mtap 2 rationale Flashcards by Christianne Espiña

1st Ab detected after primary infection

IgM

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1st Ab detected after exposure to Ag

IgM

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Ab best at agglutination and complement fixation

IgM

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Enhance of phagocytosis by coating of foreign particles with serum proteins

opsonization

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a cell that secretes Ab

B cell

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involves interaction between soluble Ag and soluble Ab

precipitation

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normal flora of the mouth

Viridans streptococci

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increases in parasitic infections and allergies

eosinophils

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defect in oxidative or respiratory burst

chronic granulomatous disease

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true or false:
null cells are lymphocytes

true

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B cells can be distinguished from other lymphocytes by the presence of __ in their surface

surface immunoglobulins

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variable region of Ig are found in the:

amino terminal end, Fab portion

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Ig that can activate classical complement cascade

IgM and IgG

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Ig in the surface of B cells

IgM and IgD

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Ab predominant in secretions

IgA2

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Ig that can cross placenta

IgG

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optimal ratio when most Ab is precipitated by least amount of Ag

zone of equivalence

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Ig in greatest amount and rises later in infection

IgG

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IgM molecule is a___

pentamer

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In indirect antiglobulin test, how many times must the mixture be washed with saline?

3 times

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what causes false negative in agglutination reactions?

inadequate washing of cells in AHG test

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Ig assoc. with type 1 hypersensitivity reaction

IgE

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amount of Ag binding sites of IgM

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a cell notable when there are helminth infections

eosinophils

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serum factors in the blood that are formed in response to foreign substances

antibodies

important effector cells in allergic reaction

basophils and eosinophils

WBC capable of further differentiation

monocyte

stages of agglutination

1. sensitization 2. lattice formation

indirect phagocytosis occurs through:

opsonization

monocyte-macrophages found in the kidneys are known as:

mesangial cells

T cells account to ___ of total lymphocytes

60-80%

Ab are characteristically:

protein | only!!

Ig with CH4

IgM and IgE

Ig with joining chain

IgM and IgA

most predominant Ab in serum

IgG

Post zonal reaction is caused by:

antigen excess

binding strength of Ab for Ag

Avidity

Why do we wash in indirect antiglobulin test?

remove unbound antibodies

what causes false-positive in agglutination?

contaminated glassware, slides, or reagents

why do we use antiglobulin reagent in direct antiglobulin test?

detect in vivo sensitization

Prozone occurs when___

antibody are in excess of antigen

routine test for blood products

HIV-1 (malaria test is also used here in PH)

how are Rh Ab usually characterized?

Immune IgG

Ideal reaction for Rh Ab

incubation at 37C, enhancement media, and enzymes

how are ABO Ab usually characterized?

naturally occuring, cold reactive, IgM

can frozen RBCs be thawed and washed at 9am be transfused at 11am the next day?

No, units are expired (only viable for 24hrs after thawing)

A unit of pRBCs was split using open system. 1/2 units was used. What may be done on second half?

must be issued within 24hrs

components stored at RT, 20-24C

Platelets and granulocytes

storage temp and expiration date for granulocytes

20-24C, expiration in 24hrs

hepa B markers routinely tested for donor blood

HBsAg and anti-HBc

purpose of adenine in blood bags

increases ADP levels

storage temp of RBCs

1-6C

most blood group Ab are of what Ig classes?

IgG and IgM

Ab generally detected at antiglobulin phase (warm reactive) of testing:

anti-Jka

binding strength of Abs for multivalent Ag

avidity

most dangerous and unexpected Ab in blood bank are those that react at:

37C (body temp)

condition that would contraindicate autologous presurgical donation

has bacteremia, cardiovascular problem, and pulmonary problem

blood group system associated with IgM

Lewis

technique that removes antibody bound to sensitized red cells

elution

optimum incubation temp for indirect antiglobulin test

37C

immunodominant sugar responsible for blood group A specificity

N-acetyl-D-galactosamine

result from storage lesion

increase in plasma K

1 unit donor platelet-derived from whole blood (RDP) should yield ___ platelets

5.5 x 10^10

1 unit donor platelet-derived from plateletpheresis (SDP) should yield ___ platelets

3.0 x 10^11

storage temp of pRBC

1-6C

storage temp of fresh frozen plasma

-18 or colder

designated by the terms Rh positive and Rh negative

presence or absence of D antigen

Ab enhanced by acidifying patient serum

Anti-M

can autologous donation of RBCs frozen in 1989 be used in 1994?

Yes, it is good for 10years

Hematocrit acceptable for pRBC in closed system?

less than 80%

component of choice for treating von Willebrand disease

cryoprecipitate

component of choice to prevent graft-vs-host-disease

irradiated component

storage life of blood anticoagulated using CP2D

21 days

what will happen if you use 10% red cell suspension for compatibility testing?

false negative result due to antigen excess

washed RBCs are product of choice for a patient with:

Anti-IgA antibodies

blood group system that causes the **most serious hemolytic reactions**

ABO

posttransfusion **anaphylactic** reactions occur most often in patients with:

IgA deficiency

purpose of low-dose irradiation of blood components

prevent graft-vs-host disease

additive solution contains:

- saline - adenine - dextrose/glucose - mannitol

present in rejuvenating solution

inosine

pRBCs obtained thru open system using CPDA1 has a shelf life of:

24hrs

required minimum weight of donor

110lbs | 50kg

frozen RBCs processed in open system can be stored for how many days/hours

24hrs

polyethylene glycol enhances ag-ab reactions by

concentrating ab by removal of water

shelf life of a unit of blood collected in CPDA1

35 days

how many units of AHF activity does a unit of **cryoprecipitate** has?

80IU

once thawed, FFP must be transfused within:

24hrs

what does fresh frozen plasma does not provide?

platelets

last unit of autologous blood should be collected no later than ___ hours before surgery

72hrs

temperature range for maintaining RBCs and whole blood during shipping is

1-10C

mtap 2 rationale Flashcards

(90 cards)