Nucleic Acid Extraction

Question 1

Enzyme for denaturation

Answer 1

Proteinase K

Question 2

Organic Extraction Reagents

CLUE: PCIA

Answer 2

PCIA: Phenol, Chloroform, Isoamyl Alcohol

Question 3

Nucleic Acid extraction in: Inorganic extraction, what kind of technique?

CLUE: Salt

Answer 3

Salting out technique

Question 4

what is the charge of DNA. positive or negative?

Answer 4

Negatively Charge

Question 5

Nucleic Acid absorbance

Answer 5

260 nm

Question 6

Phenol Absorbance

Answer 6

230 nm

Question 7

Protein Absorbance

Answer 7

280 nm

Question 8

secondary measure of euclid acid purity

(CLUE- __/__ Ratio)

Answer 8

260/230 Ratio

Question 9

Guanidine isothiacyanate Absorbance

(clue: same absorbance w/ nucleic acid)

Answer 9

260 nm

Question 10

What technology uses in QUBIT Fluorometer?

(choices: Flurorescence or UV absorbance)

Answer 10

Fluorescence

Question 11

What technology uses in QUBIT Nano spectrophotometry?

Answer 11

UV absorbance

Question 12

Primer used
(Clue: starts with letter O)

Answer 12

Oligonucleotide

Question 13

building blocks of PCR

Answer 13

dNTP

Question 14

Type of PCR: Generate a temperature gradient across the heating block for the annealing step.

Clue- “gradient”

Answer 14

Gradient PCR

Question 15

Strong sharp band in agarose gel electrophoresis indicates:

a. uniform size & sufficient quantity DNA
b. Fragmentation of DNA Sample

Answer 15

a. uniform size & sufficient quantity DNA

Question 16

Smear agarose gel electrophoresis indicates:

Answer 16

a. uniform size & sufficient quantity DNNA
b. Fragmentation of DNA Sample

Question 17

Electrophoresis: what is the meaning of PAGE acronym

Answer 17

Polyacrylamide gel electrophoresis

Question 18

also known as vertical electrophoresis

Answer 18

Polyacrylamide gel electrophoresis