OFT Flashcards
1
Q
Factors affecting OFT
A
Shape of RBC
VOlume of surface area of RBC
Functional state of RBC membrane
2
Q
how many tubes needed
A
12 tubes
3
Q
Help diagnose different types of anemia
A
Osmotic Fragility test
4
Q
Initial hemolysis
A
not so compact sedimentation and faint pink solution
5
Q
no hemolysis
A
compact sediment and clear solution
6
Q
how to get 0.5% solution
A
Sodium chloride (0.50g) + Distilled water (100ml)
7
Q
Sources of errors
A
Traumatic blood collection Dilution not accurate Temperature and pH not constant Bacterial contamination Not chemically pure saline solution
8
Q
Precautionary measures
A
avoid Hemolysis
9
Q
Congenital Spherocytosis
Ovalocytosis
Elliptocytosis
Hemolytic anemia
A
Increase OFT
10
Q
IDA Liver disease Sickle cell anemia Presence of target cell Splenectomy
A
Decrease OFT
