paper and thin-layer chromatography

Question 1

Stationary phase: Plate (paper or tlc) in PAPER CHROMATOGRAPHY

Answer 1

Water (adhering on the paper)
**liquid-polar

Question 2

Stationary phase: Plate (paper or tlc) in THIN-LAYER CHROMATOGRAPHY

Answer 2

Silica (solid - polar)

Question 3

Mobile Phase (Solvent System) in PAPER CHROMATOGRAPHY

Answer 3

ethanol: ammonium hydroxide
** liquid - polar

Question 4

Mobile Phase (Solvent System) in THIN-LAYER CHROMATOGRAPHY

Answer 4

75% pet.ether: 25% acetone
** nonpolar

Question 5

Means of visualization in PAPER CHROMATOGRAPHY

Answer 5

spray w/ Ninhydrin

Question 6

Means of visualization in THIN-LAYER CHROMATOGRAPHY

Answer 6

UV Light

Question 7

What type of chromatography is a paper chromatography

Answer 7

Ascending

Question 8

What type of chromatography is a THIN-LAYER CHROMATOGRAPHY

Answer 8

Ascending

Question 9

Compound separated in PAPER CHROMATOGRAPHY

Answer 9

Amino Acids

Question 10

Compound separated in THIN-LAYER CHROMATOGRAPHY

Answer 10

Analgesics

Question 11

Advantages in PAPER CHROMATOGRAPHY

Answer 11

-Cheap
-best for polar compounds

Question 12

Advantages in THIN-LAYER CHROMATOGRAPHY

Answer 12

-Faster
-Develop more compact spots

Question 13

what will happen if the applied spots are too large

Answer 13

the developed spots will overlap and also results to tailing

Question 13

The portion of the paper which we happen to touch also develop violet spots. This means that sweat contains ________

Answer 13

amino acids

Question 13

what happens if the solvent level is above the applied spots

Answer 13

the spotted compounds will dissolve in the solvent system

Question 14

what will happen if the solvent is allowed to overrun the paper or TLC plate

Answer 14

we cannot compute the Rf value

Question 15

In TLC, the solvent system, the mobile phase is _______. ________, the stationary phase is polar

Answer 15

nonpolar

Question 16

The greater the Rf, _________

Answer 16

the more nonpolar the analgesic

Question 17

Formula for the Retention factor (Rf)

Answer 17

Rf = distance travelled by substance/ distance travelled by solvent

Question 18

what compound will elute first on the plate

Answer 18

compount with HIGHEST Rf value

Question 19

The Higher Rf, the ________ the compound, thus ________

Answer 19

less polar
basic

Question 20

relationship between Rf values and Polarity

Answer 20

inversely proportional

Question 21

why is it necessary to maintain a saturated vapor atmosphere in the developing chamber?

Answer 21

to stop the solvent from evaporating as it rises uo the paper. too much solvent evaporated from the plate and components will not move as far

Question 22

what can be done to improve the results in the experiment (paper and thin layer chromatography)

Answer 22

proper location and exact estimation of middle point must be done

Question 23

the physical separation of the components of a mixture on the basis of their differing affinities for a stationary phase vs. a mobile phase.

Answer 23

Chromatography

Question 24

In ________ chromatography, the mixture is spotted onto a sheet of paper

Answer 24

Paper

Question 25

paper chromatography works because of

Answer 25

the capillary action of water/solvent in the paper

Question 26

this allows us to separate compounds due to the attraction, or affinity, of the compounds for tge mobile phase vs the stationary phase

Answer 26

Capillary action

Question 27

the relative distance of travel of the substance to that of the solvent

Answer 27

Retention factor (Rf)

Question 28

It forms colored compounds with amino acids

Answer 28

Ninhydrin

Question 29

why does changing the solvent system would result toba different Rf value

Answer 29

altering the solvent system can change the relative positions of the separated compounds, resulting in different Rf values.

Question 30

the technique of separation and identification of chemical substance by a MOVING SOLVENT on a thin layer of suitable adsorbent with a binder serving as inert support for the layer

Answer 30

Thin-layer chromatography