Outcomes 1 and 2

Question 1

Technique where vein is punctured by needle to withdraw blood or inject material

Answer 1

Venipuncture

Question 2

Process of entering the vein in order to remove blood

Answer 2

Phlebotomy

Question 3

Clotted specimens =

Answer 3

Serum

Question 4

Serum will not contain ____________, since it has been converted to _________ and is part of the clot.

Answer 4

Fibrinogen / Fibrin

Question 5

In order to form a clot, the following blood constituents are required:

Answer 5

Platelets, calcium, blood clotting factors, fibrinogen (a protein)

Question 6

Collection tube colours that yield serum:

Answer 6

Red, Gold, (SST), Tiger-topped, Orange (RST)

Question 7

Un-centrifuged, anticoagulated sample may also be known as:

Answer 7

Whole blood

Question 8

A centrifuged, anti-coagulated sample will yield:

Answer 8

Plasma

Question 9

Describe the 3 layers of a centrifuged, anticoagulated sample.

Answer 9

Supernatant is plasma, middle layer is buffy coat (white blood cells and platelets), precipitate is red blood cells

Question 10

Plasma contains _________ since the anticoagulant prevents it from converting to ______.

Answer 10

Fibrinogen / Fibrin

Question 11

Collection tube colours that yield plasma:

Answer 11

Lavender (CBC), Light Blue (Coag), Light and Dark Green (Chemistry), Grey

Question 12

Anticoagulant will remove either the ______ or the _______ from the plasma.

Answer 12

Calcium, thrombin

Question 13

Plasma or serum appears to be slightly pink to dark red. Red cells have burst. Most specimens are now unsuitable.

Answer 13

Hemolyzed specimen

Question 14

Plasma or serum appears milky or cloudy. Caused by increase in lipid in specimen, and avoided by collecting “fasting” specimens.

Answer 14

Lipemia

Question 15

Serum or plasma is golden or deep yellow colour, due to increase of bilirubin.

Answer 15

Icteric

Question 16

Gauge number possible range

Answer 16

20 - 25 gauge

Question 17

Most common gauge sizes for adult venipunctures

Answer 17

21 gauge (green) and 22 gauge (black)

Question 18

SESD

Answer 18

Safety Engineered Sharp Devices

Question 19

Solid colour Hemoguard lids indicate:

Answer 19

Fully evacuated tube (full draw)

Question 20

Translucent colour Hemoguard lids indicate:

Answer 20

Partially evacuated tube (low draw)

Question 21

Evacuated tubes volume ranges from:

Answer 21

1.2mL and 10mL

Question 22

Well mixed, anticoagulated (EDTA), not centrifuged

Answer 22

Whole blood

Question 23

anticoagulated and centrifuged to obtain the supernatant, which is

Answer 23

Plasma

Question 24

Specimens allowed to clot, and centrifuged to separate the clot (cellular content) from the supernatant, which is:

Answer 24

Serum

Question 25

Light blue tube additive

Answer 25

Na Citrate

Question 26

Na Citrate tube colour

Answer 26

Light Blue

Question 27

Light Green (PST) tube additive

Answer 27

Lithium heparin

Question 28

Lithium heparin tube colour

Answer 28

light green (PST)

Question 29

Lithium heparin tube colour

Answer 29

dark green

Question 30

dark green tube additive

Answer 30

lithium heparin

Question 31

sodium heparin tube colour

Answer 31

dark green

Question 32

dark green tube additive

Answer 32

sodium heparin

Question 33

Lavender tube additve

Answer 33

EDTA (Ethylene Diamine Tetra Acetic acid)

Question 34

EDTA tube colour

Answer 34

lavender

Question 35

grey tube additive

Answer 35

Na fluoride with K oxolate

Question 36

Na fluoride with K oxalate tube colour

Answer 36

Grey

Question 37

Plain red tube

Answer 37

Usually no additive, sometimes clot activator, serum

Question 38

usually no additive, serum

Answer 38

plain red tube

Question 39

SST

Answer 39

Serum Separator Tube

Question 40

Gold / SST / Red-Grey "tiger top"

Answer 40

barrier gel, serum

Question 41

regular barrier gel

Answer 41

gold, sst, tiger top tube

Question 42

orange/RST (Rapid separator tube)

Answer 42

gel barrier and thrombin to accelerate clotting, serum

Question 43

barrier gel and clot accelerator

Answer 43

orange / rst

Question 44

whole blood or blood culture recommended inversions

Answer 44

8

Question 45

citrated plasms (Light blue - Na citrate) recommended inversions

Answer 45

3-4

Question 46

Serum in red tube recommended inversions

Answer 46

0 for glass, 0-5 for plastic

Question 47

serum in gold (sst) tube recommended in versions

Answer 47

5

Question 48

heparinized plasma in green heparin tube recommended inversions

Answer 48

8

Question 49

whole blood in lavender (EDTA) tube recommended inversions

Answer 49

8

Question 50

oxolate/fluoride plasma in grey tubes recommended inversions

Answer 50

8

Question 51

Order of Draw

Answer 51

1. Blood cultures 2. Light blue (Na citrate) 3a. Serum in red b. Serum in Tiger top sst c. serum in gold sst 4a. Heparin plasma in light green b. Heparin plasms in dark green (lithium then sodium) 5. Lavender EDTA 6. Grey

Question 52

most desirable vein for venipuncture

Answer 52

median cephalic vein

Question 53

second most desirable vein for venipuncture

Answer 53

median cubital vein

Question 54

third most desirable vein to use for venipuncture

Answer 54

median vein

Question 55

not as desirable veins to use for venipuncture

Answer 55

basilic vein (close to nerve and artery) and cephalic vain (small and likes to roll)