Oncogenes and tumour suppressor genes

Question 1

What were the original 6 hallmarks of cancer

Answer 1

Sustaining proliferative signalling
Resisting cell death
Inducing angiogenesis
Enabling replicative immortality
Activating invasion and metastasis
Evading growth suppressors.

Question 2

What are the emerging and enabling hallmarks

Answer 2

Emerging:
Deregulating cellular energetics- switch to the more energy dependent anaerobic respiration
Avoiding immune destruction.

Enabling:
Genome instability and mutation- to promote mutagenesis that leads to aberrant growth
Tumour-promoting infiltration.

Question 3

Summarise the key features of the cell cycle

Answer 3

Cycle checkpoints (growth arrest ensures genetic fidelity).
Specific proteins accumulate/ are destroyed during the cycle.
Cyclins, cycle dependent kinases, cycle dependent kinase inhibitors
Permanent activation of a cyclin can drive a cell through a checkpoint.

Question 4

Describe the G1-S checkpoint

Answer 4

Check for DNA damage

Restriction point: check for cell size and favourable environmental conditions (growth factor)

Question 5

Describe the G2-M checkpoint

Answer 5

Check for damage or unduplicated DNA

Question 6

Describe the metaphase checkpoint

Answer 6

Check for chromosome attachment to mitotic spindle.

Question 7

What happens at these checkpoints

Answer 7

Cell cycle is arrested
If DNA damaged- tries to repair with DNA repair genes e.g BRCA
If damage too big- apoptosis.

Question 8

Describe what is meant by proto-oncogenes

Answer 8

Proto-oncogenes code for essential proteins involved in maintenance of cell growth, division and differentiation.
Mutation converts a proto-oncogene to an oncogene, whose protein product no longer responds to control influences.
Oncogenes can be aberrantly expressed, over-expressed or aberrantly active.
E.g. MYC, RAS, ERB, SIS
Proto-oncogenes can be converted to an oncogene by a single mutation.

Question 9

What is the key difference between photo-oncogenes and oncogenes

Answer 9

Proto-oncogene: code for essential proteins involved in maintenance of cell growth, division and differentiation e.g. Kinases, phosphorylases, transcription factors

Oncogene: mutations in proto-oncogenes that promote uncontrolled cell proliferation - aberrantly expressed, over-expressed (e.g. HER2) or aberrantly active

Question 10

Describe how normal photo-oncogenes can be activated

Answer 10

The normal proto-oncogene can be activated in 4 ways:
1. Mutation in the coding sequence.
 Point mutation or deletion.
2. Gene amplification.
 A protein may block the DNA polymerase so the polymerase repeatedly backs up to go over the area a few times creating many identical genes.
3. Chromosomal translocation.
 Chimeric genes.
4. Insertional mutagenesis.
 Viral infections – some viruses insert their genome into our DNA and usually this isn’t a problem as much of our DNA does not code but if it’s in a coding region, this could be cancer.

Question 11

What is the end result of gene amplification

Answer 11

You get multiple copies of the protein produced.

Problem with HER2 in Breast Cancers

Question 12

What are Chimeric genes

Answer 12

Genes that are formed by combinations of portions of one or more coding sequence to produce new genes (e.g. the swapping of tips of chromosomes)

Question 13

When can the formation of chimeric genes be problematic

Answer 13

If one of the pieces of translocated DNA is a promoter, it could lead to upregulation of the other gene portion (this occurs in Burkitt’s lymphoma)
If the fusion gene codes for an abnormal protein that promotes cancer

Question 14

What is the Philadelphia chromosome

Answer 14

Chromosome produced by the translocation of the ABL gene on chromosome 9 to the BCR gene on chromosome 22
The BCR-ABL fusion gene encodes a protein that promotes the development of cancer

BCR is anti-apoptotic- so enables tumour to be resistance to stop signals.

Question 15

Describe the two possible results of chromosomal translocation and insertional mutagenesis

Answer 15

Strong enhancer increases normal protein levels
e.g. Burkitt’s lymphoma- lose normal regulation of gene

Fusion to actively transcribed gene overproduces protein or fusion protein is hyperactive.
e.g. Philadelphia chromosome

Question 16

Summarise the Philadelphia chromosome

Answer 16

Philadelphia chromosome: end of long arms of Chr9 and Chr22 exchanged to form the BCR-ABL fusion protein - ABL is a very strong promotor region

Question 17

Summarise the signal transduction pathways

Answer 17

Steroid hormone- Nuclear or cytosolic receptor — transcription/translation — proliferation

Tyrosine Kinase receptor — ligand binds — phosphorylation — proliferation

GPCR- kinase- phosphorylation – proliferaation

Proteins involved in signal transduction are potential critical gene targets (proto-oncogenes)

Activation of proto-oncogenes to oncogenes disrupts normal activity

Question 18

Give some examples of signal transduction proteins that are photo-oncogenes

Answer 18

 Signal transduction proteins are proto-oncogenes.
 Examples:
o Tyrosine kinase receptors EC – met, neu.
o Tyrosine kinase receptors IC – src, ret.
o Transcription factors – myc, fos, jun.
o GPCR g-proteins – ras, gip-2.
o Kinases – raf, pim-1.

