page 2

Question 1

MEANS OF PERFORMING VENIPUNCTURE

Answer 1

● Syringe method
● Buttery infusion method
● Vacutainer/ETS method

Question 2

EQUIPMENTS OR MATERIALS NEEDED

In general:

Answer 2

Cotton/Gauze Pad,
Tourniquet,
70% ethyl
alcohol,
needle disposal container

Question 3

EQUIPMENTS OR MATERIALS NEEDED

For syringe method:

Answer 3

Syringe,
needles,
transfer device,
tubes

Question 4

EQUIPMENTS OR MATERIALS NEEDED

For evacuated method:

Answer 4

Tube holder,
needles,
evacuated tubes

Question 5

EQUIPMENTS OR MATERIALS NEEDED

For butterfly system:

Answer 5

winged infusion set

Question 6

Portable transillumination devices

Answer 6

Vein locating Device

Question 7

Inspecting the organ by passing light
through its walls

Answer 7

Transillumination

Question 8

how VEIN LOCATING DEVICE work

Answer 8

: Hemoglobin in the blood within the veins
absorbs the light, causing the vein to stand out as dark
lines

Question 9

Constrict the flow of blood and makes the vein more
prominent

Answer 9

TOURNIQUET

Question 10

Flat trip, non-latex made material

Answer 10

TOURNIQUET

Question 11

Buttery or Syringe collection

Answer 11

23 gauge

Question 12

● Single sample needle
● Sterile and disposable
● Larger the gauge number = smaller needle bore and
length
● 20-21 g are commonly used
● Color coding for needles indicates the gauge

Answer 12

NEEDLES/HYPODERMIC

Question 13

Can be reused
● 3-4 inches above puncture site (7.5-10cm)
● Not longer than one minute
● Blood pressure cuff (sphygmomanometer) for the obese,
pediatric, geriatric

Answer 13

tourniquet

Question 14

● Ivs or Blood Donation

Answer 14

16 and 18 gauge

Question 15

● Syringe or Evacuated system
collection

Answer 15

20, 21 or 22 gauge

Question 16

Intramuscular injections

Answer 16

25g

Question 17

PPD skin test

Answer 17

27g

Question 18

yellow

Answer 18

20

Question 19

green

Answer 19

21

Question 20

black

Answer 20

22

Question 21

blue

Answer 21

23

Question 22

orange

Answer 22

25

Question 23

brown

Answer 23

26

Question 24

Needle length:

Answer 24

○1 or 1.5 inch
○½ to ¾ inches - buttery needle

Question 25

Safety cap “Fishingout“ Syringe barrel that slides over the needle Device that slides over the needle after use

Answer 25

Syringe method

Question 26

Fragile, thin, or “rolly” veins that tend to ___

Answer 26

collapse

Question 26

● Glass or Plastic ● “ Breathing the syringe” ● “ The larger the syringe, the greater the amount of vacuum obtained.”

Answer 26

SYRINGE

Question 27

● 21g or 23 g ● ½ to ¾ inches ● Safety devices are sleeves that slides over the needle ● 5-12 inches rubber tubing

Answer 27

BUTTERFLY METHOD/SYSTEM

Question 27

Used for infants and children and patients with fragile veins (cancer patients) ● Could be connected to syringe or evacuated tubes

Answer 27

WINGED INFUSION SETS

Question 28

must be vertical when tubes are being filled in order to prevent blood in the tube from touching the needle in the ____

Answer 28

TRANSFER DEVICE

Question 29

Short needle with a thin tube with attached plastic wings

Answer 29

WINGED INFUSION SETS

Question 29

___ inches rubber tubing

Answer 29

● 5-12 inches

Question 29

● Multisample Needle/ Double-pointed needle ● Tube holder/needle adapter/ plastic holder ● Evacuated tubes

Answer 29

EVACUATED TUBE SYSTEM

Question 30

● Improper storage ● Opening the tube ● Dropping the tube ● Needle bevel partially out of skin

Answer 30

CAUSES OF PREMATURE LOSS OF VACUUM

Question 30

MULTI-SAMPLE NEEDLE SET/ DOUBLE POINTED NEEDLE

Answer 30

● Both ends are sharp ● The tube end has a rubber cover to prevent leakage between tubes ● 20,21,22 ( 21 and 22) ● Length: 1 to 1.5 inches Bevel (facing upward)

Question 30

● Help place and remove tubes ● Has an indentation about ¼ inch ● Grasp the same way of holding the barrel of syringe

Answer 30

EVACUATED TUBE SYSTEM

Question 31

● Sodalime, Borosilicate glass or plastic ● Tubes are indicated by color of rubber stopper (color-coded) ● With different additives or anticoagulant present ● Has vacuum inside ● Has expiration date ● Sterile

Answer 31

EVACUATED COLLECTION TUBES

Question 32

EVACUATED COLLECTION TUBES diameters

Answer 32

10,13,16 mm

Question 33

Pulling air from the tube ● Draw exact volume of blood indicated ● Loss of vacuum: fail to properly ll with blood

Answer 33

VACUUM

Question 34

TRUE OR FALSE Tubes do not fill blood all the way to the stopper

Answer 34

true

Question 34

● Preserve a specific blood constituent ● Used to improve sample quality or accelerate sample processing ● Aid in the separation of serum from cell

Answer 34

TUBE ADDITIVES

Question 35

Chelates or precipitates Calcium ● Inhibits thrombin activity

Answer 35

EDTA

Question 35

Mix or Number of Inversion Citrate

Answer 35

3-4 x

Question 36

● Interrupt the process of coagulation ● EDTA, Citrate, Heparin, Oxalates

Answer 36

ANTI COAGULANTS

Question 36

Most common anticoagulants used

Answer 36

●Ethylenediaminetetraacetic acid (EDTA) ● Citrate ● Heparin ● Oxalate

Question 37

● Prevents Glycolysis ● Sodium Fluoride

Answer 37

ANTI GLYCOLYTIC

Question 38

Mix or Number of Inversion EDTA and Heparin-

Answer 38

8x

Question 38

Anticoagulants with special use

Answer 38

Acid citrate dextrose- DNA testing, HLA typing, transplant compatibility ● Citrate phosphate dextrose ● Sodium polyanethol sulfonate (SPS)

Question 39

Combines calcium in a non-ionized form

Answer 39

citrate

Question 40

● Anti-coagulant ● Anti-complementary ● Anti-phagocytic ● Neutralizes aminoglycoside (antibiotic)

Answer 40

sodium polyanethol sulfonate

Question 41

inversions of sodium polyanethol sulfonate

Answer 41

8x

Question 42

Forming weakly dissociated calcium components ○Inhibits the glycolytic enzyme enolase

Answer 42

flouride

Question 43

● Combine with Calcium to form an insoluble salt.

Answer 43

oxalates

Question 44

● Acts as antithrombin and anti thromboplastin

Answer 44

heparin