Other Analytical Methods Flashcards
(44 cards)
Excitation of electrons from lower to higher energy
Flame emission photometry
Determines the amt of light blocked
Turbidetry
Light source of of atomic absorption spectrophotometry
Hollow cathode lamp
Determines the amount of scattered light at various angles
Nephelometry
Unknown sample is made to react with a known solution in the presence of an indicator
Volumetric (titrimetric)
Measures light produced by atoms burned by a flame
Flame emission photometry
Standard pH
8.6
Chlorine test
Schales and schales method
Migration of charged particles in an electric field
Electrophoresis
Most common pH buffer
Pheno barbital/ veronal/barbital
Calcium test
Edta titration method
Used to scan and quantitate electrophoretic patterns
Densitometry
PH of tris boric edta
8.7
Stain for proteins and enZyme
Amino black, ponceau s
Ph of veronal
8.6
Stains for fat and lipoproteins
Oil red o / sudan black/ fat red 7B
Stain for csf proteins
Coomassie blue
Measures the absorbance of the molecules
Densitometry
Supporting media for isoelectric focusing
Agarose gel
Polyacrylamide gel
Cellulose acetate
Separates molecules through a pH gradient
Isoelectric focusing
2 forms of chromatography
Planar
Column
2 types of column chromatography
Gas
Liquid chromatography
Separation based on the sign and ionic charge density
Ion exchange chromatography
Chromatography for volatile substances
Gas chromatography