May habe a point mutation which changes conformation - so inhibitory proteins cannot bind
or changes phosphorylation residues- such that it’s constitutively active.

The higher up the pathway the mutation is- the harder it is to treat- due to a greater dysregulaiton of downstream pathways.

Question 19

Describe the aberrant activity of mutant Ras

Answer 19

Upon binding GTP, RAS becomes active.
Dephosphorylation of the GTP to GDP switches RAS off.
Mutant RAS fails to dephosphorylate GTP and remains active.

Leading to cell proliferation independent of growth signals

Question 20

Describe some photo-oncogenes and their associated cancers

Answer 20

SRC
Tyrosine Kinase
Overexpression/ C-terminal deletion
Breast/colon/lung

MYC
TF
Translocation
Burkitt’s lymphoma

JUN
TF
Overexpression/deletion
Lung

Ha-RAS
G-protein
Point mutaation
Bladder

Ki-RAS
G-protein
Point mutation
Colon/lung

Question 21

Describe Ras signalling

Answer 21

o The binding of GTP allows RAS to bind RAF and pass the signal to RAF deliver the signal further to MEK and ERK.

Phosphorylation of Tfs (myc. jun, fos)
Translocation to the nucleus
increasing RNA synthesis and transcription and translation of effector proteins which lead to cell proliferation and cell survival.

Question 22

Summarise tumour suppressor genes

Answer 22

Typically proteins whose function is to regulate cellular proliferation, maintain cell integrity.
E.g. RB
Each cell has two copies of each tumour suppressor gene- except in haploinsufficiency- where one mutation is sufficient to produce a tumour
Mutation or deletion of one gene copy is usually insufficient to promote cancer.
Mutation or loss of both copies means loss of control.

Question 23

Describe Knudson’s two hit hypothesis

Answer 23

Sporadic cancer : 2 acquired mutations in TSG

Hereditary cancer: 1 inherited and 1 acquired mutation- increased risk of cancer- already have 1 hit

Question 24

Summarise the inherited cancer susceptibility

Answer 24

Family history of related cancers.
Unusually early age of onset.
Bilateral tumours in paired organs.
Synchronous or successive tumours.
Tumours in different organ systems in same individual.
Mutation inherited through the germline.

Question 25

Describe the key features of retinoblastoma

Answer 25

Malignant cancer of developing retinal cells. Sporadic disease usually involves one eye. Hereditary cases can be unilateral or bilateral and multifocal. Due to mutation of the RB1 tumour suppressor gene on chromosome 13q14. RB1 encodes a nuclear protein that is involved in the regulation of the cell cycle. RB1 ensures progression of cell-cycle progression fro G1 -- S

Question 26

Describe the functional classes of tumour suppressor genes

Answer 26

``` Regulate cell proliferation Maintain cellular integrity Regulate cell growth Regulate the cell cycle Nuclear transcription factors DNA repair proteins Cell adhesion molecules Cell death regulators ``` All with the aim of: Suppress the neoplastic phenotype

Question 27

What is important to remember about tumour suppressor genes

Answer 27

A lot are lethal to the embryo

Question 28

Describe some examples of tumour suppressor genes

Answer 28

P53 Cell cycle regulation Many BRCA1 Cell cycle regulation Breast/ovarian/prostate Singl strand break repair, if overwhelmed- double strand break repair (homologous recombination) PTEN Tyrosine and lipid phosphotase Prostate/glioblastoma and Cowdens syndrome APC Cell signalling Colon p16INK4A Cell cycle regulation Colon MLH1 Mismatch repair Colon, gastric

Question 29

Summarise how a cell responds to DNA damage and cellular stress

Answer 29

``` Detect cellular insult and then switch on genes to repair the cell. Cellular stresses can include: Oxidative stress Nitric Oxide Hypoxia Ribonucleotide depletion mitotic apparatus dysfunction oncogene activation DNA replication stress Double-strand breaks Telomere erosion ``` These insults are sensed by the tumour suppressor gene p53 P53 is normally inactivated by a partner protein called MDM2 However, these insults cause MDM2 to be lost, activating p53 and allowing it to act as a transcription factor to turn on gene expression for genes involved in DNA repair

Question 30

Describe the p53 mediated response to mild/physiological stress and severe stress

