Neisseria Spp. Flashcards
(119 cards)
1
Q
Which of the following is/are not a characteristics of Neisseria?
A. Nonmotile
B. Non-spore forming
C. Gram negative diplococci
D. All are catalase (+)
A
D. Not all are (+) some are negative like N.elongata and N. bacilliformis
2
Q
T or F
Neisseria spp. Are capnophilic.
A
True
3
Q
What is the natural habitat of Neisseria spp.?
A
Mucous membrane
4
Q
Other name of N. gonorrhoeae and
N. meningitidis
A
-Gonococci
-Meningococci
5
Q
They are the primary human pathogens of the Neisseria spp.
A
N. gonorrhoeae
N. meningitidis
6
Q
T or F
N. gonorrhoeae can be normal flora in the body.
A
False- they are always pathogenic
7
Q
Neisseria sp that is usually found as a commensal inhabitant of the upper respiratory tract but can also become invasive
A
N. meningitidis
8
Q
Nesseria spp. are fastidious, and they grow best in what media?
A
Enriched media (CHOC)
9
Q
Neisseria require ___ in ____for growth.
A
Iron in transferrin.
10
Q
What are the virulence factors of Neisseria spp?
A
- Receptors for human transferrin
- Capsule
- Pili
- cell membrane protein
- lipooligosaccharide or endotoxin
-IgA protease
11
Q
Colony type of N. gonorrhoeae that has pili.
A
T1 and T2
12
Q
Colony type of Neisseria that doesn’t have pil
A
T3 to T5
13
Q
Neisseria type that is more virulent.
A
T1 and T2
14
Q
Cell membrane protein that si found in the surface of bacterial cells
A
Por (porin) protein 1
15
Q
Two type of Por protein of Neisseria.
A
PorA
PorB
16
Q
Por gene that onlyfound in N. gonorrhoeae
A
PorB
17
Q
T or F
porA and porB gene are both found in N. meningitidis
A
True
18
Q
Also known as protein 2
A
Opa (opacity)
19
Q
Type of cell membrane protein of Neisseria for the adherence to phagocytic and epithelial cell
A
Protein 2 (Opa)
20
Q
Also known as the Protein 3.
A
Reduction modified protein (Rmp)
21
Q
This cell membrane protein of Neisseria blocks host serum bactericidal action (IgG)
A
Rmp protein (protein 3)
22
Q
Major in vivo virulence factors that mediates damage to body tissues and elicits inflammatory response
A
Lipooligosaccharide (LOS)/ Endotoxin
23
Q
LOS damage tissue by ___.
A
Blebs
24
Q
Only natural host of N. gonorrhoeae.
A
Human
25
An acute pyogenic infection of non-ciliated columnar and transitional epithelium
Gonorrhea
26
Primary reservoir of N. gonorrhoeae.
Asymptomatic carriers
27
Gonorrhea in men is usually ___, and in women are ___.
Men: Acute urethritis
Women: endocervix
28
(Men) resulting in purulent discharge and dysuria
Acute urethritis
29
Complication of gonorrhea in men are ___.
Prostatitis and epididymitis
30
(Women) cervical discharge, dysuria, lower abdominal pain.
Endocervix
31
Common complications of gonorrhea in women.
Pelvic inflammatory disease (PID)
Fitz-Hugh-Curtis syndrome
32
Complication of Gonorrhea causes by sterility, ectopic pregnancy or perihepatitis
Fitz-Hugh-Curtis syndrome
33
Blood-borne dissemination of N. gonorrhoeae results in ___.
Purulent arthritits
34
The gonorrhea infection is common in homosexual intercoarse, especially with men.
Anorectal of Oropharyngeal infections
35
Gonorrhea infections tha infect the baby.
Opthalmia neonatorum
36
Specimens for diagnosing Gonorrhea.
Genital sites
Rectum
Pharynx
Joint fluid
37
Specimen of choice for Gonorrhea infection.
Men: urethra (pus)
Female: endocervix
38
Preferred swab for collecting N. gonorrhoeae.
Dacron or rayon swabs
39
T or F
N. gonorrhoeae is resistant to drying and temperature changes.
False -susceptible
40
What are the transport system for N. gonorrhoeae?
