Monoclonal antibodies Flashcards
What kind of things can antibody engineering and glycosylation achieve?
reduced immunogenicity.
improved potency for target.
Increased complement activation and Fc receptor binding.
Increased stability and half life.
Ending for chimeric antibodies and what method used.
ximab and uses hybridoma
What two have the most competent and ADCC out of the antibody subclasses?
IgG1 and IgG3
What is the best inducer of complement out of the sublasses?
IgM
how do you humanise mAb?
Take the bits from the CDR3
How do you make human like Ab without immunising people with phage display?
Phage has coat protein with PIII that can be fused with human Ab VH and VL gene, then binds and replicates and enriches in bacteria expressing that antigen.
Problem with phage display dreived Ab?
They may not have high affinity for self molecules and haven’t gone through SHM.
An alternative way to create human-like Ab?
WIth transgenic mice expressing human Ab genes and then immunise them- affinity maturation.
mouse ending?
chimeric
humanised
omab
ximab
zumab
umab
some issues with higher affinity for mAb?
won’t penetrate to target tissue
cross-reactivity and greater side effects
dose reduction limited by antigenic sink
What are Gentuzumab and Irbritumumab used for?
Have anti cancer drugs (or radioisotpe) conjugated
What does Trastuzumab do?
Blocks HER2 in cancer
What does bevacizumab do?
targets VEGF for cancer vasculature inhibition.
What is an anti-CTLA-4 mAb?
ipilimumab
What doesn’t anti CD20 deplete?
plasma cells.
