MYCOBACTERIUM

Question 1

-lowenstein Jensen Media
-cyanogen bromide
- yellow +
-no color -
-M. tuberculosis and M. intracellulare

Answer 1

Niacin Test

Question 2

sodium nitrate broth
N-n-dimethyl-l-naphthylamine sulfanilic acid
To confirm unreduced nitrate, add zinc dust/zinc powder + no color change: - red
+ red
Blue using strip +
M. tuberculosis and M. intracellulare

Answer 2

Nitrate reduction

Question 3

Lowenstein Jensen Media
Ferric ammonium citrate
Rusty brown color +
No color change -

Answer 3

Iron Uptake Test

Question 4

Detection of rapid growers
Phenolphthalein media
2N sodium carbonate
Pink +
No color change -
M. fortuitum and M. intracellulare

Answer 4

Arylsulfatase Test

Question 5

Pyrazinamidase agar
1% ferrous ammonium sulfate
Red +
No color change -

Answer 5

Pyrazinamidase Test

Question 6

Tween 80 media
Heating at 68 degrees
Cooling at 20 mins
Add 30% h2o2
Bubbling +
No bubbling -
More than 45 mm +
Less than 45-
M. fortuitum & M . gordonae and M. tuberculosis

Answer 6

Heat stable catalase test

Question 7

For identification of M. kansasii
+ in 3 days
M. tb in 10-20 days
Pink +
No color change -
Neutral red: indicator
M. kansasii and M. Intracellulare

Answer 7

Tween 80 hydrolysis test

Question 8

M. avium identification (TB-AIDS)
smooth fine black precipitate (smoke like action) +
Gray clumps no smoke like action -
M. avium and M. TB

Answer 8

Tellurite Reduction Test

Question 9

Identification of rapid growers and M. trivale
growth +
no growth-
5% NaCl broth
M. fortuitum and M. gordonae

Answer 9

Salt Tolerance test 5% NaCl

Question 10

Thiopene 2 carboxylic hydrazine
diff. M. TB and M. bovis
growth inhibition +
growth -

Answer 10

T2H/ TCH susceptibility

Question 11

M. fortuitum-chelonae complex identification

Answer 11

MAC w/o crystal violet

Question 12

TB in cattles
Intestinal TB in man

Answer 12

Mycobacterium bovis

Question 13

Culturally water droplets colonies

Answer 13

Mycobacterium bovis

Question 14

Nitrate and niacin negative to diff from M. TB

Answer 14

Mycobacterium bovis

Question 15

Hansen’s Bacillus
cigar packet arrangement
Non culturally on artificial media
Can be in mice foot pad or foot pads of armadillo

Answer 15

Mycobacterium leprae

Question 16

Cause leprosy
Prolong skin contact with infected person
Inhalataion
Leonine face

Answer 16

Mycobacterium leprae

Question 17

Mycobacterium leprae:
Less severe
Few organisms

Answer 17

Tuberculoid type/ paucibacillary

Question 18

Mycobacterium leprae:
can be malignant
Increased number of organism

Answer 18

Lepromatous/ multibacillary

Question 19

AFB within macrophage
Fire Faraco: hematoxylin stain as counter stain

Answer 19

Lepra cells

Question 20

2 NTM to fuse TB in those with AIDA

Answer 20

Mycobacterium kansasii

Question 21

Aka Cross Barred Bacillus

Answer 21

Mycobacterium kansasii

Question 22

Fish tank granuloma or Swimming pool granuloma skin dse

Answer 22

Mycobacterium marinum

Question 23

Mycobacterium asiaticum, M. simiae , M. intermedium & M. novocastrense

Answer 23

Photochromogens

Question 24

Pulmonary dse, predominantly in middle aged men
Cervical adenitis
Bursitis

Answer 24

Mycobacterium szulgai

Question 25

Cervical adenitis in children Bacteremia Pulmonary dse Skin infection

Answer 25

Mycobacterium scrofulaceum

Question 26

BSL 1 Tap water bacillus

Answer 26

M. gordonae/ M. aquae (old name)

Question 27

Primarily pulmonary infections in adults Birds nest appearing colonies on Corn Meal Agar (media for fungi)

Answer 27

M. xenopi

Question 28

M. flavescens , M. thermoresistible M. interjectum - Unknown Potentially pathogenic Chronic lymphadenitis, pulmonary disease Others: M. heckeshornense, M. tusciae, M. kubicae, M. cookie, M. hiberniae

Answer 28

Scotochromogens

Question 29

Lady Windermere Syndrome

Answer 29

M. avium

Question 30

Battey Bacillus

Answer 30

M. intracellulare

Question 31

Buruli ulcer Biochemically inert

Answer 31

M. gastri and M. ulcerans

Question 32

Dessiminated dse in patients with aids

Answer 32

M. malmoense and M. genovense

Question 33

M. haemophilum , Others: M. heidelbergense; M. shimoidei

Answer 33

NON PHOTOCHROMOGENS

Question 34

Breadcrumbs growth on LJM

Answer 34

M. phlei