MYCOLOGY SPECIMENS AND TESTS

Question 1

when should we collect viral spx?

Answer 1

early/acute phase (within 72 hours)

Question 2

viral swab material requirement

Answer 2

dacron fibers or nylon

Question 3

mortal sin in swab

Answer 3

calcium alginate, cotton, or wooden

Question 4

spx processing:

urine

Answer 4

neutralize w 7.5 calcium bicarbonate

Question 5

spx processing:
blood
volume:
ac:

Answer 5

3-5ml

heparin/citrate

Question 6

ac to be used for pcr methods

Answer 6

edta

Question 7

ac that inhibits polymerase

Answer 7

heparin

Question 8

viral transport media ph and indicator

Answer 8

7.2 phenol red

Question 9

antibiotics used in viral transport media

Answer 9

streptomycin and penicillin

Question 10

3 components of viral transport media

Answer 10

isotonic soln, protein, antibiotics

Question 11

virus processing temp and time requirement

Answer 11

4C, up to 5 days

Question 12

delay of 6 days or longer temp req

Answer 12

-20 or -70

Question 13

respiratory spx and virus (3)

Answer 13

throat swab - influenza
nasal washings - parainfluenza
nasopharyngeal aspirate - RSV

Question 14

gastroenteritis virus (2)

Answer 14

rectal swab - norwalk agent

stool - rotavirus

Question 15

skin lesion virus (2)

Answer 15

skin scrapings - VZV

vesicular fluid - HSV

Question 16

congenital infection virus

Answer 16

urine - CMV

Question 17

meningitis virus

Answer 17

blood and csf - togavirus

Question 18

virus that can be seen using light microscopy

Answer 18

pox virus

Question 19

inclusion bodies:

ca of negri bodies

Answer 19

rabies virus

Question 20

inclusion bodies:

ca of torres councilman bodies

Answer 20

yellow fever virus

Question 21

inclusion bodies:

ca of bollinger bodies

Answer 21

fowl pox virus

Question 22

inclusion bodies:

ca of guarneri paschen bodies

Answer 22

variola and varicella

Question 23

microscope used to detect the morphology of virus

Answer 23

electron microscopy

Question 24

indication of HPV infection

Answer 24

koilocytes

Question 25

smear that detects VZV or HSV inclusions

Answer 25

tzanck smear

Question 26

gold standard for virus detection

Answer 26

viral culture

Question 27

type of cell culture: | uses normal, mature adult cells

Answer 27

primary cell culture

Question 28

type of cell used in primary cell culture

Answer 28

rhesus monkey/rabbit kidney cells

Question 29

type of cell culture: | uses embryonic or fetal cells

Answer 29

diploid/semi continuous cell lines

Question 30

primary cell culture passage times

Answer 30

once or twice

Question 31

diploid cell lines passage times

Answer 31

50x

Question 32

type of cell used in diploid cell lines

Answer 32

fibroblasts from lung/foreskin

Question 33

diploid cell to isolate CMV

Answer 33

human embryonic fibroblast

Question 34

diploid cell to isolate VZV and HSV

Answer 34

human fetal diploid

Question 35

type of cell culture: | uses malignant or cancer cells

Answer 35

heteroploid/continuous cell line

Question 36

heteroploid passage times

Answer 36

unlimited

Question 37

heteroploid cell that uses cervical cancer cells

Answer 37

HeLa cells

Question 38

heteroploid cell that uses laryngeal cancer cells

Answer 38

Hep2 cells

Question 39

indication that there is a viral growth in cell cultur

Answer 39

cytopathic effect

Question 40

cytopathic effect examples

Answer 40

``` pyknosis fused cells vacuoles in cytoplasm swelling and clumping proliferation of nuclear membrane ```

Question 41

CPEs: | condensation/shrinking of nucleus

Answer 41

pyknosis

Question 42

virus that can cause pyknosis PYK PIC

Answer 42

picornavirus

Question 43

virus that can cause cell fusion

Answer 43

paramyxovirus

Question 44

virus that can cause vacuolation

Answer 44

polyomavirus and papillomavirus

Question 45

virus that can cause swelling and clumping

Answer 45

adenovirus

Question 46

virus that can cause nuclear membrane proliferation

Answer 46

alpha herpes virus

Question 47

this happens when there is no or little CPEs

Answer 47

hemadsorption

Question 48

virus that can cause hemadsorption

Answer 48

influenza, parainfluenza, mumps