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Bacte LAB > MORPHOLOGY And SMEAR PREPARATION > Flashcards

MORPHOLOGY And SMEAR PREPARATION Flashcards

(56 cards)

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1
Q

Bacterial Morphological Types

A

A. Cocci
B. Bacilli
C. Spiral

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2
Q

Bacterial Structures

A

A. Spore
B. Capsules
C. Granule
D. Flagella

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3
Q

Laboratory Preparations

A

A. Wet Mount
B. Hanging Drop
C. Fixed Stained Smear

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4
Q

causative agent for pneumonia

A

Streptococcus pneumoniae

COCCI

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5
Q

-> causative agent for skin infections

A

Staphylococcus aureus

COCCI

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6
Q

Types of COCCI

A

Monococcus
Diplococcus
Staphylococcus
Tetracoccus
Sarcina
Streptococcus

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7
Q

COCCI
•_______ in shape
In GREEK. it means “______”

A

SPHERICAL or ROUND

BERRY

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8
Q

BACILLI
• shape..

In LATIN, it means “_____’

A

Straight, Cigarette-shaped, Sausage, Elongated

LITTLE STICKS

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9
Q

Types of BACILLI

A

Bacillus
Coccobacillus
Palisades
Diplobacillus
Spore-former
Streptobacillus

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10
Q

-› also termed as “Chinese letter”

A

Palisades

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11
Q

causative agent for botulism or food poisoning

A

Bacillus

Clostridium botulinum

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12
Q

-› found in Yakult

A

Coccobacillus

Lactobacillus casei

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13
Q

-› causative agent for diphtheria

A

Palisades

Corynebacterium diphtheria

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14
Q

SPIRAL
• shape

A

CURVED RODS, HELICOIDAL, CORK-SCREW

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15
Q

Curved shapes of bacteria

A

Vibrious
Spirilla
Spirochetes

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16
Q

-> causative agent for cholera

A

Comma

Vibrio cholerae

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17
Q

-› causative agent for syphilis

A

Spirochete

Treponema pallidum

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18
Q

• SPHERICAL or ROUND in shape
In GREEK. it means “BERRY”

A

COCCI

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19
Q

• Straight, Cigarette-shaped, Sausage, Elongated In LATIN, it means “LITTLE STICKS’

A

BACILLI

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20
Q

• CURVED RODS, HELICOIDAL, CORK-SCREW

A

SPIRAL

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21
Q

• Actual body parts of the bacteria
• Makes bacteria unique
• Narrows down the bacteria need to be identified
• Cannot be demonstrated using a routine staining method

A

BACTERIAL STRUCTURES

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22
Q

SPORE
• Example:

A

Bacillus anthracis

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23
Q
  • resting stage of bacteria;

highly resistant to extreme conditions

24
Q

• The structure that forms inside bacteria

Common or routine stains cannot penetrate it

25
Endospore The ability to resist stain may be attributed to its chemical composition:
Calcium dipicolinic acid peptidoglycan complex
26
• substance making spore highly resistant to physical and chemical antimicrobial agents
Calcium, dipicolinic acid, peptidoglycan complex
27
> not synthesized by vegetative cell only synthesized during spore formation
• dipicolinic acid
28
• Only one is formed from a single bacterium • Not a process for multiplication but formed as a defense mechanism
Spore
29
Spore found at the tip of bacterium -*
terminal
30
Spore found near the tip of bacterium -›
subterminal
31
• Spore at the middle of bacterium -›
central
32
CAPSULES • Example:
Streptococcus pneumoniae
33
• GLYCOCALYX or SUGAR COAT
Capsules
34
-> A general term used for substances surrounding the cell - a gelatinous substance that the cell secretes externally
GLYCOCALYX or SUGAR COAT
35
- The formation is dependent on where the bacteria grew - help in determining treatment for a disease
GLYCOCALYX or SUGAR COAT
36
Glycocalyx/ sugar coat -> made up of_______ or _____ or _____
polysaccharide or polypeptide or both
37
2 types of glycocalyx
Capsule Slime layer
38
› firmly attached to the cell wall of bacteria; responsible for protecting pathogenic bacteria from phagocytosis
capsule
39
-› used for adhesion
slime layer
40
The background will be____; the ***capsule*** appears as a____ zone
stained clear
41
GRANULE Example:
Corynebacterium diphtheria
42
-> store carbohydrates (stain brown with iodine) - "iogen" granules
Granule
43
• Referred to as inclusion bodies • Demonstrated by staining and other chemical means
Granules
44
Granules are formed when
abundant nutrients from the environment are being deposited on reserves in the cell -> granules/inclusion podies
45
"Little whips" • Thin processes usually longer than the bacteria they originate from
FLAGELLA
46
› fibers come together to make up flagella; It undergo expansions and contractions; contractile (same with the concept of actin and myosin)
Flagellim
47
Types of flagella arrangement
Monotrichous Lophotrichous Amphitrichous Peritrichous
48
LABORATORY PREPARATION AND EXAMINATION FOR MOTILITY AND MORPHOLOGY A. WET MOUNT • Bacteria examined must be___
alive
49
Wet mount Process: - Bacterial sample (from broth or agar) is placed on a drop on____ or ____ on the slide -› Cover with a coverslip. Examine under microscope
NSS or deionized water
50
HANGING DROP PREPARATION • Bacteria examined must be___ • Make use of a glass slide with a____ on it (can be made from paraffin wax) or use commercially prepared slides
alive ring
51
Hanging drop preparation • Process: - Coverslip is added with a drop of____ -> The glass slide is allowed to stick with the coverslip - Drop that was placed on the coverslip is "hanging" if glass slide is added and placed under the microscope
bacteria
52
FIXED STAINED SMEAR • There is a need to____ the bacteria • The____ procedure will render bacteria immobile
kill staining
53
• Bacteria examined must be alive • Make use of a glass slide with a ring on it (can be made from paraffin wax) or use commercially prepared slides
HANGING DROP PREPARATION
54
• There is a need to kill the bacteria • The staining procedure will render bacteria immobile
FIXED STAINED SMEAR
55
-> is a type of preparation that bacteriologists use to start the determination of bacterial identity
• Bacterial Smear
56
• Bacterial Smear - bacteria is heated to be fixed on the slide; heat fixing must be done___ ->_____ coagulate which will make visualization on the slide better -> cells will become distorted to some extent
rapidly cytoplasmic proteins

Bacte LAB (38 decks)

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