mycobacteria

Question 1

Mycobacteria TB

Answer 1

• starts in lungs, form lesions, spreads to O2 rich areas like kidney, long bones, lymph on macrophages
• spread by coughing up sputum
• immunocompromised people more at risk - cant rid infection
• immunocompetent people can get rid infected macrophages but latent infection remains in granulomas
• may become active again and appear as scrofula in neck or in GI, long bones, CNS
• kids may get TB meningitis
• very resistant; treat with 4 drugs
• mycolic acid in cell wall

Question 2

Mycobacteria Leprae

Answer 2

• only 5% people susceptible
• spread by nasal secretions and skin lesions
• cant grow in vitro. prefer 30C
• replicates in histocytes, schwaan cells, epithelial
• cause nerve damage
• wide range of symptoms. some have large immune response (+PPD) and others poor immune response (-PPD)

Question 3

acid fast stain procedure

Answer 3

1. carbofushion (bright pink)
2. boil
3. cool
4. decolorize - with acid/alc
5. counterstain and rinse