NETS

Question 1

What is a NET

Answer 1

Neutrophil extracellular trap
DNA web like structure with highly toxic proteins, circulate through blood to control pathogens

Question 2

Harmful impacts of NETS

Answer 2

Can drive microvascular thrombosis
Inflammation
Cytotoxicity
Tumour microenvironment - can cover cancers helping cancer avoid immune system
Autoimmunity - may be involved in infections triggering autoimmune conditions
Protein aggregation

Question 3

Nucleocapture

Answer 3

Blood purification technique using histones to remove cfDNA/NETs
Can be added to ECMO or dialysis
Single pass clears 95%+ of NETs

Question 4

Nucleocapture indications

Answer 4

Severe sepsis
Primary graft dysfunction
Reconditioning poor quality liver transplant
Lupus
Alzheimer’s