OECD Guidelines

Question 1

What is the preferred species for acute dermal irritation/corrosion testing?

Answer 1

Rabbit

Question 2

In the Acute Dermal Irritation/Corrosion test, if a corrosive effect is not observed after the last patch is removed, how long should the animal be observed?

Answer 2

14 days, unless corrosion develops at an earlier time point.

Question 3

What is the preferred species for acute eye irritation/corrosion testing?

Answer 3

Rabbit

Question 4

During the Daphnia acute immobilization test, what percent of daphnids should have been immobilized? What is being evaluated?

Answer 4

< 10%; EC50

Question 5

During the Daphnia acute immobilization test, how long are young daphnids exposed to a chemical?

Answer 5

48 hours

Question 6

During the Daphnia acute immobilization test, what should the dissolved oxygen concentration be at the end of the test in the control and test vessels?

Answer 6

> 3mg/L

Question 7

What is the species of the preferred Daphnia species in the OECD Daphnia Acute Immobilization test?

Answer 7

Daphnia magna Straus is preferred, although others may be used (Daphnia pulex)

Question 8

What is an EC50?

Answer 8

Half Maximal EFFECTIVE CONCENTRATION. It’s a measurement of the concentration of a drug, antibody, or toxicant which induces a biological response halfway between baseline and maximum after a specified exposure time.

Question 9

What is being evaluated in the Acute Fish Toxicity Test?

Answer 9

LC50

Question 10

What is an LC50

Answer 10

The median lethal concentration (50%)

Question 11

What is the study duration of the Acute Fish Toxicity Test

Answer 11

Fish are exposed to a chemical for 96 hours.

Question 12

What is the maximum loading for fish in the Fish acute toxicity test?

Answer 12

A loading not exceeding 5g/L of solution at any time.
A maximum of 0.8g wet weight fish/L for fresh water static and semi-static.
0.5g wet weight fish/L per 24 hours for flow through systems.

Question 13

What is the minimum number of fish that must be used at each test concentration and in the controls for the Acute Fish Toxicity Test?

Answer 13

7

Question 14

True or False:
In the Fish Acute Toxicity Test- Surviving fish of the treatment groups are euthanized at the end of the exposure, whereas euthanasia of surviving control fish is not required, but they should not be used in another test.

Answer 14

True

Question 15

What is being evaluated in the earthworm acute toxicity test?

Answer 15

LC50. (concentration that kills 50% of test animals in a test period)
Mortality in controls should not exceed 10%.

Question 16

Describe the two principle test methods of the earthworm acute toxicity test.

Answer 16

Screening test (filter paper contact test) involves exposing earthworms to test substances on moist filter paper to identify potentially toxic chemicals to earthworms in soil.

Artificial soil test involves keeping earthworms in samples of defined artificial soil with a range of test substance that was applied. Mortality is assessed 7 and 14 days after application.