Nucleic Acids Flashcards
(18 cards)
Which base pair forms 3 hydrogen bonds
G-C
Can RNA form base pairs
Yes
G-C AND A-I
What are the two ends of the DNA molecule ferried to as
3’ and 5’
What is a nucleotide made up of
Sugar molecule, phosphate group and heterocyclic base
How is a DNA molecule charged at physiological conditions - ph7
Main contributor to change is the phosphate group
-1
How stop codons are in the standard genetic code
3 stop condons
Which nuclei bases in DNA have aromatic character
All of them!
Why is RNA less stable than DNA
As RNA has an -OH group at C2 of the ribose rugar, this is ready to react with the phosphate group in C3
Vs oxygen just has H
What is the bond between the sugar and nucleobase referred to as
N-glycosidic bond
Reverse complement of 5’ TTAGCTGTTG 3’
5’ CAACAGCTAA 3’
Which nucelobases are purine derivatives
Adeline and guanine
How is RNA charged at physiological conditions eg PH7
Phosphate group has a negative charge
-1
What is meant by the term isoelectric point
The pH at which all the charges of the amino acid in the peptide chain cancel out and equal 0
Including at the N- and C- termini as well as side chains
So it wont move in an electric field
Which amino acid would a radioactive sulphur be found
Cystine and Methionine
Which protein organic amino acids contain charged side groups
HKR are positive
DE are negative
If the pka for the N-terminus of an amino acid is 9.87, and the pka for the C-terminus is 2.35, w no charged side groups. What’s the isoeletric point
N+C / 2
6.11
How would the charge of alanine change if the pH was increased
It would be negatively charged
What’s the rough pka for an amino acid of a proteiogeic (utilised in the bisynthesis of protien)amino acid
8.5-10.5
These amino acids are H, ISOlucine, leucine,lysice , m , ph, theronine, tryptophanophan ands valine
