transcription

Question 12

steps of transcription

Question 13

enzymes involved in transcriotuon

Answer 13

• rna polymerase
  transcription factors
  splicesomes

Question 14

where does replication, transcripton, and translation happen

Answer 14

rep: in nucleus
transcription: in nucleus
translation: ribosomes

Question 15

translation

Question 16

requirements for translation

Question 17

enzymes of translations

Answer 17

• small and large ribosomal subunit
• aminoacylt-t rna synthetase
• relaese factors

Question 18

steps of translation

Question 19

ribosomes

Question 20

function of trna

Question 21

start codon

Question 22

immature mrna and post transcriptional eiting

Question 23

types of mutations

Answer 23

point and chromosomal

Question 24

silent mutations

Answer 24

missesne mutations

Question 25

nonsense mutations

Answer 25

d

Question 26

deletion mutations

Question 27

insertion mutations

Answer 27

t

Question 28

translotcation mutatins

Question 29

inversion mutations

Question 30

gene regulation

Question 31

lac operon and how it works

Question 32

lac operon ON

Answer 32

a

Question 33

lac operon OFF

Question 34

lacz lacy laca

Question 35

trp operon

Question 36

trp ON

Question 37

trp OFF

Question 38

eukaryotic gene expression transcription level a) transcriptional

Question 39

eukaryotic gene expression transcription level b) post transcriptional

Question 40

restriction endonucleases

Question 41

restriction enzymes

Question 42

sticky ends

Question 43

blunt ends

Question 44

RFLP

Question 45

GEL electrophoreisis (steps and uses)

Question 46

polymerase chain reaction

Question 47

taq polymerase

Question 47

transformation

Answer 47

(heatshock and electroporation)