Organisation Flashcards
(170 cards)
Cells
Basic building blocks that make up all living organisms
Tissue
Group of similar cells that work together to carry out a particular function
Organ
Group of different tissues that work together to perform a particular function
Organ system
Group of organs working together to perform a particular function
Why can’t temperature be raised to speed up bodily reactions
- would speed up both useful and unwanted reactions
- cells would be damaged if temperature raised too high
Enzyme
Biological catalyst which increases the speed of a useful chemical reactions in the body without being changed or used up
How do enzymes get unique shape
- they are large proteins so made up of chains of amino acids
- chains folded into unique shapes
Active site
Enzyme’s uniquely shaped area where only substance involved in reaction binds
Induced fit enzyme model
Active site slightly changes shape as the substrate binds to get a tighter fit
Effect of temperature on enzymes
- higher temperature increases rate of reaction
- too high temperature breaks some bonds holding enzyme together, causing active site shape to change and substrate not fit
What does optimum temperature mean
Temperature which enzymes work best at
How does PH affect enzymes
Too high or low PH interferes with bonds holding enzyme together, changing shape of active site so substrate no longer fits
Optimum enzyme PH
- PH which enzymes work best at
- usually 7
Denatured enzyme
Enzyme which no longer works
Enzymes practical
- iodine drops in spotting tile
- syringe adds 1cm³ of amylase solution and 1cm³ of buffer solution with PH 5 to boiling tube
- place in boiling tube in 35° water bath for 5 minutes
- different syringe adds 5cm³ of starch solution to boiling tube
- mix boiling tube and start stop clock
- continuously sample how long it takes for amylase to break down starch
- use a pipette to drop fresh sample from boiling tube into iodine every 30 seconds until iodine is browny-orange
- repeat with different PHs
- calculate rate of reaction with 1000/time
What is amylase
A carbohydrase
What does amylase break down
Starch
What is starch broken down into
Maltose
Where is amylase made
- small intestine
- pancreas
- salivary glands
What does protease break down
Proteins
What is protein broken down into
Amino acids
Where is protease made
- small intestine
- pancreas
- stomach
What does lipase break down
Lipids
What are lipids broken down into
- 3 fatty acids
- 1 glycerol molecule