multiple choice

Question 1

Which of the following best describes the function of the 5’-UTR in the SARS-CoV-2 RNA genome?
A. It encodes structural proteins necessary for viral assembly.
B. It facilitates the cap-independent translation of viral proteins.
C. It regulates translation initiation through stem-loop structures.
D. It promotes viral RNA degradation by host enzymes.
E. It inhibits viral replication through RNA interference.

Answer 1

C. It regulates translation initiation through stem-loop structures.

Question 2

Why did the researchers use yeast tRNA as a negative control in the RNA pulldown assay?
A. To confirm that RBM24 binds to viral RNA specifically.
B. To verify that RBM24 binds to all RNA molecules equally.
C. To measure non-specific RNA degradation.
D. To act as a positive control for ribosome assembly.
E. To ensure that RBM24 is not a ribonuclease.

Answer 2

A. To confirm that RBM24 binds to viral RNA specifically.

Question 3

Which of the following RNA motifs was identified as the primary binding site for RBM24 in the SARS-CoV-2 5’-UTR?
A. GCGCG
B. GUGUG
C. CACAC
D. AUUUA
E. CUGCU

Answer 3

B. GUGUG

Question 4

What was the purpose of using pHA-RBM24ΔRNP1/2 in Figure 2?
A. To assess the effect of complete RBM24 knockout.
B. To determine the role of individual RNP domains in RBM24 binding.
C. To test the impact of deleting both RNP domains on viral RNA binding and inhibition.
D. To compare the antiviral effects of RBM24 and its mutants.
E. To confirm RBM24’s localisation in the nucleus.

Answer 4

C. To test the impact of deleting both RNP domains on viral RNA binding and inhibition.

Question 5

According to the study, which of the following viruses also contain G(U/C/A)GUG motifs similar to those targeted by RBM24?
A. RSV, CMV, HSV, Influenza
B. Dengue, Rabies, Parvovirus, HIV-1
C. Zika, MERS, Ebola, HCV
D. Adenovirus, Measles, Poxvirus, HBV
E. Poliovirus, Rhinovirus, Hepatitis A, HSV

Answer 5

C. Zika, MERS, Ebola, HCV ✅