Mycobacterium Flashcards

0
Q

Negative for T 80 HOH

A

M. avium

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1
Q

Slow growers except?

A

M. fortuitum

M. chelonei

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2
Q

AFB (mycolic acid)

A

true

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3
Q

Middlebrook 7H10-7H11-AST

A

Agar base

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5
Q

LJ Medium

A

Egg base

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6
Q

1+

A

10-99 AFB per 100 fields

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7
Q

Inhibitor of Egg base medium

A

Malachite Green

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10
Q

M. avium complex

A

Lady Windermere syndrome

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11
Q

Intestinal TB

A

MB bovis

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12
Q

Duboi’s oleic acid albumin medium

A

Agar base

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14
Q

Negative for HSCT

A

M. TB

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16
Q

BACTEC 12 B

A

Liquid base

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17
Q

Source of intestinal TB

A

cattle

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19
Q

3+

A

> 10 per field in at least 20 fields

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20
Q

Acid Fast stain

A

AFB grading national Std.

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22
Q

Dorset

A

Egg base

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23
Q

Result of HSCT

A

> 45mm height of gas bubbles

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25
Q

Middle brook 7H9

A

Liquid base

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26
Q

Positive for T 80 HOH

A

M. kansasii

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28
Q

Principle of Niacin Test

A

Niacin + Niacin Ribonucleotide + aniline dye + cyanogens bromide

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29
Q

Reduction positive

A

M. avium

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30
Q

+n

A

1-9 AFB per 100 fields

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32
Q

American thoracic society medium

A

Egg base

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34
Q

6% Oxalic Acid is for?

A

Specimen contaminated with Pseudomonas like urine and stool

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37
Other name of MB TB
Koch bacillus
38
specimen for nitrate reduction test
avium + | kansasii +
40
Anaerobe or aerobe?
Obligate AEROBE
42
Positive for TRT
M. avium
43
Best medium for NIACIN test
LJ medium
45
Anaerobic Non spore former Resist Acid Alcohol
true
47
Mitchison Medium
Agar base
48
2+
1-10 AFB per fields in at least 50 fields
50
Petragnani Medium
Egg base
52
3 Nitrate Reduction Test chemicals
HCl Sulfanilamide n-napthulethylene diamine
53
tween 80 hoh
kansasii | avium
54
For heavily contaminated specimens
Petragnani medium
55
Egg base Medium
Highly Proteinized | Don't Autoclave
56
Positive for HSCT
M. kansasii
57
hsct
kansasii | tb
58
Carbon source for LJ medium
Glycerin
59
arylsulfatase
fortuitum + | chelonei +
60
Gram Stain is for?
Decontamination/Digestion
61
Positive for NRT
M. avium
62
non specific to MTB
LJ medium
63
Ideal size of smear
2cm by 3
64
It produces what?
Much granules
65
Positive Niacin test
MTB
66
Negative Niacin Test
M. bovis
67
Meaning of NALC
N-Acetyl L-Cysteine
69
Semi quantitative
heat stable catalase test
70
What other spp. can grow on LJ medium?
Nocardia
71
Reagent for HSCT
30% H2O2
72
Medium for HSCT
Tween 80
73
No. 1 NTM in AIDS patients
M. avium
74
Niacin test
tb | bovis
75
Result of Tellurite Reduction Test
Black Metallic
76
Principle of Tween 80 HOH test
Tween 80 ---> HOH of tween 80
77
Result for NRT
Pink or Yellow
78
Result of Tween 80 HOH test
Red
79
Heat Stable Catalase Test
Tween 80 + MB + 30% H2O2 + 68 deg
80
Tween 80 positive
M. kansasii
82
Negative for TRT
M. kansasii
83
tellurite reduction test
avium kansasii
84
Principle of Tellurite reduction test
tellurite --> black metallic tellurium
85
% of NALC-NaOH
2 to 3%
86
3 groups of Mycobacterium
MB Tuberculosis Complex MOTT MB leprae
87
check in _____ fields before reporting
300
88
best for digestion
NALC NaOH + Na Citrate
89
For digestion
NALC NaOH + Na Citrate Zephiran Trisodium PO4 6% Oxalic Acid Dithiothreitol
92
Bottle number for CSF
2
93
Result of Niacin test
yellow
97
how to sterilize LJ medium?
Inspissation | 70 to 80 deg for 2 hours
101
temp for HSCT
68 deg
102
Nitrate to Nitrite
Nitrate reduction test
111
Cannot be grown on agar
MB leprae
112
Automated System
Liquid Media
114
Best with glycerin
LJ medium
115
Septi Check
Liquid base
117
Vaccine for TB
Bacillus Calmette Gwerin
124
Pulmonary TB
Tuberculosis | Africanum
135
Virulence for MB TB
Cord factor | Sulfatides
136
How to sterilize loop after using?
Dip in lysol then in sand
138
0
no AFB per 300 fields