Non-genetic analysis Flashcards Preview

Cell and molecular biology > Non-genetic analysis > Flashcards

Flashcards in Non-genetic analysis Deck (5)
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1
Q

How can you detect where proteins are in the cell?

A

Tagged antibodies

2
Q

How can you detect antibodies?

A

Fluorescent tags- detected via a fluorescent microscope/ photodetector
Enzyme conjugates-
alkaline phosphatase- substrate turns blue
horseradish peroxidase- substrate turns brown

3
Q

What is the purpose of a ‘antibody sandwich’?

A

Secondary antibodies- amplify the signal- because multiple secondary antibodies can bind to a primary antibody

4
Q

What is an antisense probe?

A

Antisense (synthesised from cdna) probe hybridises with complementary mRNA- blocks its translation

5
Q

What are the uses for a GFP transgenic line?

A
  1. To follow the expression of a gene in the whole animal
  2. To follow the sub-cellular localisation of a protein
  3. To follow the behaviour of cells- marked cells can be distinguished from others