non-fermentative gram negative bacilli Flashcards
unit 5 (47 cards)
non-fermentative gram-negative bacilli are considered as what kind of pathogens?
opportunistic
habitat non-fermentative gram negative bacilli
water, soil, vegetables, food, and hospital surface
non-fermentative gram negative bacilli can withstand what disinfectants?
chlorhexidine
they oxidize carbohydrates to derive energy for their metabolism
oxidizers
do not break down carbohydrates at all
nonoxidizers
why is acid production absent in oxidative-fermentative (OF) media?
they are oxidative
two ways on how bacteria utilizes carbohydrates
fermentation and oxidation
bacteria can utilize carbohydrates with or without oxygen
fermentation
bacteria require oxygen
oxidation
meaning of OF test
oxidative-fermentative test
determines the ability of organisms to utilize a substrate
oxidative-fermentative test
positive result of OF test in an open tube
exhibit a yellow color
positive result of OF test in a closed tube
exhibits a green/blue-green color
it is a medium of OF test with 1& carbohydrate and bromothymol blue as pH indicator
Hugh and Leifson medium
it is a standardized system for the identification of non-fastidious, non-enteric Gram-negative rods
API 20NE
provides automated approaches to the identification of a variety of bacteria and yeasts
VITEK-2 Compact
a method for the identification of bacteria and fungi based on the analysis of unique spectra of their peptides
MALDI-TOF MS
vigorous-fast swimming bacterium as seen in samples from the wild or from patients
I.pseudomonas
I.pseudomonas is a major pathogen among patients with what condition?
cystic fibrosis
two classifications of pseudomonas
palleroni and kersters
most commonly isolated species
Pseudomonas aeruginosa
leading cause of nosocomial respiratory tract infections
Pseudomonas aeruginosa
it is the most important virulence factor of P.aeruginosa
exotoxin a
a blue green pigment, produced only by P.aeruginosa
pyocyanin