Oxidative phosphorylation

Question 1

rotenone amytal inhibit what complex

Answer 1

complex I

Question 2

malonate inhibits

Answer 2

complex II

Question 3

antimycin A inhibits

Answer 3

complex III

Question 4

cyanide, azide and CO inhibi

Answer 4

complex IV

Question 5

oligomycin inhibits

Answer 5

complex V

Question 6

DNP, FCCp target

Answer 6

electrochemical gradient, uncouplers

Question 7

how to determine P/O

Answer 7

determine amoutn of O2 consumed after the addition of a known amt of ATP

Question 8

respiratory control

Answer 8

the dependence of repiration rate on the availability of ADP, the ATP synthase substrate

Question 9

RCR

Answer 9

ratio of slopes after and before ADP addition

Question 10

what is P/O a measure of?

Answer 10

mitochondrial efficiency

Question 11

respirasome

Answer 11

respiratory supercomplexes

Question 12

what influences the stability of respirasomes

Answer 12

mito specific lipid cardiolipin

Question 13

How does ATP synthases affect cristae morphology

Answer 13

ATP synthases assemble as dimers and greater, affecting the bend and curve of cristae and giving it its characteristic shape

Question 14

which subunit carries out Q cycle

Answer 14

complex III

Question 15

which subunit converts succinate to fumarate

Answer 15

complex II

Question 16

which subunit helps generate proton motive force through reduction of NADH

Answer 16

complex I

Question 17

which complex uses proton motive force power to convert ADP to ATP

Answer 17

complex V

Question 18

major type of pore in mito outer membrane

Answer 18

VDAC, aka porin. Permeable to most anionic metabolites

Question 19

Alias for complex I

Answer 19

NADH-Q oxidoreductase

Question 20

Alias for complex II

Answer 20

Succinate-Q reductase

Question 21

Alias for complex III

Answer 21

Q-cytochrome c oxidoreductase

Question 22

Alias for complex IV

Answer 22

cytochrome c oxidase

Question 23

Alias for complex V

Answer 23

ATP synthase

Question 24

which subunits generate electron transfer potential

Answer 24

I and II

Question 25

which subunites generate electrochemical potential

Answer 25

I, III, IV

Question 26

which sununits generate phosphoryl transfer potential

Answer 26

V

Question 27

two mobile e- carriers

Answer 27

Q and cytochrome c

Question 28

Complex I main action

Answer 28

Translocation of 3 protons using energy released by redox

Question 29

Complex II main action

Answer 29

Succinate to fumarate reduction coupled to reduction of FAD

Question 30

Complex III main action

Answer 30

(2) e- carrier to 2 (1) e- carriers with Q cycle, results in 2 protons pumped out and cyt c loaded with 2e- to complex IV

Question 31

Complex IV main action

Answer 31

Reduction of oxygen, pumps out 2H+

Question 32

Complex V main action

Answer 32

ATP synthase