JEMBEC plates
Gono-pak
Transgrow
Amies with charcoal
41
The swab containing the N. gonorrhoeae should be rolled in ____.
Z pattern.
42
Shape of N. gonorrhoeae under the microscope
Kidney/coffee bean shaped
43
Thayer-Martin Inhibitory agents.
Vancomycin
Colistin
Nystatin
44
What inhibitory agent was added in Modified Thayer-Martin?
Trimethoprim
45
Modified Thayer-Martin
Vancomycin:
Colistin:
Nystatin:
Trimethoprim:
Gram (+)
Gram (-)
Yeast
Swarming proteus soo.
46
In Martin-Lewis, what are the Inhibitory reagent?
Vancomycin
Colistin
Anisomycin
Trimethoprim
47
In Martin-Lewis what is the inhibitory reagent for yeast?
Anisomycin
48
What do you call the modified Martin-Lewis?
New York City
49
What inhibitory agent was modified in New York City?
Amphotericin B
50
In New York City medium, what is the inhibitory agent for yeast?
Amphotericin B
51
What are the inhibitory agents in GC-LECT medium?
Vancomycin
Lincomycin
Colistin
Amphotericin B
Trimethoprim
52
In GC-LECT, what inhibitory agent is for gram (+)?
Lincomycin
Vancomycin
53
Inoculated plates of N. gonorrhoeae should be incubated at what temp. And %of CO2?
At 35°C
3-5% CO2
54
What is used for incubation of N
gonorrhoeae?
Candle extinction jar or CO2 incubator
55
Which among is not the characteristics of N. gonorrhoeae colony morphology?
A. Small
B. Grey-tan
C. Translucent
D. Tenacious
E. Raised
D
56
Characteristics of T1 and T2 colonies.
Small
Raised
Translucent
Brighter
Tan
57
Characteristics of T3-T5 colonies.
Large
Flat
Dryer
58
They produce smaller colonies and grow more slowly compare to N. gonorrhoeae.
Arginine Hypoxanthine Uracil (AHU)
59
Appearance of N. gonorrhoeae
In oxidase test.
Purple
60
Color of colony of N. gonorrhoeae when an oxidizing agent is added directly to the colonies
Deep purple -black
61
Traditional method in identifying Neisseria spp.
Carbohydrate Utilization
62
In traditional method in identifying the N. gonorrhoeae, what media is used?
Cystine trypticase Agar
63
The CTA in Identifying N. gonorrhoeae contains what?
1% carbohydrate
Phenol red (pH indicator)
64
N. gonorrhoeae only ferment what CHO?
Glucose
65
It is used to detect enzymes that hydrolyze colorless substrates and produce colored end products.
Chromogenic substrates
66
The advantage of Chromogenic substrates.
Identification of strains of N. gonorrhoeae
67
Method employs monoclonal antibodies for identification of N. gonorrhoeae.
Immunologic Assays
68
Modification of catalase test (auxotypes).
Superoxol (30% H2O2)
69
Most common auxotype
AHU strain
70
Antimicrobial resistance of N. gonorrhoeae.
PPNG -Plasmid-mediated penicillinase-producing Neisseria gonorrhoeae.
CMRNG- Chromosome-mediated penicillin resistance to both penicillin & tetracycline
71
Treatment for Gonorrhea.
Cephalosporins (ceftriaxone & cefixime)
72
N. meningitidis are important etiologic agent of endemic and epidemic of __.
Meningitis
Meningococcemia
73
N. meningitidis are normal foud usually in __.
Mucous membrane of nasopharynx and oropharynx
74
T or F
por A and por B are both present in N. meningitidis.
True
75
Route of transmission for N. meningitidis.
Oral secretion/Respiratory droplets
76
When N. meningitidis enters the bloodstream, and two main diseases can occur. What are they?
Fulminant meningococcemia (deadly)
Meningitis
77
Meningococcemia or sepsis may develop what?
- Purpura with petechial skin rash
- tachycardia
- hypotension
-thrombosis (common)
78
Fulminant meningococcemia can develop what?