Answer 30

``` Mild- regulation of p53 mediated genes involved in: metabolic homeostasis antioxidant defence DNA repair growth arrest ``` Severe stress- p53 mediated protein-protein interactions which lead to apoptosis.

Question 31

How many mutations are needed in p53 to get dysregulation of activity

Answer 31

Although p53 is a tumour supressor gene, mutants of p53 act in a dominant manner and mutation of a single copy is sufficient to get dysregulation of activity.

Question 32

Why is p53 hard to target in therapy

Answer 32

So many ubiquitous roles- would lead to plethora of side effects Important in healthy cells- side effects Molecular profiling of tumours to look for mutations - p53 may be driver mutations or passenger mutations- can't target p53 to one its functions.

Question 33

describe the importance of p53 mutations in cancer

Answer 33

Associated with poor prognosis and family histories of sarcomas and rare tumours.

Question 34

Describe the role of the APC tumour suppressor gene in familial adenomatous polyposis coli

Answer 34

Due to a deletion in 5q21 resulting in loss of APC gene (tumour suppressor gene). Involved in cell adhesion and signaling- MAPK Sufferers develop multiple benign adenomatous polyps of the colon. There is a 90% risk of developing colorectal carcinoma- prophylactic colonectomy and regular colonoscopies from teen years Adenomatous polyposis coli protein

Question 35

What signalling pathway is APC involved in

Answer 35

The tumour suppressor gene APC participates in the WNT signalling pathway. APC protein is a negative regulator of b-catenin, thereby preventing uncontrolled cell division. Mutation of APC is a frequent event in colon cancer. Wnt has a stimulatory effect on beta-cantenin

Question 36

Summarise the APC TSG

Answer 36

APC TSG: mutation of APC gene causing uncontrolled growth creating polyps, each with increased risk of cancer - becomes almost inevitable Normal function is to degrade beta catenin (WNT pathway - normally bound to cadherin in adherens junctions) Mutant APC leads to buildup of beta catenin, leading to binding to LEF-1 LEF-1: complex causes gene transcription

Question 37

What are two features of a normal healthy state

Answer 37

Proto-oncogene | Tumour suppressor gene

Question 38

Describe the different combinations that can lead to cell growth and proliferation (uncontrolled) and thus cancer

Answer 38

 Cancer can be triggered by: o Oncogene + TSG. o Proto-oncogene + defective TSG. o Oncogene + defective TSG.

Question 39

Describe an analogy that summarises the pathogenesis of cancers

Answer 39

Normal genes (regulated cell growth)- TSGs and port-oncogenes in check- car travels at steady speed 1st mutation (susceptible to cancer)- active oncogenes accelerating car- but TSGs acting as brake to halt progression towards cancer 2nd mutation or loss of TSG- accelerates to cancer

Question 40

Outline the development of colorectal cancer

Answer 40

Normal epithelium- Apc mutation - leads to hyper proliferative epithelium This increases the rate of mutations and likelihood go genomic instability: DNA hypomethylation and k-RAS MUTATION leading to adenoma p53 mutation then forms carcinoma which can then metastasise Hyperplasia  Metaplasia  Dysplasia  Carinogenesis

Question 41

What are the two main functions of b-cantenin

Answer 41

Functions in cell-cell adhesion (1 mark) Regulates transcription / gene expression (1 mark)

Question 42

Which protein, defective in familial adenomatous polyposis (FAP), regulates levels of b-catenin by controlling its degradation?

Answer 42

The adenomatous polyposis coli protein (1 mark) | 0.5 mark for APC

Question 43

Summarise Oncogenes

Answer 43

Oncogene Active in tumours Translocations/point mutations Not inherited Dominant Leukaemia/lymphoma Broad tissue specificity

Question 44

Summarise TSGs

Answer 44

Oncogene TSG Inactive in tumours Deletions/mutations Inherited Recessive Solid tumours Tissue specificity

Question 45

Describe the number of mutations needed for each cancer type

Answer 45

``` Colon- 11 Kidney- 2 Stomach- 4 Lungs- 6 Breast- 4 Brain -6 ```

Question 46

What is important to remember about cancer

Answer 46

Human cancer involves damage to DNA, or inheritance of aberrant sequences, at critical gene targets. These targets, proto-oncogenes and tumour suppressor genes, regulate cell cycle decisions (mitosis, arrest, differentiation, apoptosis). The ‘guardian of the genome’, p53 is a key player in decision making during the cell cycle. Studies of rare heritable cancers have led to an understanding of tumour suppressor genes. Colon cancer is a model for many of these factors.

Question 47

Summarise the genetic mutations that can cause cancer

Answer 47

Genetic mutations causing cancer: Chromosome translocation Gene amplification Point mutations within promotor or enhancer regions of genes Deletions or insertions Epigenetic alterations to gene expression