MTAP 1 10/15/2022

Question 1

gel test technology was developed by

Answer 1

Dr. Yves Lapierre in 1985

Question 2

gel test technology: gel type

Answer 2

dextran polyacrylamide gel

Question 3

gel test technology

microtube upper portion is

Answer 3

reaction of chamber

Question 4

gel test technology

microtube lower portion is

Answer 4

column

Question 5

gel test technology principle

Answer 5

size exclusion chromatography

Question 6

type of gel tests

Answer 6

1. specific
2. plain / neutral
3. low ionic antiglobulin

Question 7

type of gel test specific for target antibody/antigen

Answer 7

specific

Question 8

Type of gel test
- The gel is not yet dispensed with reagent
- Add the reagent along with the sample in the microtube

Answer 8

Plain / Neutral

Question 9

gel test that do Reverse/Backward/Indirect/Serum Typing

Answer 9

plain / neutral

Question 10

test gel specific for sensitization detection

Answer 10

low ionic antiglobulin

Question 11

low ionic antiglobulin reagent added into the matrix

Answer 11

AHG reagent

Question 12

Agglutination reaction in gel medium can be reviewed for _ DAYS (clump is stable)

Answer 12

3

Question 13

gel test technology sample volume

Answer 13

<1ml

Question 14

gel test:

The gel matrix itself traps the unbound _ that may interfere with the reaction

Answer 14

antibodies

Question 15

grading of RBC most cells at the center portion in gel test

Answer 15

2+

Question 16

grading of RBC near the bottom portion in gel test

Answer 16

weak (+/-)

Question 17

antihuman globulin test is also known as

Answer 17

Coombs Test

Question 18

end product of lattice formation

Answer 18

agglutination

Question 19

best agglutinating antibody

Answer 19

IgM

Question 20

Incomplete IgG antibodies are only capable of _ the red cell antigens.

Answer 20

sensitizing

Question 21

principle of antihuman globulin test

Answer 21

detection of cell-bound immunoglobulin / incomplete antibodies (IgG)

Question 22

cold reacting antibody is best reactive at what temperature?

Answer 22

1-6deg C

Question 23

saline-reactive immunoglobulin

Answer 23

IgM

Question 24

albumin / ahg reactive immunoglobulin

Answer 24

IgG

Question 25

IgM ab example IgG ab example

Answer 25

ABO Rh

Question 26

IgG Most to least potent:

Answer 26

3,1,2

Question 27

type of AHG reagents

Answer 27

polyspecific AHG reagent monospecific AHG reagent

Question 28

polyspecific ahg reagent is consist of

Answer 28

- pool of rabbit anti-human IgG - mouse monoclonal anti-C3d and anti-C3b

Question 29

Polyspecific AHG Reagents are also refereed to

Answer 29

Broad Spectrum Coombs Reagent

Question 30

Add _ to distinguish AHG from other different reagent

Answer 30

green dye

Question 31

Method used for Monospecific reagent

Answer 31

Hybridoma Technique/Kohler and Milstein Technique

Question 32

cells combined in forming hybridoma cells

Answer 32

mouse plasma cells and myeloma cells

Question 33

stages of ab-ag interaction

Answer 33

- sensitization - agglutination

Question 34

DAT detects _ sensitization

Answer 34

in vivo

Question 35

Immediate HTR is severe and fatal and usually caused by

Answer 35

ABO incompatibility

Question 36

DAT collection tube

Answer 36

EDTA and citrate

Question 37

chelation of calcium holds _ subunits (_) together

Answer 37

C1; q,r,s

Question 38

largest complement protein

Answer 38

c1

Question 39

wharton’s jelly can cause

Answer 39

rouleaux formation

Question 40

presence of unbound antibodies can _ the AHG reagent resulting to

Answer 40

neutralize; false negative

Question 41

Delay in the addition of reagent = antibodies may undergo

Answer 41

ELUTION PROCESS

Question 42

conventional method of DAT

Answer 42

- washing - ahg addition - centrifugation - dislodge - observe

Question 43

minimum ration of serum to cells in AHG Test

Answer 43

40:1

Question 44

in AHG Test, drops of serum and cells

Answer 44

2 drops serum and 1 drop of 5% v/v cell suspension

Question 45

too high temperature in AHG Test can cause

Answer 45

protein denaturation

Question 46

too low temperature in AHG Test can cause

Answer 46

IgG inactivation

Question 47

in AHG Test incubation time in saline suspension

Answer 47

30-120 minutes

Question 48

in AHG Test incubation time in LISS suspension

Answer 48

10-15 minutes

Question 49

in AHG Test saline for washing; should be fresh and buffered to a pH of _

Answer 49

7.2-7.4

Question 50

in AHG test centrifugation time

Answer 50

1000 rcf for 15-20 seconds