- disseminated intravascular coagulation (DIC)
- septic shock
- hemorrhage in the adrenal glands (Waterhouse-Friderichsen syndrome)
79
Individual with deficiency of complement components ___ are at risk of of meningococcemia.
C5 to C8
80
What serogroup usually cause Meningococcal pneumonia?
Serogroup Y
81
What specimens can N. meningitidis recover?
CSF
Blood
Nasopharyngeal swabs or aspirate
Joint fluid
Sputum and urugenital sites (less common)
82
Apperance of N. meningitidis under the microscope.
Intracellular and Extracellular gram(-) diplococci
83
Media for N. meningitidis.
SBA
CHOC (ordinary can be used)
84
Appearance of N. meningitidis in SBA.
Bluish gray colonies
85
Appearance of N. meningitidis in CHOC.
Tan, small, sometimes mucoid, convex
86
Which is/are not true about N. meningitidis?
A. Catalase (+)
B. Oxidatice (+)
C. Utilize maltose and glucose
D. Utilize fructose and glucose
D
87
A nonpathogenic that mimics the N. meningitidis.
N. lactamica
88
Drug of choice for meningitis.
Penicillin
89
Drug of choice for profilaxis for close contact to N. meningitidis.
Rifampin
Sulfunamides
90
A polysaccharide-protein conjugated vaccine for N. meningitidis.
Menactra vaccine
91
M. catarrhalis belongs to family of ____, which contains 3 genera: ____.
Moraxellaceae
Moraxella
Acinetobacter
Psychrobacter
92
They are commensal of upper respiratory tract.
M. catarrhalis
93
M. catarrhalis can cause ___, especially in adults with COPD
Lower respiratory tract infection
94
Usual URT infection cause by M. catarrhalis.
Common cold
95
Identify the common cause of acute otitis media and sinusitis in children in order.
1st- S. pneumoniae
2nd- Haemophilus influenzae
3rd- M. catarrhalis
96
Which is/are not true about M. catarrhalis?
A. They grow in SBA and CHOC
B. smooth
C. Shiny
D. Gray to white
E. Convex
C. And E
97
It has been used to describe the colonies of M. catarrhalis because it remains intact when pushed across the plate with loops.
Hockey pucks
98
Appearance of old colonies of M. catarrhalis.
Wagon-wheel appearance
99
T or F
M. catarrhalis can grow in high temperature.
False- low temperature and grows well at 28°C
100
What sugar does M. catarrhalis ferment?
They are ASACCHAROLYTIC (don't ferment sugar)
101
Which is not true about biochem test for N. gonorrhoeae?
A. DNase (+)
B. Butyrate (-)
C. Butyrate (+)
D. Catalase (+)
E. Oxiase (-)
B and E
102
What group of N. Spp. Are traditional pathogens.
Group 1
103
Group of Neisseria spp. That can grow on selective media.
Group 2
104
Group of commensal Neisseria spp. That not usually grow in selective media
Group 3
105
Group 2 and 3 Neissera are subidvided further, what are they?
Sacchrolytic
Asacchrolytic
106
They are misidentified as N. gonorrhoeae
N. cinerea
107
T or F
N. cinerea is susceptible in Colistin
True
108
They are yellow pigmented
Asacchrolytic
Grows in SBA and CHOC at 22°C
109
They are found in nasopharynx of children.
N. lactamica
110
Misidentified as N. meningitidis.
They are slightly smaller, and they are lactose fermenter.
N. Lactamica
111
This N. spp. Are ONPG (+)
N. lactamica
112
Large colonies and normal flora of dolphins.
N. mucosa.
113
Misidentified as N. Meningitidis
-they release large amount of Extracellular polysaccharide when grows in media that contains 1-5% sucrose.
N. polysaccharea
114
The colonies are dry, wrinkled, adherent, and breadcrumbs like.
N. sicca
115
N. spp. able to ferment sucrose amd fructose.
N. sicca
116
Two most common N. spp. Foun in RT of Adult
N. sicca
N. Subflava
117
They are less yellow Neisseria sp. And grows at 22°C.
N. subflava
118
3 Neisseria spp. That is rod-shaped
N. elongata
N. nitroreducens
N. glycolytica
119
Rod shape and normal flora of dogs.
N. wequeri
